Gphn (NM_172952) Mouse Untagged Clone

CAT#: MC221466

Gphn (untagged) - Mouse gephyrin (Gphn), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gphn
• Synonyms: 5730552E08Rik; AI662856; BC027112; C230040D23; geph; GPH; GPHRYN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221466 representing NM_172952
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCGAGGGAATGATCCTCACCAACCACGACCATCAAATCCGTGTCGGAGTCCTCACAGTGAGTG
  ATAGCTGCTTCAGGAATCTTGCAGAAGACCGCAGTGGGATAAATCTAAAAGATCTTGTCCAAGATCCTTC
  TTTGTTGGGTGGGACTATATCGGCATACAAGATAGTACCAGATGAAATAGAAGAAATCAAGGAAACACTC
  ATAGACTGGTGTGATGAAAAGGAACTTAATTTAATATTAACAACTGGAGGAACGGGGTTTGCACCACGAG
  ATGTCACTCCAGAGGCCACAAAAGAAGTAATAGAGCGGGAAGCGCCAGGGATGGCCCTGGCAATGCTGAT
  GGGATCGCTCAATGTCACACCTCTGGGCATGCTCTCTAGACCAGTGTGTGGAATAAGGGGGAAAACTCTG
  ATAATTAACCTACCTGGTAGCAAGAAAGGATCTCAGGAATGCTTTCAGTTCATACTGCCAGCTCTACCTC
  ATGCCATTGACCTTTTACGTGATGCCATTGTAAAAGTAAAGGAGGTGCATGATGAACTTGAAGATTTACC
  TTCCCCACCACCTCCTCTCTCTCCACCTCCTACAACTAGCCCACATAAACAGACAGAAGACAAAGGAGTT
  CAGTGTGAAGAAGAGGAAGAAGAAAAGAAAGACAGTGGTGTAGCTTCAACAGAAGATAGTTCCTCATCAC
  ATATAACTGCAGCAGCTCTTGCTGCAAAGATTCCAGACTCCATCATTTCTCGTGGTGTTCAGGTGCTCCC
  ACGAGACACAGCCTCCCTTAGCACTACTCCTTCAGAATCGCCCCGTGCTCAGGCTACATCTCGCCTCTCT
  ACAGCTTCTTGTCCAACACCAAAACAAATTAGACGGCCGGATGAAAGCAAAGGAGTTGCTAGTAGAGTTG
  GATCCCTCAAAGCTCGGCTTCCGTCGTGCTCATCTACCTATAGTGTATCTGAGGTCCAGTCCAGGTGCAG
  CAGCAAGGAGAACATTCTAAGAGCCAGTCACAGTGCTGTAGATATCACCAAGGTGGCTAGAAGACATCGC
  ATGTCTCCTTTTCCCCTGACGTCTATGGACAAAGCCTTCATTACAGTCCTGGAGATGACTCCGGTGCTTG
  GTACAGAAATCATCAATTACCGAGATGGAATGGGGCGAGTCCTTGCTCAAGATGTATATGCAAAAGACAA
  CCTACCCCCATTCCCTGCATCAGTAAAAGATGGCTATGCTGTTCGAGCTGCTGATGGTCCAGGGGATCGT
  TTCATCATTGGGGAATCCCAAGCTGGTGAGCAGCCAACTCAGACAGTAATGCCAGGACAAGTGATGCGGG
  TTACAACAGGTGCTCCAATCCCCTGCGGTGCTGATGCAGTAGTGCAAGTTGAAGATACTGAACTCATCAG
  GGAATCAGATGATGGTACTGAAGAACTTGAAGTACGAATTCTGGTGCAAGCTCGGCCAGGCCAAGATATC
  CGACCCATCGGCCATGACATTAAGAGAGGGGAGTGTGTTTTGGCCAAAGGAACCCATATGGGCCCTTCAG
  AGATTGGTCTCCTTGCAACTGTGGGTGTCACAGAAGTGGAAGTTAATAAGTTTCCAGTGGTTGCCGTTAT
  GTCAACAGGGAATGAGCTGCTAAATCCTGAAGATGATCTCTTACCAGGAAAGATTCGGGACAGCAATCGA
  TCAACACTTCTAGCAACAATTCAGGAACATGGTTACCCAACAATCAACCTGGGAATTGTAGGAGACAACC
  CAGATGACTTACTCAATGCCTTGAATGAGGGTATCAGCCGTGCTGATGTCATCATCACGTCAGGAGGTGT
  GTCCATGGGGGAAAAGGACTATCTCAAGCAGGTGCTGGACATTGATCTTCATGCTCAGATCCATTTTGGC
  AGAGTTTTTATGAAGCCAGGCTTGCCAACAACATTTGCAACTTTGGATATTGATGGTGTAAGAAAAATTA
  TTTTTGCACTACCAGGGAATCCTGTATCAGCTGTGGTCACCTGCAACCTCTTTGTTGTACCTGCACTGAG
  AAAGATGCAGGGTATCTTGGATCCTCGGCCAACCATCATCAAAGCCAGGTTATCGTGTGATGTAAAACTG
  GACCCTCGCCCAGAATACCACCGGTGTATACTGACTTGGCATCACCAAGAACCACTGCCCTGGGCCCAGA
  GTACAGGTAATCAGATGAGCAGCCGTCTGATGAGCATGCGCAGTGCCAATGGATTATTGATGCTACCTCC
  AAAGACAGAGCAGTACGTGGAACTTCACAAAGGCGAGGTGGTAGATGTCATGGTCATTGGACGGCTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172952
• Insert Size: 2310 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172952.3, NP_766540.2
• RefSeq Size: 3307 bp
• RefSeq ORF: 2310 bp
• Locus ID: 268566
• UniProt ID: Q8BUV3
• Cytogenetics: 12 C3
• Gene Summary: Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules. Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks one coding exon and contains two additional coding exons compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.