Igsf1 (NM_183335) Mouse Untagged Clone

CAT#: MC221456

Igsf1 (untagged) - Mouse immunoglobulin superfamily, member 1 (Igsf1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Igsf1
• Synonyms: 5330413N23; 5530402E03; AI747649; InhBP/p120; mKIAA0364
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221456 representing NM_183335
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCTTCGGACCTTCACTCTTTTGCTCCTTTGCATTTGGCTCAATCCGGGTATGACTTCACTGGCAG
  TGGAGTCTCAACCAGAGCTATGGATAGAGTCCAACTACCCCCAGGCCCCTTGGGAGAACATCACACTCTG
  GTGCAAAAGCCCCTCTCGGGTATCCAGCAAGTTCCTGCTTCTGAAGGATAACTCACAGATGACCTGGATT
  CGTCCTCCTTACAAGACATTCCAAGTTTCATTCTTCATAGGTGCCCTTACTGAGTCCAATACAGGTCTTT
  ATCGATGCTGCTACTGGAAGGAGAAAGGCTGGTCAAAACCCAGTAAAATTCTAGAGCTAGAAGCACCAGG
  CCAACTCCCTAAACCCATCTTCTGGATCCAGGCAGAGACCCCCCCTCTTCCTGGATGCAATGTTAACATC
  TTCTGCCATGGATGGCTCCAGGATTTGGTATTTATGCTGTTTAAAGAGGGATACACAGAGCCCGTAGATT
  ACCAAGTCCCAACTGGGACAATGGCCATCTTCTCCATTGATAACTTGGCACCCGAGAATGAAGGGGTTTA
  CATCTGCCGCACTCATATCCAGATGCTCCCCACTCTGTGGTCAGAGCCCAGCAATCCCCTGAAGTTGGTG
  GTAGCAGGTCTCTACCCCAAACCAACTCTGACAGCCCATCCTGGGCCCATCCTGGCACCCGGAGAAAGCC
  TGAGTCTCAGGTGTCAAGGGCCAATATATGGAATGACGTTTGCTTTAATGAGGTTGGAAGACTTGAAGAA
  ATCATTTTACCACAAGAAGCCAATAAAAAATGAGGCATATTTCTACTTCCAGGATCTGAAGATACAGGAT
  ACAGGGCACTACCTCTGTTTTTACTATGATGGGTCATACAGGGGCTCTCTGCTTAGTGATATCCTGAAAA
  TCTGGGTGACTGACACTTTCCCTAAGACCTGGCTACTTGTTCAGCCCAGTCCTGTGATTCAAATGGGTCA
  GAACGTGAGCCTGAGGTGTGGAGGACTGATGGATGGAGTGGGTCTTGCACTCTACAAGAAGGGAGAAGAA
  AAGCCCCTTCAGTTTCTGGATGCCTCCAGCAACACTGGCAACAATTCATTCTTCCTAAAGAATGTGACCT
  ACAGGGATGCTGGCATCTATAGCTGTCACTATTATCTTACTTGGAAGACATCCATTAAGATGGCAACATA
  CAACACTGTGGAGCTGATGGTTGTAGCTTGGCCCAGTTCCGTGTTCAAAGTAGGAAAGACCATCACCCTT
  CAGTGCCGAGTGTCTCATCCAGTGCTTGAATTTTCTTTGGAATGGGAAGAAAGAACAACATTCCAAAAAT
  TCTCAGTAGATGGAGACTTCCTCATCACTGACATTGAAGGGCAAGGCACAGGAACCTACAGTTGCAGCTA
  TCGTATTGAGGCACACCCTAACACCTGGTCACATCGCAGTAAGCCTCTGAAGTTGGTGGGACCAGCAGGC
  TTCCTCACCTGGAATTCCATTCTGAATGAAGCTGTCAGGGTGTCCTTAACCATGCAGCTTGCTTCCTTGC
  TCTTGCTAGTGGTATGGATCCGGTGGAAGTGTCGGAGACTGAGGCTCAGAGAAGCCTGGTTGTTGGGAAC
  AGCTCAAGGGGTCGCCATGCTCTTCATACTCATGGCCCTTCTTTGCTGTGGACTGTGCAATGGGGCATTG
  ACAGAGGAGATTGAAATAGTCATGCCAACCCCTAAGCCTGAACTGTGGGCAGAGACGAACTTCCCTCTGG
  CCCCGTGGAAGAACTTAACCCTCTGGTGCAGAAGCCCATCTGGCTCAACTAAGGAGTTTGTGTTACTGAA
  GGACGGGACCGGGTGGATTGCAACCCGCCCGGCCTCAGAGCAGGTCCGGGCTGCCTTCCCTCTTGGCGCC
  CTGACCCACAGCCACACCGGGAGTTACCATTGCCATTCATGGGAGGAGATGGCTGTGTCGGAGCCTAGTG
  AAGCGCTTGAACTAGTGGGGACAGACATCCTTCCCAAACCTGTCATTTCGGCTTCCCTTCCAATCCGGGG
  CCAGGAACTGCAGATCCGCTGTAAAGGATGGCTGGAAGGTTTGGGGTTTGCTCTGTATAAGAAGGGAGAG
  CAGGAACCTGTCCAGCAACTTGGTGCTGTTGGGAGAGAAGCCTTCTTTACAATTCAAAGAATGGAGGATA
  AAGATGAGGGCAATTACAGCTGCCGCACACACACTGAAATGCAGCCCTTCAAGTGGTCTGAGCCCAGTGA
  GCCCCTGGAGCTTGTCATAAAAGATGGGCGAACCAAGGCTCAGAACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_183335
• Insert Size: 2289 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183335.2, NP_899178.2
• RefSeq Size: 2513 bp
• RefSeq ORF: 2289 bp
• Locus ID: 209268
• UniProt ID: Q7TQA1
• Cytogenetics: X A5
• Gene Summary: Seems to be a coreceptor in inhibin signaling, but seems not to be a high-affinity inhibin receptor. Antagonizes activin A signaling in the presence or absence of inhibin B. Necessary to mediate a specific antagonistic effect of inhibin B on activin-stimulated transcription (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks most of the 5' UTR and uses a different segment for its 3' coding region and 3' UTR, compared to variant 1. The resulting protein (isoform 3) has a shorter and distinct C-terminus when it is compared to isoform long.