Top1 (NM_009408) Mouse Untagged Clone

CAT#: MC221438

Top1 (untagged) - Mouse topoisomerase (DNA) I (Top1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Top1
• Synonyms: AI467334; D130064I21Rik; Top-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221438 representing NM_009408
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGGGACCATCTCCACAACGATTCCCAGATCGAAGCGGATTTCCGATTGAATGATTCTCACAAAC
  ACAAAGATAAACACAAAGATCGAGAACACCGGCATAAGGAGCACAAGAAGGATAAGGATAAGGACCGGGA
  AAAGTCTAAGCATAGCAACAGTGAACATAAAGATTCTGAAAAGAAACACAAAGAGAAAGAGAAAACCAAA
  CACAAAGATGGCAGCTCAGAAAAACATAAAGACAAACATAAAGACAGAGACAAGGAAAGACGAAAGGAGG
  AAAAGATTAGAGCTGCTGGGGATGCAAAAATAAAGAAGGAAAAGGAAAATGGCTTCTCTAGTCCGCCACG
  AATTAAAGATGAACCTGAGGATGATGGCTATTTTGCTCCTCCAAAAGAGGATATTAAGCCATTGAAGAGG
  CTTCGAGATGAAGATGATGCTGATTATAAACCTAAGAAAATTAAGACAGAAGATATCAAGAAGGAGAAGA
  AAAGAAAATCAGAAGAGGAAGAGGATGGTAAACTTAAAAAGCCCAAGAATAAAGATAAAGATAAAAAAGT
  TGCTGAGCCAGATAATAAGAAAAAGAAGCCGAAAAAGGAAGAGGAACAGAAGTGGAAATGGTGGGAAGAA
  GAACGTTATCCAGAAGGCATCAAATGGAAATTCCTAGAGCATAAAGGGCCTGTCTTCGCTCCACCATATG
  AGCCTCTGCCAGAGAGTGTCAAGTTTTACTATGATGGTAAAGTTATGAAGCTGAGTCCTAAAGCAGAAGA
  AGTAGCTACATTCTTTGCAAAAATGCTTGACCACGAATATACTACTAAGGAAATATTCAGGAAAAATTTC
  TTTAAAGATTGGAGAAAGGAAATGACTAATGACGAAAAAAATACGATTACCAACCTAAGCAAATGCGACT
  TTACACAGATGAGCCAGTATTTCAAAGCGCAGTCAGAAGCTCGGAAACAGATGAGCAAGGAAGAAAAATT
  GAAAATCAAAGAAGAAAATGAAAAGTTATTGAAAGAATACGGCTTTTGTGTAATGGATAATCATAGAGAG
  CGGATTGCCAACTTCAAGATAGAGCCTCCGGGGCTTTTCCGAGGCCGAGGGAACCACCCCAAGATGGGTA
  TGCTGAAGAGAAGGATCATGCCTGAGGACATCATCATCAACTGTAGCAAAGACGCAAAGGTTCCTTCTCC
  CCCTCCTGGACATAAATGGAAGGAAGTCCGACATGATAACAAGGTTACTTGGCTGGTCTCCTGGACAGAG
  AATATCCAAGGTTCTATCAAATATATCATGCTGAATCCCAGTTCACGAATCAAGGGTGAGAAAGACTGGC
  AGAAGTATGAGACTGCGCGGCGTCTGAAGAAGTGTGTGGACAAGATCCGAAATCAGTATCGGGAAGACTG
  GAAGTCCAAAGAGATGAAAGTTCGTCAGAGAGCTGTAGCACTGTACTTCATTGATAAGCTTGCTCTGAGA
  GCAGGCAATGAGAAGGAAGAAGGAGAGACGGCAGACACTGTGGGCTGCTGTTCACTTAGAGTGGAACACA
  TCAATTTACACCCAGAATTGGATGGTCAGGAGTATGTGGTGGAATTTGACTTTCCTGGGAAGGACTCAAT
  CAGATACTATAACAAAGTCCCAGTTGAGAAACGAGTTTTTAAAAACTTACAACTGTTTATGGAGAACAAG
  CAGCCTGAGGACGATCTTTTTGATCGACTTAATACTGGTATTCTAAATAAACATCTTCAGGATCTCATGG
  AGGGCTTGACTGCCAAGGTGTTCCGTACCTACAATGCCTCCATCACACTACAGCAGCAGCTTAAAGAGCT
  CACAGCCCCTGATGAGAATGTACCAGCGAAGATTCTATCTTATAACCGTGCCAATCGAGCTGTTGCAATT
  CTTTGTAACCACCAGAGGGCGCCACCAAAGACCTTTGAGAAGTCAATGATGAACTTGCAGTCTAAGATTG
  ATGCCAAGAAAGATCAGTTAGCAGATGCTCGAAGGGACCTGAAAAGTGCTAAGGCTGATGCCAAAGTCAT
  GAAGGATGCAAAGACCAAGAAGGTAGTAGAGTCAAAAAAGAAGGCTGTACAGAGACTAGAAGAGCAGTTG
  ATGAAGCTGGAAGTTCAAGCCACAGATCGAGAGGAGAACAAACAAATTGCCTTGGGGACCTCCAAACTCA
  ATTATCTGGACCCTAGGATCACAGTGGCTTGGTGCAAAAAGTGGGGGGTCCCAATTGAGAAGATTTACAA
  CAAAACCCAGAGGGAGAAGTTTGCTTGGGCCATTGATATGACTGATGAGGACTACGAGTTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009408
• Insert Size: 2304 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009408.2, NP_033434.2
• RefSeq Size: 3859 bp
• RefSeq ORF: 2304 bp
• Locus ID: 21969
• UniProt ID: Q04750
• Cytogenetics: 2 80.96 cM
• Gene Summary: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.[UniProtKB/Swiss-Prot Function]