Gria2 (NM_001039195) Mouse Untagged Clone

CAT#: MC221425

Gria2 (untagged) - Mouse glutamate receptor, ionotropic, AMPA2 (alpha 2) (Gria2), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gria2
• Synonyms: Glu; GluA2; GluR; Glur-2; GluR-B; gluR-K2; Glur2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221425 representing NM_001039195
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAAAGATTATGCATATTTCTGTCCTCCTTTCTCCTGTTTTATGGGGACTGATTTTTGGTGTCTCTT
  CTAACAGCATACAGATAGGGGGGCTATTTCCAAGGGGCGCTGATCAAGAATACAGTGCATTTCGGGTAGG
  GATGGTTCAGTTTTCCACTTCGGAGTTCAGACTGACACCCCATATCGACAATTTGGAGGTAGCCAACAGT
  TTCGCAGTCACCAATGCTTTCTGCTCCCAGTTTTCAAGAGGCGTCTATGCGATTTTTGGGTTTTACGACA
  AGAAGTCTGTAAATACCATCACATCATTCTGTGGGACACTGCATGTATCCTTCATCACACCAAGCTTCCC
  AACAGATGGCACGCATCCATTTGTCATCCAGATGCGACCTGACCTCAAAGGAGCACTCCTTAGCTTGATT
  GAGTACTACCAATGGGATAAGTTCGCATACCTCTATGACAGTGACAGAGGCTTATCAACACTGCAAGCTG
  TGCTGGATTCTGCTGCGGAGAAGAAGTGGCAGGTGACTGCTATCAATGTGGGGAACATTAACAATGACAA
  GAAAGATGAGACCTACAGATCACTCTTTCAAGATCTGGAGTTAAAAAAAGAACGGCGTGTAATCCTTGAC
  TGCGAAAGGGATAAAGTCAATGACATTGTGGACCAGGTTATTACCATTGGAAAGCATGTTAAAGGGTACC
  ATTATATCATTGCAAATCTGGGATTTACTGATGGAGACCTGCTGAAAATTCAGTTTGGAGGAGCAAATGT
  CTCTGGATTTCAGATTGTAGACTACGACGACTCCCTGGTGTCTAAATTTATAGAAAGATGGTCAACACTC
  GAAGAGAAAGAATACCCTGGAGCACACACAGCGACAATTAAGTATACTTCGGCCCTGACTTATGATGCTG
  TCCAAGTGATGACTGAAGCATTCCGCAATCTTCGGAAGCAGAGGATTGAAATCTCCAGGAGAGGAAATGC
  AGGAGATTGTTTGGCCAACCCAGCTGTGCCTTGGGGACAAGGCGTGGAAATAGAAAGGGCCCTCAAGCAG
  GTTCAAGTTGAAGGTCTCTCTGGAAATATAAAATTTGACCAGAACGGAAAACGAATAAACTACACAATTA
  ACATCATGGAGCTCAAAACAAATGGACCCCGGAAGATTGGGTACTGGAGTGAAGTGGATAAAATGGTTGT
  CACCCTAACCGAGCTCCCCTCTGGAAATGACACATCTGGGCTTGAAAACAAAACTGTGGTTGTCACCACA
  ATATTGGAATCTCCATATGTTATGATGAAGAAAAATCATGAAATGCTTGAAGGGAATGAGCGTTATGAGG
  GCTACTGTGTTGACTTAGCTGCAGAAATTGCCAAACATTGTGGATTCAAGTACAAGCTGACTATTGTTGG
  GGATGGCAAGTATGGGGCCAGGGATGCAGACACCAAAATTTGGAATGGTATGGTTGGAGAACTTGTATAT
  GGGAAAGCTGATATTGCCATTGCTCCATTAACTATCACTCTCGTGAGAGAAGAGGTGATTGACTTCTCGA
  AGCCATTCATGAGCCTTGGAATCTCTATCATGATCAAGAAGCCTCAGAAGTCCAAACCAGGAGTGTTTTC
  CTTTCTTGATCCTTTAGCCTATGAGATCTGGATGTGCATTGTGTTTGCCTACATTGGGGTCAGTGTAGTT
  TTATTCCTGGTCAGCAGATTTAGCCCCTACGAGTGGCACACTGAGGAATTTGAAGATGGAAGAGAAACAC
  AAAGTAGTGAATCAACTAATGAATTTGGGATTTTTAATAGTCTCTGGTTTTCCTTGGGTGCCTTTATGCG
  GCAAGGATGCGATATTTCGCCAAGATCTCTCTCTGGGCGCATTGTTGGAGGTGTGTGGTGGTTCTTTACC
  CTCATCATCATCTCCTCCTACACGGCTAACTTAGCTGCCTTCCTGACTGTAGAGAGGATGGTGTCGCCCA
  TCGAAAGTGCTGAGGATCTGTCTAAGCAAACAGAAATTGCTTATGGAACATTAGACTCTGGCTCCACTAA
  AGAGTTTTTCAGGAGATCTAAAATTGCAGTGTTTGATAAAATGTGGACTTATATGAGGAGTGCAGAGCCC
  TCTGTGTTTGTGAGGACTACGGCAGAAGGAGTAGCCAGAGTCAGGAAATCCAAAGGGAAGTATGCCTACT
  TGCTGGAGTCCACAATGAATGAGTACATCGAGCAGAGGAAGCCTTGCGACACCATGAAAGTGGGCGGCAA
  CCTGGATTCCAAAGGCTACGGCATCGCCACACCTAAAGGATCCTCATTAAGGTGGGTGGAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001039195
• Insert Size: 2304 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039195.1, NP_001034284.1
• RefSeq Size: 3491 bp
• RefSeq ORF: 2304 bp
• Locus ID: 14800
• Cytogenetics: 3 35.5 cM
• Gene Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) differs at the 3' end compared to transcript variant 1, and encodes a shorter isoform (3) with a distinct C-terminus compared to isoform 1. RNA editing (CAG->CGG) changes Gln607Arg. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.