Bard1 (NM_007525) Mouse Untagged Clone

CAT#: MC221420

Bard1 (untagged) - Mouse BRCA1 associated RING domain 1 (Bard1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bard1
• Synonyms: ENSMUSG00000060893; ENSMUSG00000073653
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221420 representing NM_007525
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACGCCGGCCGCCGAGGGTCTGCTCTGGGAACCAGCCTGCTCCCGTGCCCGCCATGGAGCCGGCTA
  CCGACGGGCTTTGGGCCCACAGCCGCGCGGCGCTTGCCCGCCTGGAGAAGCTGCTGCGCTGCTCCCGCTG
  TGCTAATATTCTGAAGGAGCCCGTGTGCTTAGGAGGATGTGAGCACATCTTCTGTAGTGGTTGTATAAGT
  GACTGTGTTGGCTCAGGATGCCCAGTGTGTTACACCCCAGCCTGGATCCTAGACCTCAAGATAAACCGAC
  AATTGGACAGCATGATCCAGCTTTCTAGTAAGCTCCAAAATTTGCTCCATGACAATAAAGATTCAAAAGA
  CAACACATCTAGGGCAAGTTTATTTGGTGATGCAGAAAGGAAGAAGAATTCAATAAAAATGTGGTTTAGT
  CCTCGAAGTAAGAAGGTTAGATATGTTGTGACTAAAGTGTCAGTACAAACACAGCCTCAAAAGGCAAAGG
  ACGACAAAGCCCAGGAAGCCTCAATGTATGAATTTGTTTCCGCAACTCCCCCTGTAGCTGTTCCTAAGAG
  TGCTAAAACAGCTTCTAGAACATCTGCAAAAAAGCATCCAAAGAAATCTGTAGCTAAGATCAACAGGGAG
  GAGAATTTAAGGCCAGAAACAAAGGATAGTAGATTTGATTCCAAAGAGGAGCTGAAGGAAGAGAAGGTTG
  TCTCCTGTAGCCAAATACCAGTTATGGAGAGACCACGGGTAAATGGTGAAATAGACTTATTAGCAAGTGG
  TTCTGTTGTAGAACCTGAATGTTCTGGAAGCTTGACTGAAGTCTCTTTACCATTGGCTGAGCATATAGTG
  TCTCCAGACACTGTGAGCAAGAATGAAGAGACTCCGGAGAAGAAGGTCTGTGTAAAAGATCTTCGTTCAG
  GAGGGAGTAATGGAAATCGTAAAGGCTGCCACAGGCCTACCACTTCTACTTCTGACAGTTGTGGGAGCAA
  CATTCCGAGCACCAGCAGAGGCATCGGTGAGCCAGCATTGCTTGCAGAAAACGTAGTGTTGGTTGACTGC
  TCTTCACTGCCTTCAGGCCAGCTACAGGTTGATGTCACACTCAGGAGAAAGAGTAACGCATCAGATGACC
  CCCTTAGCCTTTCACCGGGCACACCCCCACCTCTGCTGAACAATTCAACTCACAGACAAATGATGTCAAG
  CCCCTCCACAGTGAAGCTGTCTTCTGGTATGCCAGCCAGGAAAAGAAATCACAGAGGAGAGACCTTACTT
  CATATTGCTTCTATTAAGGGTGATATACCTTCTGTTGAATACCTCTTGCAAAATGGAAACGACCCAAATG
  TTAAAGACCATGCTGGATGGACACCGTTGCATGAAGCCTGCAGTCATGGGCACCTGAAGGTAGTGGAGTT
  GCTGCTCCAGCATAATGCCCTGGTGAACACCCCTGGCTATCAGAATGACTCGCCACTCCACGATGCCGTC
  AAGAGTGGCCACATCGATATAGTCAAGGTGTTACTGTCCCACGGTGCTTCCAGGAATGCTGTTAACATAT
  TTGGTGTGCGGCCTGTGGACTATACAGACAATGAGAATATAAGATCATTATTGCTGCTGCCAGAGGAGAA
  TGAATCATTCTCAACTAGCCAGTGCTCTATCGTGAACACCGGGCAGCGAAAGAATGGGCCGCTGGTATTT
  ATAGGCAGTGGGCTTTCTTCACAGCAGCAGAAAATGCTCAGCAAACTTGAGACAGTTCTTAAGGCTAAAA
  AGTGTATGGAATTTGACAGTACAGTAACTCATGTCATTGTTCCTGATGAGGAAGCGCAGAGTACTCTGAA
  GTGTATGCTTGGGATTCTCAGTGGATGCTGGATCCTGAAGTTTGATTGGGTGAAAGCCTGTCTGGACAGC
  AAAGTACGTGAGCAGGAAGAAAAGTATGAAGTTCCTGGAGGTCCACAGAGGAGCAGGCTCAACAGAGAGC
  AGCTGTTGCCAAAGCTGTTTGATGGATGCTACTTCTTTTTGGGGGGAAACTTCAAGCATCATCCAAGGGA
  TGACCTCCTTAAGCTCATTGCTGCAGCAGGAGGCAAAGTCCTGAGTCGCAAGCCCAAGCCAGACAGTGAT
  GTGACTCAGACCATCAACACGGTTGCATACCATGCCAAGCCTGAGTCGGATCAGCGCTTCTGTACGCAGT
  ACATCGTCTACGAAGACCTGTTTAACTGTCACCCAGAGAGGGTTCGGCAGGGCAAAGTCTGGATGGCTCC
  GTCCACCTGGCTTATCAGCTGTATAATGGCCTTTGAATTGCTTCCTCTTGACAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007525
• Insert Size: 2298 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC128370, AAI28371
• RefSeq Size: 2386 bp
• RefSeq ORF: 2298 bp
• Locus ID: 12021
• UniProt ID: O70445
• Cytogenetics: 1 C3
• Gene Summary: E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.[UniProtKB/Swiss-Prot Function]