Tmc8 (NM_001195088) Mouse Untagged Clone

CAT#: MC221396

Tmc8 (untagged) - Mouse transmembrane channel-like gene family 8 (Tmc8), transcript variant A, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tmc8
• Synonyms: Ever2; EVIN2; mFLJ00400
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221396 representing NM_001195088
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAAAGATTGTGTTTTTCTAAAGCGTAAGGGCCAAGGTGGAGCTCCTGCGAGCAAGGATCTTGCCT
  GTCTGTCCCGCGCGCCCACTGGGATGTTTCGCCAGTGGTCGGTGCAGTCAGGACCAGCCCCACGAAGGCC
  TGAATCCCAGGCGGCCTCGGAGGAGCTGTGGGAGCAAGAAGTGGAGCGGCTATGCGCCTCCCGGACGCCT
  GTGCGGATGCTGCCTTACGCCATGGCAGACAAGCGCTTCATCCGGGAACTGCGGGAGCCCGAGGGAGTGA
  AGACCACCTTCTGGCAGCGGTGGCATCGCCCGAGGCGGGTGGCGCGACAACACCTGAGGGAGGCAGAGCA
  ACGGCTGGCCCGCGGCTTCGGTCTCTGGGAGGGAGCTCTCTACGAGATTGGGGGCCTCTTCGGAACCGGG
  ATCCAGTCCTACTTCACTTTCCTTCGCTTCCTGCTGCTGCTCAACCTGCTGACCATGCTGCTGACTGCCT
  GCTTTGTGCTGCTGCCCCTGGTCTGGCTCCGCCCCCCTGAGCTAGGCCCTGCCCTGAAACTGACAGGACT
  CCAGTGCTCCAGCAGCCCCCTGCCTCAGAGTGACATTCCAAGGTTTCACAACCCACTTTGGAATATTTTA
  ACGGGCAGAGCCTTCAACAACACCTATCTCTTCTACGGTGCCTACCGAGCGGGGCCAGAGAGCAGCTCTG
  AATACAGCATCCGCCTGGCCTACCTCCTTAGCCCGATGGTCTGCCTGCTCCTCTGTTTCTGTGGGATACT
  GCAGAGGATGGCAGAAGGCCTACCCCAGCAGACACTCTTGGGTCAGCGCTACAGGACACCCCTCAGCGCC
  AAAGTGTTCTCATCCTGGGACTTCTGCATCCGGGTGTGGGAAGCCGCCACCATCAAGAAGCACGAAATCA
  GCAATGAACTGAAGATGGAGCTGGAAGAGGGCCGCCGCGTGGAGCTGGCTCAACAACAGACCAGGGCCCA
  GAAGGCCTGCCGCCTGCTCACCTACCTTCGCACCAACATCCTCATTGTGCTCCTAGTGGTCGGAGCCATC
  AGCGCCATCTTCTGGGCCACCAAGTACTCACAGGACAACAAGGAGGAGTCCCTTTTTCTGGTGCTCCAGT
  ACTTGCCTCCTGGAGTCATCTCCCTGGTCAACTTCCTGGGCCCCCAATTGTTCACAGTCCTGATCCAGCT
  GGAGAACTACCCTCCCGGCACTGAAGTCAACCTCACCCTGATCTGGTGCGTGGTGCTGAAGCTGGCCAGC
  TTGGGCATGTTCTCCTTCTCCCTGGGTCAGACGGTGCTGTGCATAGGCAGAAACAAGACCAGCTGCGAGT
  CCTACGGCTACAACGCTTGCGACTACCAGTGCTGGGAGAACTCGGTGGGCGAAGAATTATATAAGTTAAT
  CATCTTCAACTTCCTGCTCACCGTGGCCTTCGCCTTCCTGGTCAGCCTGCCTCGAAGGCTGTTAGTGGAG
  CGCTTCTCGGGCTGGTTCTGGACTTGGCTAGACCGGGAGGAGTTCCTGGTGCCTAAGAATGTGCTGGACA
  TCGTGGCTGCACAGACTGTCACCTGGATGGGCCTCTTCTACTGCCCCCTGCTGCCACTACTCAACAGTGT
  CTTCCTCTTTCTCACCTTCTACATCAAGAAGTACACCCTCCTGAGGAACTCCAGGGCGTCCCCGCGGCGT
  TTCCGAGCCTCCAGCTCCACCTTCTTCTTCCACCTGGTGCTGCTCCTAGGCCTGCTTTTGGCTGCAGTAC
  CTCTGGCCTACGTGATCAGCAGCACCCACTCCTCCTGGGACTGTGGCCTCTTCACAAACTATTCGGCCCC
  CTGGCAGGTGGTACCCGAGCTGGTAGCCCTCCAGCTCCCACTTCCTAGCCAGCGTGCCCTCCGTTACCTC
  AGCTCCCACGCCTTCAGCTTCCCACTCCTCATTCTGCTCAGCATCGTCCTGACGGTTTGCATCTCCCAGT
  CCCGGGCGAATGCCAGAGCCATCCAGGGGCTGAGGAAGCAACTGGTGTGGCAGGTCCAGGAAAAGTGGCA
  CCTGGTGGACGACCTGTCGCGGCTGCTGCCAGAACTGAGCCCAGAGCCCGGGTCCCCTCACTCTCGAGCT
  TCGCGTCCACGATCCTTCTGTCCTGGATTTCCATGCCCAGGCTCGCCCGGTCCCAGGACACCCAGGCTGG
  CACCCTCCAACAGGCTGAGCTCCTCGAGCCTCGGAGCCCCCAGTGCCTCGGTTCCTGCCTCCAGATTTCA
  CTTCCCCAGCCGCACAGAGCTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001195088
• Insert Size: 2265 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195088.1, NP_001182017.1
• RefSeq Size: 2790 bp
• RefSeq ORF: 2265 bp
• Locus ID: 217356
• Cytogenetics: 11 E2
• Gene Summary: Probable ion channel.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (A) represents the longest transcript and encodes the longest isoform (A). Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.