Thap12 (NM_028410) Mouse Untagged Clone

CAT#: MC221387

Prkrir (untagged) - Mouse protein-kinase, interferon-inducible double stranded RNA dependent inhibitor, repressor of (P58 repressor) (Prkrir), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Thap12
• Synonyms: 2900052B10Rik; Dap4; Prkrir; Rpkrir
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221387 representing NM_028410
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAACTTCTGCGCTGCCCCCAACTGCACACGGAAGAGCACGCAGTCTGACCTGGCCTTCTTCAGGT
  TCCCGCGGGACCCGGCCAGATGCCAGAAGTGGGTTGAGAATTGTAGGAGAGCAGACTTAGAAGATAAAAC
  ACCAGATCAACTAAATAAACATTATCGACTTTGTGCCAAACACTTTGAGACCTCTATGATTTGTAGAACT
  AGTCCTTATCGGACAGTCCTTCGAGATAATGCAATACCAACAATATTTGATCTTACCAGTCATTTGAACA
  ACCCGCATAGCAGACACAGAAAACGAATAAAAGAATTGAGTGAAGATGAAATCAGGACTCTGAAACAGAA
  AAAAATTGAGGAGACTTCTGAACAAGAGCAGGAAACTAACACCAATGCTCAGAACCCCAGTGCAGAAGCG
  GTGAACCAGCAGGATGCAAATGTGCTGCCTTTAACCCTTGAAGAGAAGGAGAACAAAGAGTACCTCAAGT
  CTTTATTTGAGATTCTGGTTCTCATGGGAAAGCAGAACATCCCCTTGGATGGGCACGAGGCTGACGAGGT
  CCCCGAGGGCCTCTTTGCCCCAGACAACTTCCAGGCGCTCCTGGAGTGCCGCATCAACTCTGGCGAGGAG
  GTCCTAAGGAAGCGCTTTGAGGCCACAGCGGTCAACACATTATTCTGCTCTAAAACTCAGCAGAGGCACA
  TGCTGGAGATCTGTGAGAGCTGCATTCGGGAGGAGACTCTCAGGGAAGTGAGGGACTCACACTTCTTTTC
  CATTATCACAGACGATGTGGTGGACATAGCAGGAGAAGAGCACCTGCCCGTGCTGGTGAGGTTTGTCGAT
  GACGCCCATAACCTGAGAGAGGAGTTTGTGGGATTTCTGCCGTATGAAGCTGATGCGGAGATTTTGGCTG
  TGAAATTTCACACTACAATAACTGAGAAATGGGGATTGAATATGGAGTATTGTCGTGGTCAGGCTTACAT
  TGTGTCCAGTGGATTTTCTTCCAAAATGAAAGTTGTTGCTTCAAGACTTTTAGAAAAATATCCCCAGGCT
  GTCTATACCCTCTGTTCTTCCTGTGCCTTAAATGCATGGTTGGCAAAGTCTGTCCCTGTGATCGGGGTGT
  CCGTGGCTTTAGGAACCATTGAGGAGGTCTGTTCTTTTTTCCATCGATCACCACAGCTGCTTTTAGAACT
  TGACAGTGTAATTTCTGTTCTTTTTCAGAATAGTGAAGAACGGGCTAAAGAACTGAAGGAAATTTGTCAT
  TCCCAGTGGACAGGCAGGCATGATGCCTTTGAAATCTTAGTGGATCTCCTACAAGCACTTGTCTTATGTT
  TAGATGGTATAATAAATAGTGACACAAATGTTAGGTGGAATAACTATATAGCTGGGCGAGCATTTGTCCT
  CTGTAGTGCAGTGACAGACTTTGATTTCATTGTTACCATTGTTGTTCTTAAAAATGTGTTATCTTTTACA
  AGAGCCTTTGGGAAGAACCTCCAGGGACAGACCTCTGACGTCTTCTTTGCAGCCAGCAGCCTGACTGCAG
  TACTGCATTCACTTAATGAGGTCATGGAAAATATTGAAGTTTATCATGAGTTTTGGTTTGAGGAAGCCAC
  AAATTTAGCAACCAAACTTGACATTCAAATGAAACTCCCAGGGAAGTTCCGGAGAGCCCAGCAAGGTAAC
  CTGGAATCTCAGCTAACCTCTGAGAGTTATTATAAAGACACTCTCAGTGTTCCAACAGTAGAGCACATTA
  TTCAGGAACTTAAAGATATTTTCTCTGAACAGCACCTCAAAGCTCTGAAATGCCTATCTCTGGTACCCTC
  AGTGATGGGGCAGCTCAAATTTAACACATCAGAAGAGCACCATGCTGACATGTACAGAAGTGACCTACCC
  AACCCTGACACGCTCTCTGCCGAGCTTCACTGTTGGAGAATCAAGTGGAAACACAGAGGGAAAGATATCG
  AGCTCCCGTCCACCATTTATGAAGCCCTCCATCTTCCTGACATCAAGTTTTTCCCTAATGTATATGCTTT
  GCTGAAGGTCCTGTGTATTCTCCCTGTGATGAAAGTTGAGAATGAGCGCTATGAAAATGGACGGAAGCGT
  CTCAAAGCATACCTGCGGAACACTCTGACAGACCAGAGGTCAAGCAATTTGGCTTTGCTTAACATAAATT
  TTGATATAAAACATGATTTAGATTTAATGGTGGACACATACATCAAACTCTATACAAATAAGTCAGAGCT
  TCCTACAATTAATTCAGAAACCATTGAAAATACCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028410
• Insert Size: 2277 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028410.1, NP_082686.1
• RefSeq Size: 3478 bp
• RefSeq ORF: 2277 bp
• Locus ID: 72981
• UniProt ID: Q9CUX1
• Cytogenetics: 7 E1
• Gene Summary: Upstream regulator of interferon-induced serine/threonine protein kinase R (PKR). May block the PKR-inhibitory function of DNAJC3, resulting in restoration of kinase activity and suppression of cell growth.[UniProtKB/Swiss-Prot Function]