Pou2f1 (NM_198933) Mouse Untagged Clone

CAT#: MC221373

Pou2f1 (untagged) - Mouse POU domain, class 2, transcription factor 1 (Pou2f1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pou2f1
• Synonyms: 2810482H01Rik; NF-A1; Oct-1; Oct1; Otf-1; Otf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221373 representing NM_198933
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGACTGCAGTGACTGTGTTCTAGACTCAAGAATGAATAATCCATCAGAAACCAATAAATCATCTA
  TGGAGAGTGAAGATGCCAGCACAGGCACACAAACCAATGGTCTGGACTTTCAGAAACAGCCCGTGCCCGT
  TGGAGGAGCGATCTCCACAGCCCAGGCCCAGGCCTTCCTCGGACATCTTCACCAGGTCCAGCTAGCTGGG
  ACAAGTTTACAGGCTGCTGCTCAGTCTTTAAATGTACAGTCTAAATCCAGTGAAGAGTCGGGAGATTCGC
  AGCAGTCGAGCCAGCCTTCTTCCCAGCCGCCTTCAGTGCAGTCAGCCATTCCCCAGACCCAGCTAATGCT
  GGCTGGGGGACAGATAACTGGGCTCACGTTGACCCCAGCCCAGCAACAGTTACTGCTACAGCAGGCGCAG
  GCCCAGGCCCAGCTCCTGGCCGCTGCAGTGCAGCAACACTCCGCCAGCCAACAGCACAGTGCTGCTGGGG
  CCACCATCTCAGCCTCCGCCGCCACACCCATGACGCAGATCCCCCTGTCTCAGCCCATACAGATTGCACA
  GGATCTTCAACAATTGCAACAGCTTCAGCAGCAAAATCTCAACTTGCAACAGTTTGTGTTGGTGCACCCA
  ACCACCAACCTGCAACCAGCACAGTTTATCATCTCACAGACCCCCCAGGGCCAGCAGGGTCTCCTGCAAG
  CGCAAAATCTTTTAACGCAACTACCTCAGCAAAGCCAAGCCAACCTCCTACAGCCACAGCCAAGCATCAC
  CCTCACGTCCCAGCCTACCACCCCAACTCGCACAATAGCAGCAGCCTCAGTTCAGACACTTCCACAGAGC
  CAGTCAACACCAAAGCGAATTGACACTCCCAGCTTGGAGGAGCCCAGTGACCTTGAGGAGCTTGAGCAGT
  TTGCCAAGACTTTCAAACAAAGACGAATCAAACTTGGATTCACTCAGGGTGATGTTGGGCTCGCTATGGG
  GAAATTATATGGAAATGACTTCAGCCAAACCACCATCTCTCGCTTTGAAGCCTTGAACCTCAGCTTTAAG
  AACATGTGCAAGTTAAAGCCCCTTTTAGAGAAGTGGCTAAATGATGCAGAGAACCTCTCATCTGATTCTA
  CAGCATCTAGCCCAAGTGCTTTGAATTCTCCAGGATTGGGGGCTGAGGGCTTGAATCGTAGGAGGAAAAA
  ACGCACCAGCATAGAGACCAACATCCGTGTGGCCTTAGAGAAGAGTTTCATGGAGAATCAAAAGCCTACC
  TCGGAAGACATCACCTTGATTGCTGAACAGCTCAATATGGAAAAGGAGGTGATTCGTGTTTGGTTTTGTA
  ACCGCCGCCAGAAGGAGAAAAGAATCAACCCGCCCAGCAGTGGTGGGACCAGCAGCTCACCTATCAAAGC
  AATTTTCCCCAGCCCAGCCTCATTGGTGGCAACCACTCCAAGCCTTGTGACAAGCAGTACGGCAACTACC
  CTCACAGTCAACCCTGTCCTCCCTTTAACCAGTGCTGCTGTGACTAATCTCTCTCTTACAGGCACTACAG
  ACTCGACGTCCAACAACAACACGGCCACGGTGATTTCCACAGCACCCCCTGCTTCCTCAGCAGTCACATC
  CCCTTCCTTGAGTCCCTCTCCCTCTGCCTCGGCCTCCACCTCAGAGGCCTCCAGTGCCAGTGAGACCAAC
  ACGACACAGACCACCTCCACGCCTCTTCCCTCCCCTCTCGGAGCCAGCCAGGTGATGGTGACCACGCCCG
  GCTTACAGACAGCAGCCGCCGCTCTCCAAGGAGCGGCACAGTTGCCAGCAAACGCCAGTCTTGCTGCTAT
  GGCTGCTGCTGCGGGACTCAGCCCAGGCCTCATGGCACCCTCACAGTTTGCTGCTGGAGGTGCCTTACTC
  AGTCTCAGTCCGGGGACTCTGGGCAGTGCTCTCAGCCCAGCCCTAATGAGCAACAGTACACTGGCAACGA
  TTCAAGCTCTTGCTTCTAGTGGCTCTCTTCCAATAACGTCTCTGGATGCAACTGGGAACCTGGTATTTGC
  CAATGCAGGAGGAGCCCCGAACATCGTGACTGCACCTCTGTTCCTGAACCCTCAGAACCTCTCTCTGCTC
  ACCAGCAACCCAGTAAGCTTGGTTTCTGCCGCTGCAGCCTCCACAGGGAACTCTGCACCTACAGCCAGCC
  TTCATGCCTCCTCCACCTCAACTGAGTCCATCCAGAGCTCTCTGTTCACAGTCGCCTCTGCCAGTGGGCC
  TGCTTCCACCACCACAGCTGCCTCCAAGGCACAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_198933
• Insert Size: 2277 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198933.3, NP_945151.2
• RefSeq Size: 12978 bp
• RefSeq ORF: 2277 bp
• Locus ID: 18986
• UniProt ID: P25425
• Cytogenetics: 1 73.21 cM
• Gene Summary: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate 5' terminal exon and lacks an in-frame exon in the coding region, compared to variant 1. It encodes isoform L, which is shorter and has a distinct N-terminus, compared to isoform A. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.