Ppm1e (NM_177167) Mouse Untagged Clone

CAT#: MC221341

Ppm1e (untagged) - Mouse protein phosphatase 1E (PP2C domain containing) (Ppm1e), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ppm1e
• Synonyms: AW049266; B930008A12Rik; mKIAA1072; POPX1; PP2CH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221341 representing NM_177167
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCTGCATCCCTGAGGAGAAAACTTACCGGCGCTTCCTGGAGCTGTTCCTGGGCGAGTTCCGCG
  GACCGTGCGGTGGCGGCGAGCCAGAGCCCGAACCCGAATCCGAGCCCGAGCCCGAGCCCGAAGCCGAGCT
  AGTCGCGGCTGAGGCGGCCGAGGCTTCGGGGGAGGAACCGGGGGAGGACGCTGCGACGGTAGAGGCGACC
  GAGGAGGGGGAGCAGGACCAAGACCCCGAGCCCGAAGACGAGGCGGTGGAGGAGGAGACGGCGACGGAGG
  GCGAGGAGGAGGAAGAGGAGGAAGCGGCTGCCCCGGGGCACTCGGCCGTGCCGCCACCGCCGCAGCCCCA
  GCTGCCACCGCTGCCCCCGCTCCCACGGCCGCTGTCGGAGCGCATCACCCGAGAGGAGGTGGAGGGCGAG
  AGCCTGGACCTGTGCCTGCAGCAGCTCTACAAATATAACTGCCCTTCCTTTCTGGCTGCTGCTTTAGCCA
  GAGCCACATCAGATGAAGTCCTCCAGAGTGACCTTTCTGCTCACTGTATCCCAAAGGAAACGGACGGCAC
  AGAAGGGACTGTGGAAATTGAGACAGTGAAATTGGCCCGTTCTGTCTTCAGCAAACTACATGAGATCTGC
  TGCAGTTGGGTGAAGGACTTCCCTCTGCGCAGGAGGCCTCAGATTTATTATGAGACGTCAATTCATGCCA
  TCAAGAACATGCGAAGGAAAATGGAGGACAAACACGTCTGCATTCCTGACTTTAACATGCTCTTCAACCT
  AGAGGACCAGGAGGAACAAGCATACTTTGCAGTGTTTGATGGCCATGGGGGAGTGGACGCTGCTATTTAT
  GCCTCCGTTCACCTACACGTTAACTTAGTGCGCCAGGAGATGTTTCCCCATGATCCTGCTGAGGCTCTCT
  GCCGGGCCTTCCGTGTCACAGATGAGCGGTTTGTGCAGAAGGCAGCCAGGGAGAGCTTACGGTGTGGGAC
  CACAGGAGTAGTGACTTTTATCAGAGGCAACATGCTCCACGTGGCATGGGTGGGCGATTCCCAGGTCATG
  CTGGTAAGAAAGGGGCAAGCTGTTGAACTAATGAAACCACACAAGCCAGACAGAGAGGATGAAAAGCAGC
  GAATTGAGGCCCTTGGAGGCTGTGTGGTTTGGTTTGGCGCCTGGAGGGTGAATGGAAGCCTGTCCGTCTC
  CAGAGCCATCGGAGATGCTGAGCACAAGCCGTATATCTGTGGAGACGCAGACTCGGCCTCCACTGTTTTG
  GATGGGACTGAAGACTACCTCATTCTGGCCTGTGACGGCTTCTATGACACCGTGAACCCTGACGAGGCAG
  TGAAGGTTGTGTCTGACCACTTGAAAGAGAATAACGGAGACAGCAGCATGGTTGCCCACAAATTAGTAGC
  GTCGGCTCGTGATGCGGGGTCAAGTGATAACATCACTGTTATTGTGGTATTCCTGAGGGACATGAACAAG
  GCTGTAAATGTTAGTGAGGAGTCAGAGTGGACAGAGAACTCTTTTCAAGGAGGGCAAGAAGATGGTGGGG
  ATGATAAGGAGACTCATGGAGAGTGCAAGCGCCCTTGGCCACAGCACCAGTGCTCAGCACCCGCCGATCT
  AGGCTATGAGGGGCGAGTGGATTCATTCACTGATAGAACTAGCCTCAGCCCAGGGCCCCAAATCAACGTG
  CTGGAAGATCCAGACTACCTAGATCTCACACAAATAGAAGCAAGCAAACCTCACAGTACCCAATTTTTGC
  CACCAGTTGAGATGATTGGTCCTGGTGCACCAAAGAAAGATCTTAATGAGCTAATAATGGAGGAGAGATC
  AGTCAAGTCATCATTGCCTGAACGGAGTGGTGCTGGAGAGCCTCGGGTTTCTTTTAATTTGGGTTCAACA
  GGGCAACAGATATGCAGAATGGAGAACTTGTCTCCTGTCAGTTCTGGGTTGGAAAATGAACAGTTCAAAT
  CCCGGGGGAAGACAGCGTCTAGGTTGTATCATTTACGCCACCACTACTCCAAAAGGCAGCGTGGATTCAG
  GTTTAATCCAAAGTTTTACTCGTTTCTGTCTGCTCGAGAGCCTTCTCACAAAATAGGCATTAGCCTCTCC
  TCACTTACTCGAAGTGGGAAGAGAAACAAGATGTTAAGAAGTTCTTTGCCATGGAGGGAAAATAGTTGGG
  AAGGATATAGTGGAAATGTGAAGATCAGAAAGCGTAATGATATTCCATGCCCAGACTTTCCCTGGAGCTA
  TAAAATTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177167
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC138900, AAI38901
• RefSeq Size: 2781 bp
• RefSeq ORF: 2250 bp
• Locus ID: 320472
• UniProt ID: Q80TL0
• Cytogenetics: 11 C
• Gene Summary: Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2. Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42 (By similarity). Dephosphorylates PRKAA2.[UniProtKB/Swiss-Prot Function]