Akap5 (NM_001101471) Mouse Untagged Clone

CAT#: MC221335

Akap5 (untagged) - Mouse A kinase (PRKA) anchor protein 5 (Akap5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Akap5
• Synonyms: 3526401B18Rik; AKAP-5; AKAP 150; AKAP150; BB098886; Gm258; P150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221335 representing NM_001101471
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCAGCGTTTCTGAGATTCAGGTAGAGACGAAGGACGAGAAGGGACCAGTAGCAGCCAGTCCTC
  AGAAAGAGAGGCAGGAGAGAAAAACAGCCACACTCTGCTTCAAGAGAAGGAAGAAAGCGAACAAGACAAA
  ACCCAAAGCCGGTTCCAGGACTGCTGAGGAGACAAAGAAACATACCCCCGAAGCTGGGGGCTCTGGTCAA
  CGGCAACCAGCGGGTGCCTGGGCCTCCATCAAAGGCCTTGTGACACACAGGAAAAGGTCCGAGCCTGCAA
  AGAAGCAGAAGCCGCCTGAGGCAGAGGTGCAGCCCGAGGATGGGGCTCTTCCTAAGAAAAAGGCAAAATC
  CAGACTTAAGTTTCCTTGCCTAAGATTCTCAAGAGGGGCAAAGAGAAGTCGTCATTCCAAACTCACAGAA
  GATTCAGGCTACGTCAGAGTCCAGGGAGAGGCAGACGATTTGGAGATAAAAGCCCAGACCCAGCCAGATG
  ACCAGGCAATCCAGGCTGGGTCTACCCAGGGCCTACAGGAAGGTGTGTTGGTGAGAGATGGTAAGAAGAG
  CCAAGAATCACACATAAGCAACAGCGTCACCTCGGGAGAGAATGTGATCGCCATAGAACTGGAATTAGAA
  AACAAATCTTCTGCTATCCAGATGGGAACTCCTGAGCTGGAAAAGGAGACTAAGGTGATCACGGAAAAGC
  CAAGTGTGCAGACGCAGCGAGCAAGTCTACTTGAGAGTTCAGCAGCAGGCAGCCCTCGGTCAGTGACTTC
  TGCTGCTCCTCCATCACCAGCAACCACACATCAACATAGTCTGGAAGAACCCAGTAATGGCATCCGGGAA
  AGTGCCCCAAGTGGGAAAGATGACAGGAGAAAGACTGCTGCTGAAGAAAAGAAATCAGGAGAGACTGCAC
  TGGGCCAGGCAGAGGAAGCTGCAGTTGGCCAGGCAGACAAAAGGGCACTAAGCCAGGCCGGAGAAGCTAC
  AGCGGGCCACCCAGAGGAAGCTACTGTGATCCAGGCAGAGAGTCAGGCAAAGGAAGGGAAACTGAGCCAG
  GCAGAGGAAACTACAGTGGCCCAGGCAAAGGAAACTGTATTGAGTCAGGCAAAGGAAGGTGAACTGAGCC
  AGGCAAAGAAAGCTACAGTTGGCCAGGCAGAGGAAGCTACAATTGACCACACAGAGAAAGTCACAGTGGA
  TCAGGCAGAGGAAACCACAGTGGGTCAGGCAGAAGAAGCTACAGTTGGTCAGGCAGGGGAAGCTATATTG
  AGCCAAGCAAAGGAAGCTACAGTTGTTGGTCAGGCAGAGGAAGCTACAGTTGATCGGGCAGAGGAAGCTA
  CTGTTGGTCAGGCAGAGGAAGCTACAGTTGGCCACACAGAGAAAGTCACAGTGGATCAGGCAGAGGAAGC
  CACGGTGGGTCAGGCAGAGGAAGCTACTGTTGGTCAGGCAGAGGAAGCTACAGTTGATTGGGCAGAAAAA
  CCCACAGTTGGTCAGGCAGAGGAAGCCACAGTGGGTCAGGCAGAGGAAGCTACAGTTGGCCACACAGAGA
  AAGTCACAGTGGATCAGGCAGAGGAAGCAACAGTGGGTCAGGCAGAGGAAGCTACAGTTGGCCACACAGA
  GAAAGTCACAGTGGATCATGCAGAGGAAGCCACAGTGGGTCAGGCAGAGGAAGCTACAGTTGGACAGGCA
  GAGAAAGTCACAGTGGATCATGCAGAGGAAGCCACAGTGGGTCAGGCAGAGGAAGCTACAGTTGGACAGG
  CAGAGAAAGTCACAGTGGATCATGCAGAGGAAGCCACAGTGGGTCAGGCAGAGGAAGCTACAGTTGGACA
  GGCAGAGAAAGTCACAGTGGATCAGGCAGAGGAACCCACAGTGGATCAGGCTGAGGAAGCTATATCGAGC
  CATGCACCAGATCTGAAAGAAAATGGAATTGATACAGAGAAACCGAGATCAGAAGAAAGCAAACGAATGG
  AGCCAATTGCTATTATCATTACTGACACTGAAATCAGTGAATTTGATGTTAAGAAGTCTAAAAATGTTCC
  TAAGCAATTCCTAATTTCAATGGAAAACGAGCAAGTGGGGGTTTTTGCTAATGATAGTGATTTCGAGGGG
  AGAACTTCAGAACAGTATGAAACACTCTTAATAGAAACAGCATCTTCTCTCGTCAAGAATGCTATCGAGT
  TGTCTGTAGAACAGCTGGTTAATGAAATGGTTTCCGAGGATAATCAAATAAATACGCTGTTCCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001101471
• Insert Size: 2238 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001101471.1, NP_001094941.1
• RefSeq Size: 6685 bp
• RefSeq ORF: 2238 bp
• Locus ID: 238276
• UniProt ID: D3YVF0
• Cytogenetics: 12 C3
• Gene Summary: May anchor the PKA kinase to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors.[UniProtKB/Swiss-Prot Function]