Mark4 (NM_172279) Mouse Untagged Clone

CAT#: MC221332

Mark4 (untagged) - Mouse MAP/microtubule affinity-regulating kinase 4 (Mark4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mark4
• Synonyms: 2410090P21Rik; C79806; Markl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221332 representing NM_172279
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCGAGGACGGCGCTGGCCCCGGGCAACGATCGAAACTCGGACACGCATGGCACATTGGGCAGTG
  GACGATCTTCGGACAAAGGGCCGTCCTGGTCCAGCCGTTCCCTGGGTGCCCGTTGCCGGAACTCTATCGC
  TTCCTGCCCTGAGGAACAACCCCATGTGGGCAACTATAGGCTGCTAAGGACCATCGGGAAGGGCAACTTC
  GCCAAAGTCAAGCTGGCTCGGCATATCCTCACGGGCCGGGAGGTCGCTATTAAGATCATTGATAAGACCC
  AGCTGAACCCCAGTAGCTTGCAGAAGCTGTTCAGAGAAGTCCGAATTATGAAGGGACTCAACCACCCCAA
  CATCGTGAAGCTTTTTGAGGTGATAGAGACGGAGAAGACGCTATACCTGGTGATGGAATACGCTAGCGCA
  GGAGAAGTGTTTGACTACCTCGTGTCGCACGGCCGCATGAAGGAGAAGGAGGCTCGAGCCAAGTTCCGGC
  AGATCGTGTCAGCCGTGCACTACTGTCATCAGAAGAACATTGTACACAGGGATCTAAAGGCTGAAAACCT
  GTTGCTGGATGCCGAGGCCAACATCAAAATCGCCGACTTCGGCTTCAGCAATGAGTTCACGCTGGGCTCC
  AAGCTGGACACCTTCTGTGGGAGCCCCCCATACGCCGCCCCAGAGCTGTTCCAGGGCAAGAAGTATGATG
  GGCCAGAGGTGGACATCTGGAGCCTGGGTGTCATCCTGTACACGCTGGTCAGCGGCTCCCTGCCCTTCGA
  TGGGCACAACCTCAAGGAGCTGCGGGAGCGAGTCCTCAGAGGAAAGTACCGGGTCCCCTTCTACATGTCT
  ACAGACTGCGAGAGCATTCTGCGGAGATTTCTGGTGCTGAACCCCGCAAAACGCTGTACTCTGGAGCAAA
  TCATGAAAGACAAATGGATCAACATCGGCTATGAGGGTGAGGAGCTGAAGCCATACACGGAGCCTGAGGA
  GGACTTCGGGGACACCAAGAGAATTGAGGTGATGGTGGGTATGGGCTACACACGGGAAGAAATCAAAGAG
  GCCTTGACCAACCAGAAGTACAACGAGGTGACCGCCACCTACCTCCTGCTGGGCAGGAAGACTGAGGAGG
  GTGGGGACCGGGGTGCCCCAGGGCTGGCCCTGGCACGGGTGCGGGCGCCCAGCGACACCACCAACGGGAC
  AAGCTCCAGCAAAGGCAGCAGCCACAACAAAGGGCAACGGGCTTCTTCCTCCACCTACCACCGCCAGCGC
  CGTCACAGTGACTTCTGTGGCCCGTCCCCTGCCCCGCTGCACCCGAAGCGCAGCCCAACCAGCACGGGAG
  ACACGGAGCTCAAAGAAGAGCGGATGCCGGGTCGGAAAGCGAGCTGCAGTGCAGTGGGCAGTGGAAGTCG
  AGGCTTGCCCCCCTCCAGCCCCATGGTCAGCAGTGCCCACAACCCCAATAAGGCAGAGATCCCTGAGCGG
  CGGAAGGACAGCACTAGCACCCCTAACAACCTCCCCCCCAGCATGATGACCCGAAGAAACACCTATGTGT
  GCACAGAGCGACCAGGATCTGAACGCCCGTCCTTGTTGCCAAATGGCAAAGAAAATAGCTCCGGTACCTC
  GCGGGTGCCCCCTGCCTCGCCTTCCAGTCATAGCCTGGCTCCCCCGTCAGGCGAGCGGAGCCGCCTGGCT
  CGGGGCTCCACCATCCGCAGCACCTTCCATGGGGGCCAGGTCCGAGACCGGCGGGCAGGGAGCGGGAGTG
  GCGGGGGTGTGCAGAATGGACCCCCAGCCTCACCCACGCTTGCCCACGAGGCCGCACCCCTGCCCTCCGG
  GCGGCCTCGCCCCACCACCAACCTCTTCACCAAGCTGACCTCCAAACTGACCCGAAGGGTCACAGACGAA
  CCTGAGAGAATCGGGGGACCTGAGGTCACAAGTTGCCATCTACCTTGGGATAAAACGGAAACCGCCCCCA
  GGCTGCTCCGATTCCCCTGGAGTGTGAAGCTGACCAGCTCGCGACCTCCTGAGGCCCTGATGGCTGCCCT
  GCGACAGGCCACAGCGGCCGCCCGCTGCCGGTGCCGCCAGCCGCAGCCGTTCCTGCTGGCCTGCCTGCAC
  GGGGGTGCGGGCGGGCCCGAGCCCCTGTCCCATTTCGAAGTGGAGGTGTGCCAGCTGCCCCGGCCCGGCC
  TCAGGGGCGTCCTCTTCCGCCGTGTGGCGGGCACCGCCCTGGCCTTCCGCACCCTCGTCACCCGCATCTC
  CAACGACCTCGAACTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172279
• Insert Size: 2259 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172279.1, NP_758483.1
• RefSeq Size: 2259 bp
• RefSeq ORF: 2259 bp
• Locus ID: 232944
• UniProt ID: Q8CIP4
• Cytogenetics: 7 A3
• Gene Summary: Serine/threonine-protein kinase (By similarity). Phosphorylates the microtubule-associated protein MAPT/TAU (By similarity). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (By similarity). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (By similarity). Required for the initiation of axoneme extension during cilium assembly (By similarity). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (By similarity). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (By similarity). Reduces neuronal cell survival (By similarity). Plays a role in energy homeostasis by regulating satiety and metabolic rate (PubMed:22992738). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (PubMed:24989893). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (By similarity).[UniProtKB/Swiss-Prot Function]