Lin54 (NM_001115010) Mouse Untagged Clone

CAT#: MC221330

Lin54 (untagged) - Mouse lin-54 homolog (C. elegans) (Lin54), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lin54
• Synonyms: AI461788
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221330 representing NM_001115010
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTGGTGCCGGCCGAGGTGAATAGTTTGCTTCCAGACGACATAATGGACACTGCTATAACGTTAG
  TGGATGAGGATAGTATTGAGGCTGTAATTGTGTCATCCCCAATTCCCATGGAGACAGAGCTGGAAGAAAT
  TGTCAACATAAATTCTACCGGTGACTCTACAGCCACGCCCATTTCCACGGAACCAATCACAGTGTACAGT
  AACCACACTAACCAAGTTGCAGTGAATACCACAGTTTCTAAAGCAGATTCTAATACCACAGTGAAACCAG
  CATTTCCAAGTGGCCTTCAAAAACTTGGTGCTCAGACTCCTGTGACTATATCAGCCAATCAGATTATTTT
  AAACAAAGTATCACAGACATCTGATCTTAAACTTGGCAATCAGACCCTTAAACCAGATGGACAGAAGTTA
  ATATTAACAACTTTGGGCAAGTCTGGTTCACCAATTGTTTTAGCACTACCCCATAGCCAACTACCCCAGG
  CTCAGAAAGTTACAGCTCAGGCCCAGCCAGGAGATGCTAAGTTACCACCGCAGCAAATTAAAGTAGTTAC
  CATTGGAGGGAGGCCAGAGGTGAAACCTGTCATTGGTGTCTCAGCACTGACCCCAGGAAGCCAACTGATT
  AATACTACAACTCAGCCCTCTGTGTTACAGACCCAACAGTTAAAAACAGTACAGATTGCTAAGAAGCCTC
  GGACACCAACCTCGGGTCCAGTGATCACGAAGCTGATTTTTGCAAAACCAATTAATAGCAAAGCAGTTAC
  AGGACAGACAACTCAAGCATCTCCACCCGTCGTCACAGGTAGGGTTCTTTCACAGTCTACTCCTGGAACT
  CCATCAAAGACTATAACAATATCAGAAAGTGGTGTGATTGGATCAACTTTAAATTCGACAACACAGACAC
  CAAATAAGATAGCCATCTCACCTTTGAAGTCGCCAAATAAGACAGTGAAATCGGCTGTGCAGACCATCAC
  TGTGGGAGGAATGAGCACCTCACAGTTTAAGACCATCATCCCTCTGGCCACTGCTCCCAATGTCCAGCAG
  ATTCAAGTGCCTGGAAGCAAGTTTCATTATGTCCGACTTGTTACTGCCACAACAGCCAGTAGCTCAGCCC
  AGCCAGTCAGTCAGAGCCCCAGTGTAAACACACAGCCTCTCCAGCAAGCAAAACCAGTGGTTGTTAATAC
  CACCCCTGTACGGATGTCAGTTCCATTTGTCCAAGCTCAGGCCGTTAAGCAAGTTGTTCCAAAACCAATC
  AATTCAACTTCACAAATTGTAACTACCAGCCAGCCACAGCAGCGGCTCATCATGCCTGCCACACCACTGC
  CTCAGATCCAGCCCAACCTCACTAACCTGCCACCAGGTACTGTGCTGGCACCAGCTCCAGGAACAGGGAA
  TGTGGGCTATGCAGTGCTTCCAGCTCAGTATGTTACTCAGTTACAGCAGTCTTCATATGTGTCTATAGCA
  AGCAACTCTAATTTTACTGGAACATCTGGCATCCAGACACAGGCAAGGCTTCCATTCAATGGCATAATTC
  CATCAGAGTCTACCAGTCGGCCCAGAAAACCCTGTAACTGTACAAAATCACTGTGTTTGAAATTATACTG
  TGATTGCTTTGCAAATGGTGAATTCTGCAACAACTGCAACTGTACCAATTGTTACAACAATTTAGAGCAC
  GAGAATGAGAGACAAAAGGCAATCAAGGCATGCCTTGATAGAAATCCGGAAGCCTTCAAGCCAAAGATAG
  GAAAAGGAAAAGAGGGGGAGTCGGATCGGCGGCACAGCAAAGGCTGCAACTGCAAACGCTCGGGGTGTCT
  TAAAAACTACTGCGAGTGCTATGAGGCAAAAATAATGTGTTCTTCTATATGCAAATGCATCGGCTGTAAG
  AATTTTGAAGAAAGCCCAGAACGGAAGACATTGATGCACTTAGCAGATGCGGCTGAAGTAAGGGTCCAGC
  AGCAGACAGCAGCGAAGACCAAGCTGTCCTCTCAGATCTCAGACCTCCTTACAAGGCCAACCCCAGCTCT
  CAACAGTGCAGGAGGGAAATTGCCGTTTACATTTGTAACTAAAGAAGTAGCTGAAGCCACGTGTAATTGC
  CTTCTTGCCCAGGCAGAGCAGGCAGACAAGAAGGGGAAATCAAAGGCGGCAGCAGAGCGGATGATACTTG
  AGGAATTTGGACGATGTTTGATGAGCGTCATCAACTCTGCAGGAAAAGCCAAAAGTGACCCCTGTGCCAT
  GCACTGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001115010
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001115010.1, NP_001108482.1
• RefSeq Size: 4596 bp
• RefSeq ORF: 2250 bp
• Locus ID: 231506
• UniProt ID: Q571G4
• Cytogenetics: 5 E4
• Gene Summary: Component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context. In G0 phase, the complex binds to more than 800 promoters and is required for repression of E2F target genes. In S phase, the complex selectively binds to the promoters of G2/M genes whose products are required for mitosis and participates in their cell cycle dependent activation. In the complex, acts as a DNA-binding protein that binds the promoter of CDK1 in a sequence-specific manner. Specifically recognizes the consensus motif 5'-TTYRAA-3' in target DNA.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Variants 1 and 2 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.