Stim2 (NM_001081103) Mouse Untagged Clone

CAT#: MC221321

Stim2 (untagged) - Mouse stromal interaction molecule 2 (Stim2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Stim2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221321 representing NM_001081103
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTCTTCGGGTTGTTGGTGGCCGGCGTGGCGGACGGATGCGATCTGGTGCCCCGGCACCTCCGCG
  GGCGGCGCGCGTCGGGATCGGCCGGAGCGGCGGCGTCTCCTTCGGCTGCGGCTGCGGGCGAGCGCCAGGC
  GCTGCTGACAGATCCCTGTATGTCGCTGAGTCCACCTTGCTTCACTGAAGAAGACAGGTTTAGCTTGGAA
  GCACTTCAGACAATACACAAACAGATGGACGACGACAAGGACGGCGGGATCGAAGTGGACGAGAGTGATG
  AGTTTATCAGAGAAGATATGAAATATAAAGATGCTACGAATAAACATAGTCACCTGCACAGAGAAGATAA
  GCACATAACTGTTGAGGATTTGTGGAAACAGTGGAAAACATCAGAAGTTCACAATTGGACACTTGAGGAT
  ACCCTGCAGTGGTTAATAGAATTTGTTGAACTGCCACAATATGAGAAGAATTTTAGGGATAATAATGTGA
  AGGGAACAACACTCCCCAGGATAGCAGTTCATGAAACTTCATTTATGATTTCCCAGTTGAAAATCAGCGA
  CCGAAGTCACAGACAGAAACTCCAACTCAAAGCCCTGGATGTGGTTCTGTTTGGGCCTCTGACACGCCCA
  CCTCATAACTGGATGAAGGATTTTATTCTCACAATTTCCATAGTAATTGGTGTTGGGGGTTGTTGGTTTG
  CTTATACACAAAATAAGACATCAAAGGAACATGTTGCAAAAATGATGAAAGACTTAGAGAGTCTGCAGAC
  TGCAGAGCAGAGTCTCATGGACTTACAAGAGAGACTTGAAAAGGCACAGGAAGAAAACAGAACTGTTGCT
  GTAGAAAAGCAAAATCTGGAACGGAAAATGATGGATGAAATCAACTATGCCAAGGAGGAGGCCTGTCGGC
  TGCGGGAGCTGAGGGAGGGCGCAGAGTGTGAGCTGAGCAGGCGCCAGTATGCAGAGCAGGAACTGGAGCA
  GGTCCGCATGGCTCTAAAAAAGGCCGAAAAGGAGTTTGAACTGAGAAGCAGCTGGTCTGTCCCTGACGCA
  CTACAGAAATGGCTTCAGCTAACACACGAAGTTGAAGTACAGTACTACAATATTAAGAGGCAAAATGCTG
  AGATGCAGCTAGCCATCGCTAAGGACGAGGCAGAAAAAATTAAAAAGAAGAGAAGCACAGTCTTTGGGAC
  CCTGCACGTTGCACACAGCTCCTCCCTGGACGAAGTAGACCACAAGATTCTGGAAGCCAAGAAAGCCCTC
  TCTGAGCTGACCACGTGCTTGCGAGAACGGCTTTTTCGCTGGCAGCAGATTGAGAAGATCTGTGGCTTTC
  AGATAGCTCACAACTCTGGGCTCCCCAGTCTCACCTCCTCTCTGTACTCTGACCACAGCTGGGTGGTGAT
  GCCTAGAGTCTCCATTCCACCCTACCCTATTGCTGGAGGAGTTGATGACCTCGATGAAGACACACCCCCA
  ATCGTGCCACAGTTTCCAGGGACCGTGGCTAAACCTGCAGGATCTTTAGCCAGAAGCAGTAGTTTATGCC
  GCTCTCGTCGCAGCATCGTGCCATCCTCCCCACAGTCTCAGCGAGCTCAGCTTCCTGCTCATGCTCCTCT
  GGCAGCCCACCCTCGGCACCCTCACCATCCGCAGCATCCCCAGCACTCGTTGCCTTCCCCAGATCCAGAC
  ATCCTGTCTGTGTCAAGTTGCCCTGCTCTGTATCGGAACGAAGAGGAGGAGGAGGCTATCTACTTCACTG
  CTGAGAAACAATGGGAAGTGCCAGACACAGCTTCAGAATGTGACTCCTTAAACTCTTCCAGTGGGAGAAA
  ACCGTCTCCCCCTTCAAGCCTTGAGATGTACCAAACATTGTCTTCCCGAAAAATCTCAAGAGACGAGCTT
  TCCCTGGAGGACTCTTCCAGGGGGGAGTCACCCGTGACAGCAGATGTCTCCCGGGGCTCCCCTGAGTGTG
  TGGGTCTGACGGAGACCAAGAGCATGATCTTCAGCCCTGCAAGCAGAGTGTACAATGGCATCCTGGAGAA
  ATCCTGCAGCATGCACCAGCTCTCCAGCGGCATCCCGGTGCCTCATCCCCGACACACATCGTGCTCCTCA
  GCCGGCAATGATAGCAAGCCAGTTCAGGAAGCCTCGAATGTTTCCAGAGTAAGCAGCATCCCACATGACC
  TCTGTCATAATGGTGAGAAAAGCAAAAAGCCATCCAAAATCAAAAGCCTTTTCAAGAAGAAGTCTAAGTG
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081103
• Insert Size: 2241 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081103.2, NP_001074572.2
• RefSeq Size: 4930 bp
• RefSeq ORF: 2241 bp
• Locus ID: 116873
• UniProt ID: P83093
• Cytogenetics: 5 29.69 cM
• Gene Summary: Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Functions as a highly sensitive Ca(2+) sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca(2+)-influx. Regulates basal cytosolic and endoplasmic reticulum Ca(2+) concentrations. Upon mild variations of the endoplasmic reticulum Ca(2+) concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca(2+) release-activated Ca(2+) (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca(2+) influx (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to represent an alternate splicing pattern and to use a downstream start codon. PMID:11463338 provides experimental evidence for the use of the downstream start codon, but no data is available regarding the use of the upstream start codon.