Lima1 (NM_001113545) Mouse Untagged Clone

CAT#: MC221301

Lima1 (untagged) - Mouse LIM domain and actin binding 1 (Lima1), transcript variant a, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lima1
• Synonyms: Eplin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221301 representing NM_001113545
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATCGACTCCATTTAATAGACGCCAGTGGACTTCCCTGTCATTGAGAGTAACAGCCAAAGAGCTTT
  CTCTTGTCAACAAGAACAAGTCATCCGCAATTGTGGAAATATTCTCCAAGTACCAGAAGGCTGCTGAAGA
  AGCCAACATGGAAAGGAAGAAAAATAACCCTGAAAGTCTGCCCCAGCACTTTAGAAGAGGGACCCTGTCT
  GTGTTAAAGAAGAAGTGGGAGAACCCGGTGGCTGGGGCAGAATTCCACACAGACTCACTGCCAAACAGCA
  GCAGTGAGGGTGGGCACACAGCGGACTACCCTCCTGCTGAAGTGACGGACAAGCCTGCTCCTGGAGTCAG
  AGCTGACCGGGAAGAGCACACCCAGCCCAAACCTAGATTTGGATCTCGTCCGGAAGCAGTTATCCAGAGC
  CGGTATCCTCGCTCAGAGAACAGCCACGATTTTAAAGCCCAGGCCACGGAGAGCCAAAAAATGGAAAACT
  GTCTGGGAGATTCCAGGCATGAAGCAGAGAAGCCAGAGACGAGCGAAAACACAGAAACTTCAGGCAAAAT
  AGAGAAATACAACGTTCCACTGAATAGACTGAAGATGATGTTTGAGAAAGGTGAACACAACCAAACCAAG
  AGTCTCTGGACCCAAAGCCGAAATGCGGGTGGAAGGAGGCTCTCTGAAAACAACTGTTCCCTGGATGACT
  GGGAAATAGGTGCGGGTCATTTGTCTTCCTCTGCATTCAACTCGGAGAAAAACGAGAGTAAGAGGAATCT
  GGAGCTGCCACGCCTCTCAGAAACCTCCATAAAGGACCGCATGGCCAAGTACCAGGCTGCAGTGTCCAAG
  CAGAGCAGCCCAGCCAGCTACACAAATGAGCTGAAAACCAGTGAAAGCAAAACTCATAAATGGGAACAGA
  AGGAGAATGTGCCCCCAGGTCCCGAGGCCTGCAGCGTCCATCAGGAAGGAAGCAAGGTTTCTACAACTGA
  GAATAGTCTAGTGGCCCTCTCTGTCCCTGCTGAAGATGACACCTGTAACTCCCAGGTGAAGAGCGAGGCC
  CAGCAGCCTATGCACCCTAAGCCGCTGAGTCCTGACGCCAGGACCTCCAGTCTTCCGGAAAGTTCTCCTT
  CCAAAACAGCGAAGAAGTTTCAGGCGCCGGCAAAGGAGAGCTGCGTGGAGTGTCAGAAGACGGTGTACCC
  CATGGAACGGCTCCTGGCCAACCAGCAGGTGTTTCACATCAGCTGTTTCCGATGCTCCTACTGCAACAAC
  AAGCTCAGTCTAGGAACATATGCATCCTTACATGGACGAATCTACTGTAAGCCTCACTTCAATCAACTCT
  TCAAGTCCAAAGGCAACTATGACGAGGGCTTTGGGCACAAGCAGCATAAGGATCTGTGGGCAAGCAAGAG
  CGACAATGAGGAGACTTTGGGGAGACCAGCCCAGCCGCCTAATGCAGGGGAGAGCCCCCATAGCCCGGGT
  GTAGAAGATGCCCCCATCGCCAAGGTCGGCGTGCTGGCGGCAAGTATGGAAGCCAAGGCCTCTTCTCAGA
  GGGAGAGGGAGGATAAGCCTGCGGAGACCAAGAAGCTGAGGATCGCCTGGCCTCCGCCAGCCGAGCTGGG
  CGGTTCCGGAAGTGCCCTGGAGGAAGGGATCAAAGTATCGAAGCCCAAGTGGCCTCCGGAGGATGACGTC
  TGCAAGACGGAGGCCCCGGAGGATGTAGATCTGGACCTGAAGAAGCTGCGGCGGTCTTCGTCCCTGAAGG
  AGAGGAGCCGCCCGTTTACGGTAGCAGCTTCCTTTCGAACCTCTTCCATCAAGAGCCCCAAGGCCTCTTC
  GCCATCTCTCAGGAAAGGTTGGAGCGAGTCCGAGCAGAGTGAGGAGTTTGGAGGAGGGATAGCGACGATG
  GAAAGGAAACAAACGGAAAATGCCAGACCCTCTGGGGAGAAAGAGAATGTGGGAAAATCACGCTGGCAGG
  GCGAGGAAGTGCCGAGGAGCAAGGACCGTAGTTCTTTTGAGCTGGAGAGTGAGAATTTTATGGAAAATGG
  AGCAAACATAGCTGAAGATGACAACCATGTCCACGCACAGCAGTCCCCGTTAGAACCCGAGGCTCCGGGT
  TGGTCTGGCTTTGTAGACACCACGGCTGCTAAAGAATTCACTACCCAGAATCAAAAATCCCAAGATGTCG
  GGTTCTGGGAGGGAGAAGTGGTCCGAGAGCTGTCTGTGGAGGAACAGATAAAGAGAAACCGGTACTATGA
  TGAGGACGAGGATGAAGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001113545
• Insert Size: 2262 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113545.1, NP_001107017.1
• RefSeq Size: 4245 bp
• RefSeq ORF: 2262 bp
• Locus ID: 65970
• UniProt ID: Q9ERG0
• Cytogenetics: 15 56.13 cM
• Gene Summary: Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments (By similarity). Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake (PubMed:29880681).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (a) represents the longer transcript and encodes the longer isoform (a).