Stat4 (NM_011487) Mouse Untagged Clone

CAT#: MC221295

Stat4 (untagged) - Mouse signal transducer and activator of transcription 4 (Stat4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Stat4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221295 representing NM_011487
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCAGTGGAATCAAGTCCAACAATTAGAAATCAAGTTTTTGGAGCAAGTAGATCAGTTCTATGATG
  ACAACTTTCCTATGGAAATCCGGCATCTGCTAGCTCAGTGGATTGAGACTCAAGACTGGGAAGTAGCTTC
  TAACAATGAAACTATGGCAACAATTCTGCTTCAAAACTTACTAATACAATTGGATGAACAGTTGGGGCGT
  GTTTCCAAAGAAAAAAATCTGCTATTGATTCACAATCTAAAGAGAATTAGAAAAGTTCTTCAGGGCAAGT
  TTCATGGAAATCCAATGCATGTAGCTGTGGTAATTTCAAATTGCTTAAGGGAAGAGAGGAGAATATTGGC
  TGCAGCCAACATGCCTATCCAGGGACCTCTGGAGAAATCCTTACAGAGTTCTTCAGTTTCTGAAAGACAA
  AGGAATGTGGAACACAAAGTGTCTGCCATTAAAAACAGTGTGCAGATGACAGAACAAGATACCAAATACT
  TAGAAGACCTGCAAGATGAGTTTGACTACAGGTATAAAACAATTCAGACAATGGATCAGGGTGACAAAAA
  CAGTATCCTGGTGAACCAGGAAGTTTTGACGCTGCAAGAAATGCTTAATAGTCTGGACTTCAAGAGAAAG
  GAAGCACTCAGTAAGATGACGCAGATAGTGAACGAGACAGACCTGCTCATGAACAGCATGCTTCTAGAAG
  AGCTGCAGGACTGGAAAAAGCGGCAGCAGATTGCCTGCATTGGTGGCCCGCTCCACAATGGGCTGGACCA
  GCTTCAGAACTGCTTTACCCTACTGGCAGAGAGTCTTTTCCAACTCAGACAGCAACTGGAGAAACTACAG
  GAGCAATCTACTAAAATGACCTATGAAGGGGATCCCATCCCTGCTCAAAGAGCACACCTCCTGGAAAGAG
  CTACCTTCCTGATCTACAACCTTTTCAAGAACTCATTTGTGGTCGAGCGACAGCCATGCATGCCAACGCA
  CCCTCAGAGGCCGATGGTACTTAAAACCCTCATTCAGTTCACTGTAAAACTGAGATTACTAATAAAATTG
  CCGGAACTAAACTATCAGGTGAAAGTAAAGGCGTCCATTGACAAGAATGTTTCAACTCTAAGCAATAGAA
  GATTTGTGCTTTGTGGAACTCACGTCAAAGCTATGTCCAGTGAGGAATCTTCCAATGGGAGCCTCTCAGT
  GGAGTTTAGACATTTGCAACCCAAGGAGATGAAGTGCAGTACTGGGAGTAAAGGAAACGAGGGCTGCCAC
  ATGGTGACAGAGGAGTTGCATTCCATAACCTTTGAGACCCAGATCTGCCTCTATGGCCTCACCATTAACC
  TAGAGACCAGCTCATTACCTGTCGTGATGATTTCTAATGTCAGCCAACTACCTAATGCATGGGCATCCAT
  CATTTGGTACAATGTATCAACTAACGACTCCCAGAACTTGGTTTTCTTTAATAACCCTCCATCTGTCACT
  TTGGGCCAACTCCTGGAAGTGATGAGCTGGCAATTTTCATCCTATGTCGGTCGTGGCCTTAATTCAGAGC
  AGCTCAACATGCTGGCAGAGAAGCTCACAGTTCAGTCTAACTACAATGATGGTCACCTCACCTGGGCCAA
  GTTCTGCAAGGAACATTTGCCTGGCAAAACATTTACCTTCTGGACTTGGCTTGAAGCAATATTGGACCTA
  ATTAAAAAACATATTCTTCCCCTCTGGATTGATGGGTACATCATGGGATTTGTTAGTAAAGAGAAGGAAC
  GGCTTCTGCTCAAAGATAAAATGCCTGGGACATTTTTGTTAAGATTCAGTGAGAGCCATCTTGGAGGGAT
  AACCTTCACCTGGGTGGACCAATCTGAAAATGGAGAAGTGAGATTCCACTCTGTAGAACCCTACAACAAA
  GGGAGACTGTCGGCTCTGCCGTTCGCTGACATCCTGCGAGACTACAAGGTTATCATGGCTGAAAACATCC
  CTGAAAACCCTCTGAAGTACCTCTACCCTGACATTCCCAAAGACAAAGCCTTTGGCAAACACTACAGCTC
  CCAGCCGTGCGAAGTCTCAAGACCAACCGAACGGGGAGACAAGGGTTACGTCCCCTCTGTTTTTATCCCC
  ATTTCAACAATCCGAAGCGATTCCACGGAGCCACAATCTCCTTCAGACCTTCTCCCCATGTCTCCAAGTG
  CATATGCTGTGCTGAGAGAAAACCTGAGCCCAACGACAATTGAAACTGCAATGAATTCCCCATATTCTGC
  TGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011487
• Insert Size: 2247 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011487.5, NP_035617.1
• RefSeq Size: 3000 bp
• RefSeq ORF: 2247 bp
• Locus ID: 20849
• UniProt ID: P42228
• Cytogenetics: 1 26.67 cM
• Gene Summary: The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. Homozygous knockout mice for this gene exhibit reduced inflammation and cytokine production in response to immune challenge. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.