Adam10 (NM_007399) Mouse Untagged Clone

CAT#: MC221276

Adam10 (untagged) - Mouse a disintegrin and metallopeptidase domain 10 (Adam10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adam10
• Synonyms: 1700031C13Rik; kuz; kuzb; kuzbanian; MADM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NM_007399.3
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTTGCCGACAGTGTTAATTCTGCTCCTCTCCTGGGCGGCGGGGCTGGGAGGTCAGTATGGAAATC
  CTTTAAATAAATATATTAGACATTATGAAGGATTATCTTACAATGTGGATTCATTACACCAAAAACACCA
  GCGTGCCAAACGAGCAGTCTCACATGAGGACCAGTTTTTACTTCTAGATTTCCATGCTCATGGAAGACAG
  TTCAACCTACGAATGAAGAGGGACACTTCCCTTTTTAGTGATGAATTTAAAGTAGAAACATCAAATAAAG
  TACTTGATTATGATACCTCTCATATTTACACTGGACATATTTATGGTGAAGAAGGAAGCTTTAGTCATGG
  GTCTGTCATTGATGGAAGATTTGAAGGTTTCATCAAGACTCGTGGTGGCACGTTTTACATTGAGCCAGCA
  GAGAGATACATTAAAGATCGAATCCTGCCATTTCACTCTGTCATTTATCATGAAGATGATATTAACTATC
  CCCATAAATACGGCCCACAGGGGGGCTGTGCAGATCACTCCGTTTTTGAAAGGATGAGGAAGTACCAAAT
  GACTGGAGTAGAGGAAGGAGCCCGGGCACATCCAGAGAAGCATGCTGCTAGTAGTGGTCCTGAGCTCCTG
  AGGAAAAAACGCACAACTCTGGCTGAAAGAAATACTTGTCAGCTCTATATCCAGACAGATCACCTGTTCT
  TTAAATACTATGGAACACGAGAAGCTGTGATTGCTCAGATATCCAGTCATGTTAAAGCAATTGATACAAT
  TTACCAGACTACAGACTTCTCCGGAATCCGTAACATCAGCTTCATGGTGAAACGCATAAGAATCAATACA
  ACCTCTGATGAAAAAGACCCTACAAATCCTTTCCGTTTCCCAAATATTGGTGTGGAGAAGTTCCTGGAGT
  TGAATTCTGAGCAGAATCATGATGACTACTGCCTGGCCTATGTCTTCACAGACCGGGATTTTGATGATGG
  TGTTCTTGGTCTGGCCTGGGTTGGAGCACCTTCAGGAAGCTCTGGGGGAATATGTGAGAAAAGCAAGTTG
  TATTCAGATGGCAAGAAGAAGTCATTGAACACAGGCATCATTACTGTTCAGAACTATGGCTCCCATGTGC
  CTCCCAAAGTCTCTCATATTACGTTTGCTCATGAAGTTGGACATAACTTTGGATCTCCACATGATTCTGG
  AACAGAGTGTACTCCAGGAGAGTCTAAGAACTTAGGACAAAAAGAAAATGGCAATTACATCATGTATGCA
  AGAGCAACATCTGGGGACAAACTTAACAACAACAAATTTTCACTCTGCAGCATTAGAAACATAAGCCAAG
  TGCTTGAGAAGAAGAGGAACAACTGTTTTGTTGAATCTGGCCAGCCTATCTGTGGAAACGGGATGGTGGA
  ACAAGGGGAAGAGTGTGACTGTGGCTACAGTGACCAGTGCAAAGATGATTGCTGCTTCGATGCCAACCAG
  CCAGAGGGGAAGAAATGCAAGCTGAAGCCTGGGAAGCAGTGCAGTCCGAGTCAAGGACCCTGCTGTACAG
  CACAGTGTGCATTCAAGTCAAAGTCTGAAAAGTGCCGGGATGATTCTGACTGTGCAAAGGAAGGGATATG
  CAATGGCTTCACAGCCCTTTGCCCAGCATCTGATCCCAAGCCCAACTTTACAGACTGTAACAGGCACACA
  CAAGTGTGCATTAATGGGCAATGTGCAGGTTCTATTTGTGAAAAGTATGACTTGGAGGAGTGCACCTGTG
  CCAGCTCTGATGGCAAAGATGATAAGGAATTATGCCATGTTTGCTGCATGAAGAAAATGGCTCCATCAAC
  TTGTGCCAGTACAGGCTCTTTGCAGTGGAGCAAGCAGTTCAGTGGTCGGACTATCACTCTGCAGCCGGGC
  TCTCCATGTAATGACTTCAGAGGCTACTGTGATGTTTTCATGCGGTGCAGATTAGTAGATGCTGATGGCC
  CTCTAGCTAGGCTGAAAAAAGCCATTTTTAGTCCACAACTCTATGAAAACATTGCTGAGTGGATTGTGGC
  TCACTGGTGGGCAGTACTGCTTATGGGAATTGCCCTGATCATGTTAATGGCTGGATTTATCAAGATTTGC
  AGTGTTCACACTCCAAGTAGTAATCCAAAGTTGCCGCCTCCTAAACCACTTCCAGGCACTTTAAAGAGGA
  GGAGACCGCCACAGCCCATTCAGCAGCCCCCGCGTCAGAGGCCCCGAGAGAGTTATCAAATGGGACACAT
  GCGACGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007399
• Insert Size: 2250 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007399.3, NP_031425.2
• RefSeq Size: 4605 bp
• RefSeq ORF: 2250 bp
• Locus ID: 11487
• UniProt ID: O35598
• Cytogenetics: 9 39.53 cM
• Gene Summary: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature enzyme that is involved in the proteolytic release of membrane-bound proteins in a process called ectodomain shedding. Mice lacking the encoded protein die in utero with multiple defects of the developing central nervous system, somites, and cardiovascular system. [provided by RefSeq, May 2016]