Oas2 (NM_145227) Mouse Untagged Clone

CAT#: MC221262

Oas2 (untagged) - Mouse 2'-5' oligoadenylate synthetase 2 (Oas2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Oas2
• Synonyms: Oasl11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221262 representing NM_145227
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAACTGGCTGACTGGAAACTGGTCATCTGACAGGTCATCTGGCTATTCATCTGGCTGGTCACCTG
  GTGGGTCTTCAGGGGTGCCCTCCGGGCCAGTGCACAAGTTAGAAAAGTCTATCCAGGCAAACCTCACACC
  CAACGAAAACTGTCTGAAGCAGATTGCGGTGTCCTCGGTGCCATCGCAGAAGCTAGAAGGGTATATCCAG
  GAAAACCTCAAACCTAACAGAGAATCTCTGAAGCAGATAGACCAGGCCGTGGATGCCATCTGGGACCTGC
  TGCGCAGTCAGATCCCTGTGAAGGAAGTGGCTAAGGGTGGCTCCTATGGCCGGGAAACAGCCCTAAGAGG
  CTGCTCCGATGGTACCCTTGTTCTCTTCATGGACTGCTTCCAACAGTTCCAGGATCAGATAAAATACCAA
  GATGCATACCTTGACGTCATTGAACTGTGGCTGAAAATCCATGAGAAGAAGTCAGTAAAGCATGAACATG
  CCCTTGTAGTACAAGTGTCTGTACCAGGGCAGAGAATACTCCTGCAATTACTTCCAGTCTTCAATCCTCT
  ACGCTCCAATGAGAATCCCAGCTCCTGTGTCTATGTGGATCTCAAAAAATCCATGGATCAAGTAAGAGCC
  TCACCAGGGGAGTTCTCAGACTGCTTCACCACACTGCAGCAGCGGTTTTTCAAGAAATATCCCCAAAGAC
  TGAAGGATTTGATCCTATTGGTCAAGCACTGGTATGAACAGTGCCAGGAGAAGTGGAAAACACCCCCACC
  TCAGCCATTGCTGTACGCACTGGAACTGCTCACTGTGTATGCCTGGGAACAGGGCTGCCAAGCTGAAGAC
  TTCGACATGGCACAAGGCGTCAGGACCGTGCTGCGACTTATCCAGCGGCCGACAGAGCTGTGTGTCTACT
  GGACAGTCAATTACAACTTTGAGGATGAGACAGTCCGGAACATCCTTCTGCACCAGCTCAGGTCCCAAAG
  ACCAGTCATCTTGGATCCAACTGACCCAACCAATAATGTGGGCAAAGATGATGGGTTCTGGGAGCTACTG
  ACAGAGGAAGCTATGGCCTGGCTGTACTCTCCCAGCCTGAATACTGAGTCACCTGCACCATATTGGGATG
  TTCTGCCCATGCCACTTTTCGTCACTCCAAGCCACTTACTGAACAAGTTCATCAAGGACTTTCTCCAGCC
  CAACAAGCTCTTCCTAAAGCAGATCAAGGAAGCTGTTGACATTATATGTTCCTTCCTTAAAAATGTCTGC
  TTCTTGAATTCTGACACCAAAGTCCTGAAGACCGTCAAGGGAGGATCCACTGCCAAAGGCACAGCTCTGA
  AGCGGGGATCAGATGCTGACATTGTTGTGTTCCTCTCCTCGCTGGAGAGTTACGACTCTCTAAAAACCAA
  CCGCTCCCAGTTCGTCCAGGAGATCCAGAAGCAGTTAGAAGAATTCGTGCAGGCGCAGGAGTGGGAGGTG
  ACGTTTGAGATTTCAAAATGGAAGGCTCCCAGAGTGCTGAGTTTTACCTTGAAATCCAAGACTCTCAATG
  AAAGTGTCGAGTTCGATGTCCTTCCCGCCTATGATGCACTAGGTCAACTGCGGTCTGACTTCACCCTCAG
  GCCCGAAGCCTACAAGGATCTCATTGAGCTGTGTGCATCACAGGACATCAAAGAAGGAGAGTTTTCTATC
  TGTTTTACTGAGCTGCAGAGAAACTTCATTCAAACCCGGCCCACCAAACTGAAGAGTCTACTCCGCCTGA
  TCAAGCACTGGTACAAACAGTATGAAAGGAAGATGAAGCCAAAAGCATCTTTACCCCCAAAGTACGCCCT
  GGAGCTGCTCACCGTGTATGCCTGGGAGCAGGGCAGTGGCACAGATGACTTTGACATTGCTGAAGGCTTC
  CGGACCGTCCTGGACCTGGTTATAAAATACCGGCAGCTCTGCATCTTCTGGACAGTCAATTACAACTTTG
  AAGAGGAATACATGCGGAAGTTCCTACTGACCCAGATCCAGAAAAAGAGGCCTGTAATCCTGGATCCAGC
  AGATCCCACAGGCGATGTGGGAGGAGGTGACCGCTGGTGCTGGCATCTTCTAGCTGAAGAAGCGAAGGAG
  TGGCTGTCCTCCCCTTGTTTCCAAGTGGAGCAAAAAGGCCTGGTACAGCCTTGGAAAGTGCCAGTAATGC
  AGACCCCCGGAAGCTGTGGAGGTCAGATCTACCCCACTGTGGGTGGAGTTACTAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145227
• Insert Size: 2229 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145227.3, NP_660262.2
• RefSeq Size: 3873 bp
• RefSeq ORF: 2229 bp
• Locus ID: 246728
• UniProt ID: E9Q9A9
• Cytogenetics: 5 60.64 cM
• Gene Summary: Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response (PubMed:12396720, PubMed:29117179). Activated by detection of double stranded RNA (dsRNA): polymerizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNASEL) leading to its dimerization and subsequent activation (PubMed:29117179). Activation of RNASEL leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication (PubMed:21142819). Can mediate the antiviral effect via the classical RNASEL-dependent pathway or an alternative antiviral pathway independent of RNASEL (PubMed:21142819). In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation (PubMed:21142819). May act as a negative regulator of lactation, stopping lactation in virally infected mammary gland lobules, thereby preventing transmission of viruses to neonates (PubMed:29117179). Non-infected lobules would not be affected, allowing efficient pup feeding during infection (PubMed:29117179).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate coding exon in the 3' end compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.