Carmil2 (NM_001033320) Mouse Untagged Clone

CAT#: MC221258

Rltpr (untagged) - Mouse RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing (Rltpr), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Carmil2
• Synonyms: CG1399-PB; D130029J02Rik; Gm585; Rltpr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221258 representing NM_001033320
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCTGCCACTGAATGACGTAACCCAGGCTCATCGCAGCCGGCCAGAACTCACAACTCGAGCGGTCC
  ATCAGATCCAAGCCTGTCTCTGGAGGAACAACCAAGTAGACTCTACTTCGGACCTCAAGCCCTGCCTTCA
  GCCCTTAGGTCTGATTTCAGACCACTCAGAGCAGGAGGTGAACGAGCTATGCCAGTCAGTACAGGAGCAT
  ATGGAGCTGCTGGGCTGTGGGGCTGGGCCCCAGGGTGAGGTTGCCGTGCACCAGGCTGAGGACGCCATCC
  AGAATGCCAACTTCTCTCTCAGTATCCTCCCCATTCTCTATGAGGCTGGGAGATCCCCAAGCCACCACTG
  GCAGCTGCAGCAGAAGCTAGAGAGCCTCCTGGGTCAGGTGGGCGAGATCTGCCGCCAGGACATCCAGGAC
  TTCACTCAGACCACCCTGGATACCACAAGGAGCCTCTGCCCACAGATGTTGCAGACACCTGGCTGGAGGA
  AGCAGCTAGAGGGAGTTCTGGTGGGCTCCGGGGGCCTCCCAGAGCTGCTTCCGGAACATCTGCTGCAAGA
  TGCCTTCTCTAGGCTGAGGGACATGCGCCTGTCAATCACTGGGACCCTAGCAGAGAGCATTGTGGCTCAG
  GCTCTTGCAGGTCTTCATGCAGCCCGAGATCGACTGGTGGAGAGGCTAACTCAGCAGGCACCAGTGACCA
  TGGCCCCTGCTGTACCACCACTGGGTGGAAATGAGCTCAGCCCCCTTGAGACTGGGGGATTGGAAGAGCT
  TTTCTTTCCCACGGAGAAGGAAGAGGAGAGAGAAAAGGATGAGAGTTCTTCATGGAAATGGCTTGAGCCT
  AGTAACTGTTTTCACCTGGTCTCCTCCCTTCATGGTGCTGCTGAGGAAGCGGAACGGGACCCCGAGCTGG
  CAGCTCCGGGGGAAGATGCAGAGCCGCAGGCTGGGCCGTCTGCACGCGGCTCTCCAAGCCCCGCCGCCCC
  GGGGCCACCCGCCGGCCCTCTGCCTCGCATGGACCTGCCACCCGCCGGGCAACCCCTACGCCACCCAACC
  CGAGCCCGACCGAGACCACGTCGCCAGCACCACCACCGCCCGCCGCCGGGGGGCCCCCAGGTGCCCCCAG
  CCCTGCTTCAAGAAGGAAATGGGCTCACTGCTCGCGTGGATGAGGGTGTGGAGGAGTTCTTCTCCAAAAG
  GCTGATCCAGCAGGATCACTTCTGGGCCCCAGAGGAGGATCCAGCCACTGAGGGTGGTGCCACTCCTGTC
  CCCCGCACACTTCGAAAGAAGCTGGGTACGCTCTTTGCCTTTAAGAAACCTCGTTCAACAAGGGGTCCGC
  GACCTGACCTGGAGACCAGCCCTGGAGCAGCAGCTCGAGCCAGAAAATCCACACTTGGGGATCTCCTGCG
  ACCACCGGCCCGTCCAGGCCGTGGTGAGGAACCTGGAGGGGCGGAAGGCGGCACCAGCAGCCCTGACCCT
  GCTCGCAGAAATCGGCCTCGGTACACGCGGGAAAGCAAGGCCTACTCCATGATCCTGCTACCTGCTGAGG
  AGGAAGCAGCCGTGGGTACCAGACCTGATAAGAGGCGGCCTCTGGAACGGGGAGACACAGAACTGGCTCC
  ATCTTTTGAGCAGCGGGTACAAGTGATGCTACAGAGGATCGGCGTGAGCCGGGCCAGTGGGGGTGCCGAG
  AGCAAGAGGAAGCAAAGTAAAGACGGCGAGATCAAGAAGGCAGGCTCTGATGGTGACATTATGGACAGTT
  CCACAGAGACCCCTCCCATCTCAATCAAGTCCCGTACCCACTCTGTGTCTGCTGATCCCTCATGCAGACC
  TGGGCCAGGAGGCCAGGGGCCTGAGTCTGCCACCTGGAAGACATTGGGACAGCAGCTGAATGCAGAGCTC
  AGAGGCCGTGGTTGGGGCCAACAGGATGGTCCAGGGCCCCCCTCCCCATGTCCCAGCCCAAGTCCCCGAA
  GAACCAGCCCCGCCCCAGACATCCTGAGTCTCCCAGAGGACCCCTGCTTGGGCCCTAGGAATGAAGAACG
  GCCCCTCCGGCTGCAGCGCTCCCCTGTCCTCAAGCGTAGGCCGAAGCTCGAGGCACCCCCATCCCCAAGC
  TTAGGCTCTGGCCTTGGATCCAAGCCTCTTCCTCCGTACCCCACAGAACCCTCCAGCCCTGAGCGGAGCC
  CTCCCTCCCCAGCCACAGACCAAAGAGGCGGCGGCCCCAACCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033320
• Insert Size: 2217 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033320.2, NP_001028492.1
• RefSeq Size: 2504 bp
• RefSeq ORF: 2217 bp
• Locus ID: 234695
• UniProt ID: Q3V3V9
• Cytogenetics: 8 D3
• Gene Summary: Cell membrane-cytoskeleton-associated protein that plays a role in the regulation of actin polymerization at the barbed end of actin filaments. Prevents F-actin heterodimeric capping protein (CP) activity at the leading edges of migrating cells, and hence generates uncapped barbed ends and enhances actin polymerization. Plays a role in cell protrusion formations; involved in cell polarity, lamellipodial assembly, membrane ruffling and macropinosome formations. Involved as well in cell migration and invadopodia formation during wound healing (By similarity). Required for CD28-mediated stimulation of NF-kappa-B signaling, involved in naive T cells activation, maturation into T memory cells, and differentiation into T helper cells (PubMed:27647348). Required for CD28-mediated differentiation of T regulatory cells (By similarity).[UniProtKB/Swiss-Prot Function]