Papolg (NM_172555) Mouse Untagged Clone

CAT#: MC221255

Papolg (untagged) - Mouse poly(A) polymerase gamma (Papolg), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Papolg
• Synonyms: 9630006B20Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221255 representing NM_172555
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGAGATGTCTGCAAACACTATGCTGGACAGCCAGCGTCAACAGAAGCATTATGGAATTACCTCTC
  CAATTAGTTTGGCATGTCCTAAAGAAATTGATCATATTTATACACAGAAGTTAATTGATGCCATGAAGCC
  ATTTGGAGTATTTGAAGATGAGGAAGAATTGAACCACAGGCTGGTTGTTCTTGGTAAATTGAACAATTTA
  GTAAAAGAATGGATTTCTGACATCAGTGAAAGTAAGAATCTCCCACCTTCTGTTGTGGCTACTGTTGGTG
  GTAAAATTTTCACATTTGGATCTTACAGGCTTGGAGTACACACCAAAGGAGCTGATATTGATGCACTTTG
  TGTTGCCCCAAGACATGTAGAGAGATCAGATTTTTTTCAGTCCTTTTTTGAAAAATTGAAACATCAAGAT
  GGCATTAGAAACTTAAGAGCTGTAGAAGATGCTTTTGTACCTGTTATAAAATTTGAATTTGATGGTATTG
  AAATTGATCTAGTCTTTGCAAGACTGGCAATACAAACCATATCAGATAATTTAGACCTAAGAGATGACTC
  TCGCCTGAGAAGCCTTGATATACGGTGCATTAGAAGTTTAAATGGATGTAGAGTTACTGATGAAATTTTG
  CACTTAGTGCCAAATAAAGAAACTTTTAGACTCACCCTAAGAGCAGTCAAATTATGGGCAAAGCGCCGTG
  GTATTTATTCCAATATGCTGGGATTCCTTGGTGGTGTCTCCTGGGCAATGCTAGTTGCCAGAACTTGCCA
  ACTGTACCCAAATGCAGCAGCTTCAACTTTAGTTCACAAGTTTTTTTTAGTTTTTTCCAAATGGGAATGG
  CCAAATCCTGTGCTGTTGAAGCAACCAGAAGAAAGCAACTTGAACTTGCCTGTCTGGGATCCTCGGGTAA
  ATCCGTCAGACAGGTATCATCTTATGCCAATAATTACCCCTGCCTACCCACAACAGAATTCTACATATAA
  TGTGTCTACATCAACTCGAACAGTAATGGTAGAAGAATTTAAACAAGGTCTTGCAGTTACAGATGAAATT
  CTTCAAGGGAAATCAGATTGGTCCAAACTATTAGAGCCACCAAATTTCTTTCAAAAATACAGACATTATA
  TAGTACTGACTGCCAGTGCATCAACAGAAGAGAACCATCTAGAATGGGTTGGATTAGTAGAATCTAAAAT
  CCGTGTACTTGTTGGAAACTTGGAACGGAATGAATTTATTACTCTTGCCCATGTGAATCCCCAGTCATTC
  CCAGGAAATAAAGAACATCATAAAGCCAACAATTACGTATCAATGTGGTTCCTTGGGATAATTTTTCGGA
  GAGTAGAAAATGCAGAAAGTGTTAACATAGACTTGACATATGATATCCAGTCATTTACTGATACAGTGTA
  CAGGCAGGCAAACAATATAAATATGCTGAAGGATGGGATGAAAATTGAAGCAACTCATGTAAAGAAAAAG
  CAGCTTCATCATTACCTACCAGCAGAGATTCTTCAAAAGAAGAAAAAGAGTCTTTCTGATGTAAGTCGGA
  GTTCAGGGGGCCTTCAGTCCAAAAGATCATCTCTGGATAGCACTTGTCTGGATAGCTCCAGAGACACTGA
  CAGTGGAACACCTTTTAACTCTCCGGTGTCTGCAAATAAGCCTTCCAACCCAGACAGCCCCACAGGAGAA
  ATAGAAAGGAGTAGTGCTGAGCCTGTGGCTGTGGTTGTGGAGAAGCTGCCAAGTGTCCCCCCAGCTCAGG
  GGCTGTCCATTCCAGTCATTGGTGCAAAGGTTGACCCTACAGCAAAAGCTGTATCCTCCCCAGCTGTGTG
  CACCATTCCTACTGTAGTAGGACGGAATGTTATTCCTAGAATCACAACACCCCACAACCCTGTCCAGGGA
  CAACCACATCTAAATGGGATATCAAATATTACTAAGAATGTTACACCCAAAAGATCCCATTCGCCACCTA
  CAGATGGGACTTCTAAGAGGCTGAAAGACATAGAAAAGTTCATTCGACTTGAGTCAGCATTTAAGGAGTC
  CCGGGCTGCCGAGGATCGGAAAAGAAAACCAATGGATTCGATTGGTGGAGAATCTATGCCTATTCCAACT
  ATTGATACGGCACGCAAAAAGAGACTACCCAGTAAAGAACTCCCAGATTCATCATCTCCAGTTCCAGCAA
  ACAACATCCGTGTCATCAAAAACTCGATCCGCCTGACCCTTAATCGGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172555
• Insert Size: 2220 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172555.2, NP_766143.2
• RefSeq Size: 3776 bp
• RefSeq ORF: 2220 bp
• Locus ID: 216578
• UniProt ID: Q6PCL9
• Cytogenetics: 11 A3.2
• Gene Summary: Responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.[UniProtKB/Swiss-Prot Function]