Asph (NM_023066) Mouse Untagged Clone

CAT#: MC221237

Asph (untagged) - Mouse aspartate-beta-hydroxylase (Asph), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Asph
• Synonyms: 2310005F16Rik; 3110001L23Rik; AI848629; AW261690; AW561901; BAH; C79816; cI-37
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221237 representing NM_023066
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCGCGCAAGAACGCCAAGGGCGGCGGCGGCAACAGCAGCAGCAGCGGCAGCGGCAGCGGCTCGG
  GCAGCGGTAGCCCGAGCACGGGTAGCAGCGGTAGCAGCAGTAGCCCCGGGGCTCGGAGAGAGGCAAAGCA
  CGGAGGACACAAGAATGGGAGAAGAGGAGGGATTTCAGGAGGGTCCTTTTTCACATGGTTCATGGTCATT
  GCATTGCTCGGCGTCTGGACATCTGTGGCTGTCGTGTGGTTTGACTTGGTCGATTATGAAGAAGTTCTAG
  GAAAACTAGGAGTCTATGATGCGGATGGCGATGGAGACTTTGATGTGGATGATGCCAAAGTTTTATTAGG
  CCTTAAAGAAAGATCTCCTTCTGAGAGGACATTTCCACCGGAGGAGGAGGCAGAGACTCACGCTGAGCTG
  GAGGAGCAGGCCCCTGAGGGGGCAGACATCCAGAATGTTGAAGATGAAGTAAAGGAACAAATTCAGTCCC
  TTCTTCAGGAATCAGTACACACAGACCATGACCTTGAAGCAGATGGGCTGGCAGGAGAACCACAGCCGGA
  GGTTGAGGACTTCCTCACAGTGACCGACAGTGATGACAGATTTGAGGACCTGGAACCCGGGACAGTTCAT
  GAAGAAATCGAGGATACTTACCATGTGGAAGACACAGCATCGCAGAACCATCCAAATGACATGGAAGAGA
  TGACGAATGAGCAGGAAAATTCAGATCCCAGTGAAGCAGTGACAGATGCAGGTGTGCTGCTGCCCCATGC
  AGAGGAAGTAAGACACCAAGACTATGATGAACCAGTATATGAACCCTCAGAGCATGAAGGGGTCGCGATT
  TCAGATAACACCATAGATGATTCCAGCATAATCTCAGAAGAAATAAATGTCGCCTCTGTTGAAGAACAGC
  AAGACACACCACCAGTTAAGAAGAAGAAGCCTAAACTTCTGAACAAATTTGATAAGACAATTAAGGCTGA
  GCTGGATGCTGCAGAAAAGCTCCGGAAAAGGGGTAAAATTGAGGAAGCAGTGAACGCATTTGAAGAACTG
  GTTCGAAAGTACCCTCAGAGCCCACGAGCAAGATATGGCAAAGCGCAGTGTGAAGATGACTTGGCAGAGA
  AGCAGAGAAGCAATGAGGTTCTGCGCAGGGCCATCGAGACCTACCAGGAGGCAGCCGACCTGCCTGATGC
  CCCTACAGACCTGGTGAAGCTGAGCTTGAAGCGAAGGTCGGAACGGCAGCAGTTTCTAGGTCACATGAGA
  GGTTCTCTACTTACCCTCCAGAGACTAGTTCAACTGTTCCCTAGTGATACTACCTTAAAAAACGACCTTG
  GCGTAGGATACCTCTTGTTGGGAGACAACGACAGTGCCAAGAAGGTTTACGAAGAGGTGCTAAATGTGAC
  ACCAAATGATGGCTTCGCTAAAGTGCATTACGGCTTCATCCTGAAGGCACAGAACAAGATATCTGAGAGC
  ATTCCCTACTTAAAGGAAGGAATCGAATCTGGGGACCCTGGCACGGATGATGGCCGGTTTTACTTCCACT
  TGGGGGATGCCATGCAGAGGGTCGGGAACAAAGAGGCATATAAGTGGTATGAACTTGGGCACAAGAGAGG
  ACATTTTGCCTCTGTCTGGCAGCGTTCCCTCTACAATGTGAATGGTCTGAAGGCTCAGCCGTGGTGGACA
  CCCAGGGAGACTGGCTACACAGAGCTAGTGAAGTCTTTAGAGAGAAACTGGAAGTTAATCCGTGATGAAG
  GCCTCATGGTGATGGATAAAGCCAAGGGTCTCTTCCTGCCTGAGGACGAAAACCTTCGGGAGAAGGGCGA
  CTGGAGCCAGTTCACACTGTGGCAGCAAGGAAGGAAGAATGAGAATGCCTGTAAAGGAGCGCCTAAGACC
  TGTGCTTTACTAGAAAAGTTTTCCGAAACAACAGGATGCAGAAGAGGACAGATCAAATACTCCATCATGC
  ACCCTGGAACTCATGTGTGGCCGCATACAGGACCCACAAACTGCAGGCTCCGAATGCATCTGGGGTTAGT
  GATCCCCAAGGAAGGCTGCAAGATCCGGTGTGCCAATGAGACCAGGACGTGGGAAGAAGGCAAGGTGCTC
  ATCTTTGATGACTCTTTTGAGCACGAGGTTTGGCAGGATGCCTCGTCTTTCCGGCTGATATTCATCGTGG
  ATGTGTGGCACCCCGAGCTGACCCCTCAGCAGAGACGCAGCCTTCCCGCAATTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023066
• Insert Size: 2226 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023066.3, NP_075553.2
• RefSeq Size: 6688 bp
• RefSeq ORF: 2226 bp
• Locus ID: 65973
• UniProt ID: Q8BSY0
• Cytogenetics: 4 A1
• Gene Summary: Isoform 1: specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.