P3h1 (NM_019783) Mouse Untagged Clone

CAT#: MC221234

Lepre1 (untagged) - Mouse leprecan 1 (Lepre1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: P3h1
• Synonyms: 2410024C15Rik; Gros1; Lepre1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221234 representing NM_019783
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGAGCGAAAGGAGGCTGCTGGCAGCGATGCTAGCGGTCGCCGCCGCCGCCGCCTTACGGGTTG
  CGGCGGAGTCTGAGCCGGGATGGGACGTGGCAGCCCCTGACCTGCTTTACGCAGAGGGGACCGCGGCCTA
  CTCGCGCGGGGACTGGCCCGGGGTGGTCCTGAACATGGAGCGGGCGCTGCGCTCGCGGGCGGCCCTGCGT
  GCCCTCCGCCTGCGCTGCCGCACACGCTGTGCCACCGAACTGCCGTGGGCACCGGACCTGGATCTCGGTC
  CGGACCCCAGCCTGAGCCAGGACCCGGGCGCCGCCGCCCTGCACGACCTGCGCTTCTTCGGAGCCGTGCT
  GCGCCGTGCCGCCTGCCTACGCCGCTGCCTCGGGCCGCCCTCTGCCCACTTGCTGAGTGAAGAACTGGAC
  CTGGAGTTCAACAAGCGGAGCCCGTACAACTACCTGCAGGTCGCCTATTTCAAGATAAACAAGCTGGAGA
  AAGCTGTGGCTGCGGCACACACCTTCTTTGTGGGCAATCCTGAGCACATGGAGATGCGGCAGAACCTCGA
  CTATTACCAAACCATGTCTGGGGTGAAGGAGGCAGACTTCAGGGATCTCGAGGCCAAGCCCCATATGCAT
  GAGTTTCGGCTGGGGGTACGACTCTACTCAGAGGAGAAGCCACAGGAAGCTGTGCCCCACCTGGAGGCGG
  CACTGCAAGAGTACTTTGTGGCCGATGAGGAATGCCGTGCCCTCTGCGAAGGGCCCTATGACTACGACGG
  CTACAACTACCTAGACTACAGCGCTGACCTCTTCCAGGCCATCACAGATCATTACGTCCAGGTCCTCAAC
  TGTAAGCAGAACTGTGTCACGGAGCTGGCTTCCCACCCAAGTAGGGAAAAGCCCTTTGAAGACTTCCTCC
  CTTCACACTATAATTACCTACAGTTTGCCTACTACAACATTGGGAACTATACACAAGCTATTGAATGTGC
  CAAGACCTACCTCCTCTTCTTTCCCAATGATGAGGTGATGCACCAGAATCTGGCTTATTACACAGCCATG
  CTTGGAGAAGAAGAGGCCAGCTCCATCAGCCCCAGGGAGAATGCCGAGGAATACCGACGTCGAAGCCTGT
  TGGAGAAAGAACTGCTTTTCTTCGCTTATGACATTTTTGGAATTCCCTTTGTGGATCCGGACTCATGGAC
  TCCAGAAGAGGTGATTCCCAAGAGACTGCAGGAGAAACAGAAGTCGGAACGCGAAACCGCAGTCCGCATC
  TCCCAGGAGATTGGCAACCTTATGAAGGAGATCGAGACCCTCGTGGAGGAGAAGACCAAGGAGTCCTTGG
  ATGTGAGCCGACTGACGCGGGAAGGTGGTCCCCTGCTGTATGAAGGCATCAGTCTCACCATGAATTCCAA
  AGTCTTGAATGGCTCCCAGCGGGTGGTGATGGATGGTGTGATCTCTGATGATGAGTGCCAGGAGCTGCAG
  AGACTGACCAATGCGGCAGCAACTTCGGGAGATGGCTACCGAGGTCAGACCTCCCCACACACCCCAAATG
  AAAAGTTCTATGGTGTTACTGTCCTCAAAGCTCTCAAGCTCGGGCAGGAAGGAAAAGTTCCTCTGCAGAG
  TGCCCGCATGTACTACAACGTGACAGAGAAGGTGCGGCGCGTCATGGAGTCCTACTTCCGCCTGGACACG
  CCCCTCTATTTCTCTTATTCCCACCTCGTGTGCCGCACTGCAATAGAAGAGTCACAGGCTGAGAGGAAGG
  ACAGTAGCCACCCCGTCCACGTGGATAACTGCATCCTGAATGCCGAAGCCCTCATGTGTATCAAGGAGCC
  CCCAGCCTACACGTTCCGGGACTACAGCGCCATCCTTTACCTCAATGGCGACTTCGATGGAGGAAACTTT
  TACTTCACAGAACTAGATGCCAAGACTGTGACGGCAGAGGTGCAGCCCCAGTGTGGAAGGGCTGTGGGAT
  TCTCTTCTGGCACTGAGAACCCACATGGAGTGAAGGCTGTCACCAGGGGGCAGCGCTGCGCCATCGCCCT
  GTGGTTCACGCTGGATCCTCGGCACAGTGAGAGAGACAGGGTGCAGGCAGATGACCTGGTGAAGATGCTG
  TTCAGCCCAGAAGAGGTGGACCTCCCCCAGGAACAGCCCCTGCCTGACCAGCAGGGCTCGCCAGAGCCTG
  GAGAAGAGTCTCTGTCAGACAGAGGGTCGCTGCACAAGGATGAGCTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019783
• Insert Size: 2220 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019783.2, NP_062757.2
• RefSeq Size: 3210 bp
• RefSeq ORF: 2220 bp
• Locus ID: 56401
• UniProt ID: Q3V1T4
• Cytogenetics: 4 D2.1
• Gene Summary: Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses alternate splice sites in the 3' coding region and differs in the 3' UTR, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct C-terminus, compared to isoform 1.