Mefv (NM_001161791) Mouse Untagged Clone

CAT#: MC221233

Mefv (untagged) - Mouse Mediterranean fever (Mefv), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mefv
• Synonyms: FMF; pyrin; TRIM20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221233 representing NM_001161791
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGACCCTTGGTGATCATCTGCTAAACACCCTGGAGGAGCTGCTGCCCTATGACTTTGAGAAGT
  TCAAGTTTAAGCTACAGAACACCAGCCTGGAGAAGGGCCACTCTAAGATCCCCCGGGGCCATATGCAGAT
  GGCCAGGCCAGTAAAGCTGGCCAGCCTTCTGATAACCTACTACGGGGAGGAGTATGCTGTAAGGCTGACC
  CTGCAGATCCTGCGGGCCACCAACCAGCGCCAACTAGCAGAGGAGCTTCGCAAAGCCACAGGCACAGAAC
  ATTTAATTGAAGAAAATAGAGTTGGTGGTTCTGTCCAGTCTTCTGTAGAGAATAAAGCCAAGAGTGTGAA
  GGTACCAGATGTCCCTGAAGGTGATGGGACACAGCAAAACAATGATGAATCAGACACCCTTCCATCCAGC
  CAGGCTGAAGTGGGGAAGGGACCCCAGAAGAAGTCACTGACCAAAAGGAAGGATCAGAGGGGCCCCGAGA
  GCCTGGACTCACAGACCAAGCCATGGACAAGGAGCACAGCGCCTCTCTATAGGAGAACCCAGGGCACCCA
  GTCCCCAGGGGATAAGGAGAGCACAGCAAGTGCCCAGCTCCGCAGGAATGTCAGCTCTGCAGGGAGGCTT
  CAAGGACTTTACAACAATGCCCCAGGGAGGAGAGAAAGCAAGAAAGCCGAAGTGTATGTATATTTGCCTT
  CAGGAAAGAAGCGACCCAGGAGTCTTGAAATTACCACTTATTCAAGAGAAGGAGAACCCCCAAATTCAGA
  AGTTCTTCCAACTCAAGAGGAAACAAGAAATGGGAGTCTCATTCGCATGAGAACAGCTACTCTGAATGGA
  AGGACTACGGGGGCTTTAGAAAAGGGCACAGGGATTCCAGAGCATTCCATGGTGCTGGATGAAAAAACAT
  TCAGAAACATGTCTTCCAAAACATCGTTGATTGGGGAGGAGAGATGCCCTACATCATGGACGGAAAATGG
  AAATGGGAGTCCAGAAACCACAGAGTCCTCGGGAGAGACGGCTGGCAGCATACTCTCTGATCCAGAAGTG
  CCTTTGTCACTATGTGAAAAACCAGCTAAAACTCCAGAAGACCCAGCATCCTTAGGACAGGCCGCTTGTG
  AAGGAAGGTCACAGGACAAGGCTGTGTGCCCTCTTTGCCACACCCAGGAAGGAGACCTGCGTGGTGATAC
  CTGTGTGCAAAGTTCCTGTAGCTGCTCCATTGCTCCTGGGGATCCCAAGGCCTCCGGCAGATGCTCCATA
  TGCTTTCAGTGCCAAGGCTTACTTGCCAGAAAGAGCTGCGAAGCCCAGAGCCCCCAGTCCCTACCACAGT
  GCCCTCGTCACATGAAGCAGGTGCTGCTGCTTTTCTGTGAGGACCACAGGGAGCCCATCTGCCTCATCTG
  CAGGCTGAGCCTGGAGCATCAAGGACATCGAGTGCGCCCCATAGAGGAGGCTGCACTAGAGTACAAGGAG
  CAGATCCGGGAGCAGCTGGAGCGCCTGCGGGAGATGAGGGGATATGTGGAGGAGCACAGGCTACAGGGAG
  ACAAGAAAACAGACGATTTCCTGAAACAAACAGAAATCCAGAAGCAGAAGATTTCATGTCCACTTGAGAA
  GCTATACCAACTCCTGGAGAAGCAAGAACAACTCTTTGTGACCTGGCTACAGGAGCTAAGCCAGACCATC
  AGCAAGGTCAGAGAGACATACTACACCCGGGTTTCCCTACTGGATGAGATGATTGAAGAACTAGAGGCCA
  AGCAAGACCAGCCAGAGTGGGACCTCATGCAGGACATTGGAATCACCTTGCACAGGGCTAAGATGATGTC
  TGCCTCTGAGCTGTTGGACACTCCTCCAGGTGTTAAAGAAAAGCTTCATCTGCTCTACCAGAAGTCAAAG
  TCTGTGGAGAAGAATATGCAGTGTTTCTCAGAAATGCTGAGCTCTGAAATGGCATTCAGCGCTTCAGATG
  TAGCAAAATGGGAAGGGCGCCAGCCTAGCGCCACACAGGTCCAGGGCTTGGTTCCCACCGTTCACCTAAA
  GTGTGATGGTGCACACACACAGGACTGTGATGTAGTATTTTACCCAGAACGGGAAGCTGGAGGATCAGAA
  CCTAAAGATTACCTTCATCCTCAGCCAGCTCAAGACACACCTGAGCTACATGAGATCCATTCTCGGAACA
  ACAAAAGAAAATTTAAATCTTTCCTGAAATGGAAACCTTCATTCTTACCGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001161791
• Insert Size: 2223 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161791.1, NP_001155263.1
• RefSeq Size: 3094 bp
• RefSeq ORF: 2223 bp
• Locus ID: 54483
• Cytogenetics: 16 2.18 cM
• Gene Summary: Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence preventing excessive IL1B- and IL18-mediated inflammation. However, it may also have a positive effect in the inflammatory pathway. In different experimental systems, it has been shown to activate IL1B production. It has also been shown to be required for PSTPIP1-induced PYCARD oligomerization and for formation of inflammasomes. Recruits PSTPIP1 to inflammasomes, and is required for PSTPIP1 oligomerization.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' UTR and has multiple coding region differences compared to variant 1, one of which results in a frameshift. This results in a shorter isoform (2) with a distinct C-terminus, compared to isoform 1.