Prkce (NM_011104) Mouse Untagged Clone

CAT#: MC221225

Prkce (untagged) - Mouse protein kinase C, epsilon (Prkce), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prkce
• Synonyms: 5830406C15Rik; Pkce; PKCepsilon; PKC[e]; R75156
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221225 representing NM_011104
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGTGTTCAATGGCCTTCTTAAGATCAAAATCTGCGAGGCGGTGAGCTTGAAGCCCACAGCCTGGT
  CGCTGCGCCATGCGGTGGGACCCCGGCCACAGACGTTCCTTTTGGACCCCTACATTGCCCTTAACGTGGA
  CGACTCGCGCATCGGCCAAACAGCCACCAAGCAAAAGACCAACAGCCCGGCCTGGCACGATGAGTTCGTC
  ACCGATGTGTGCAATGGGCGCAAGATCGAGCTGGCTGTCTTTCACGACGCTCCTATCGGCTACGACGACT
  TCGTGGCCAACTGCACCATCCAGTTCGAGGAGCTGCTGCAGAATGGGAGCCGTCACTTCGAGGACTGGAT
  TGACCTGGAGCCAGAAGGAAAAGTGTACGTGATCATCGATCTCTCGGGATCATCGGGTGAAGCCCCTAAA
  GACAATGAAGAACGAGTGTTCAGGGAGCGTATGCGGCCAAGGAAGCGGCAAGGGGCTGTCAGGCGCAGGG
  TCCACCAGGTCAATGGCCACAAGTTCATGGCCACCTACTTGCGGCAACCCACCTACTGCTCCCACTGCAG
  AGATTTCATCTGGGGTGTCATAGGAAAACAGGGATATCAATGTCAAGTTTGCACTTGCGTTGTCCACAAG
  CGATGTCATGAGCTCATTATTACAAAGTGCGCTGGGCTGAAGAAACAGGAAACCCCTGACGAGGTGGGCT
  CCCAACGGTTCAGCGTCAACATGCCCCACAAGTTCGGGATCCACAACTACAAGGTCCCCACGTTCTGTGA
  CCACTGTGGGTCCCTGCTCTGGGGCCTCTTGCGGCAGGGCTTGCAGTGTAAAGTCTGCAAAATGAATGTT
  CACCGGCGATGTGAGACCAATGTGGCTCCCAACTGTGGGGTAGACGCCAGAGGAATTGCCAAAGTGCTGG
  CTGACCTTGGTGTTACTCCAGACAAAATCACCAACAGTGGCCAAAGGAGGAAAAAGCTCGCTGCTGGTGC
  TGAGTCCCCACAGCCGGCTTCTGGAAACTCCCCATCTGAAGACGACCGATCCAAGTCAGCGCCCACCTCC
  CCTTGTGACCAGGAACTAAAAGAACTTGAAAACAACATTCGGAAGGCCTTGTCATTTGACAACCGAGGAG
  AGGAGCACCGAGCGTCGTCGGCCACCGATGGCCAGCTGGCAAGCCCCGGAGAGAACGGGGAAGTCCGGCC
  AGGCCAGGCCAAGCGCTTGGGGCTGGATGAGTTCAACTTCATCAAGGTGTTGGGCAAAGGCAGCTTTGGC
  AAGGTCATGTTGGCGGAACTCAAAGGCAAAGATGAAGTCTACGCTGTGAAGGTCTTGAAGAAGGACGTTA
  TCCTACAAGACGATGATGTGGACTGCACAATGACAGAGAAGAGGATTTTGGCTCTGGCTCGGAAACACCC
  TTATCTAACCCAACTCTATTGCTGCTTCCAGACCAAGGACCGCCTCTTCTTCGTCATGGAATATGTAAAT
  GGTGGAGACCTCATGTTCCAGATTCAGCGGTCCCGAAAATTTGATGAGCCTCGTTCTCGGTTCTATGCCG
  CAGAGGTCACATCAGCCCTCATGTTTCTCCACCAGCACGGAGTGATCTACAGGGATTTGAAACTGGACAA
  CATCCTTCTAGATGCAGAAGGCCACTGCAAGCTGGCTGACTTTGGGATGTGCAAGGAAGGGATTATGAAT
  GGTGTGACAACTACCACCTTCTGTGGGACTCCTGACTACATAGCTCCAGAGATCCTACAGGAGTTGGAGT
  ACGGCCCCTCAGTGGACTGGTGGGCCCTGGGGGTGCTGATGTACGAGATGATGGCTGGGCAGCCCCCCTT
  TGAAGCTGACAACGAGGACGACTTGTTCGAATCCATCCTTCATGATGATGTTCTCTATCCTGTCTGGCTT
  AGCAAGGAAGCTGTCAGCATCCTGAAAGCTTTCATGACCAAGAACCCGCACAAGCGCCTGGGCTGTGTGG
  CAGCGCAGAACGGGGAGGACGCCATCAAGCAACATCCATTCTTCAAGGAGATTGACTGGGTACTGCTGGA
  GCAGAAGAAAATCAAGCCCCCCTTCAAGCCGAGAATTAAAACCAAAAGAGATGTCAATAACTTTGACCAA
  GACTTTACGCGGGAAGAGCCAATACTTACACTTGTGGATGAAGCAATCATTAAGCAGATCAACCAGGAAG
  AATTCAAAGGCTTCTCCTACTTTGGTGAAGACCTGATGCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011104
• Insert Size: 2214 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011104.3, NP_035234.1
• RefSeq Size: 6254 bp
• RefSeq ORF: 2214 bp
• Locus ID: 18754
• UniProt ID: P16054
• Cytogenetics: 17 E4
• Gene Summary: Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. Mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts. Phosphorylates MARCKS, which phosphorylates and activates PTK2/FAK, leading to the spread of cardiomyocytes. Involved in the control of the directional transport of ITGB1 in mesenchymal cells by phosphorylating vimentin (VIM), an intermediate filament (IF) protein. In epithelial cells, associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates cell-cell contact. Phosphorylates IQGAP1, which binds to CDC42, mediating epithelial cell-cell detachment prior to migration. During cytokinesis, forms a complex with YWHAB, which is crucial for daughter cell separation, and facilitates abscission by a mechanism which may implicate the regulation of RHOA. In cardiac myocytes, regulates myofilament function and excitation coupling at the Z-lines, where it is indirectly associated with F-actin via interaction with COPB1. During endothelin-induced cardiomyocyte hypertrophy, mediates activation of PTK2/FAK, which is critical for cardiomyocyte survival and regulation of sarcomere length. Plays a role in the pathogenesis of dilated cardiomyopathy via persistent phosphorylation of troponin I (TNNI3). Involved in nerve growth factor (NFG)-induced neurite outgrowth and neuron morphological change independently of its kinase activity, by inhibition of RHOA pathway, activation of CDC42 and cytoskeletal rearrangement. May be involved in presynaptic facilitation by mediating phorbol ester-induced synaptic potentiation. Phosphorylates gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2), which reduces the response of GABA receptors to ethanol and benzodiazepines and may mediate acute tolerance to the intoxicating effects of ethanol. Upon PMA treatment, phosphorylates the capsaicin- and heat-activated cation channel TRPV1, which is required for bradykinin-induced sensitization of the heat response in nociceptive neurons. Is able to form a complex with PDLIM5 and N-type calcium channel, and may enhance channel activities and potentiates fast synaptic transmission by phosphorylating the pore-forming alpha subunit CACNA1B (CaV2.2). Downstream of TLR4, plays an important role in the lipopolysaccharide (LPS)-induced immune response by phosphorylating and activating TICAM2/TRAM, which in turn activates the transcription factor IRF3 and subsequent cytokines production. In differentiating erythroid progenitors, is regulated by EPO and controls the protection against the TNFSF10/TRAIL-mediated apoptosis, via BCL2. May be involved in the regulation of the insulin-induced phosphorylation and activation of AKT1.[UniProtKB/Swiss-Prot Function]