Sh3rf2 (NM_001146299) Mouse Untagged Clone

CAT#: MC221203

Sh3rf2 (untagged) - Mouse SH3 domain containing ring finger 2 (Sh3rf2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sh3rf2
• Synonyms: 2310046K19; 9130023G24Rik; AI452083; Ppp1r39; RNF158
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221203 representing NM_001146299
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGATTTGACGTTACTTGATCTCCTGGAGTGCCCTGTGTGCTTTGAAAAGCTCGATGTCACAGCCA
  AAGTTCTCCCCTGCCAGCACACCTTCTGCAAACCATGTCTACAGAGGATTTTCAAGGCCCACAAGGAGCT
  GAGGTGCCCGGAATGCAGGACTCTGGTGTTCTGCAGCATCGAGGCTCTGCCAGCCAACCTGTTGCTCGTG
  CGTTTGCTGGATGGTGTGCGTTCCGGACAGAGCTCGTGGAAAGGAGGCTCCTTCCGAAGGCCTCGGATAC
  TGACCTTGCAGGACAACAGGAAAGCCAAGAGCAGCCCCAGAAGTCTACAGGCTAGCCCTTTCCGGCTGGT
  GCCCAGTGTCAGGATCCACATGGATGGGGTGCCTCGAGCCAAGGCCTTGTGCAACTACAGAGGGAAGAAT
  CCTGGCGACCTGAAGTTTAATAAAGGCGATGTCATCCTCCTTCGGAGACAGCTTGATGAGAACTGGTACC
  AGGGGGAGATTAATGGCGTCAGCGGGATCTTCCCGGCCAGCTCTGTGGAAGTCATCAAACAGCTGCCCCA
  ACCACCCCCACTCTGCAGAGCCCTCTACAACTTTGACCTTCGAGACAAGGATAAGAGTGAGAACCAGGAC
  TGCCTGACCTTCCTCAAGGATGATGTCATCACAGTGATCAGCCGTGTAGATGAGAACTGGGCAGAGGGAA
  AGCTCGGAGATAAAGTGGGCATCTTCCCCATCTTGTTTGTAGAGCCAAACGTCTCGGCAAGACACCTCCT
  AGAGAACAAAGGACATCAGTTATCACGCACAAGACACCTGTCCCTGATGTCCTCCCCATCCAGAGGCAAA
  GCGACCAACACATCCTCGCTCCGAAAGAGCCCAGGGTCCAGGAGGAAGGGATCTGGGCAGTTCTCCATGA
  CTACAGCTTTGAACACACTCAACCGTACAGTCCATTCTCCTGAAGGCCACCAGATGGTGGAAATCAGCAC
  CCCGATGCTCATCAGCTCTAACAGCCCCTCTGTGCTCACCCAACATGGGGACAAGGCAGACTTCCCTACC
  AGCTCTACAGGACAGGTCAGCAGTTCTCAGCCTGCACCTGCCTCCCCGGGACATTCCACAGCCATGGTCA
  GTGTGCCCAGCTCCCAGCAACACCTTTCAAATAACATGTTTGTAGCCCTACACACTTACTCAGCCCATAG
  ACCCGAAGAGCTGGACCTGCAGAAGGGAGAAGGCATCAGGGTTCTGGGCAAGTACCAGGATGGCTGGCTG
  AAGGGCCTCTCCTTACTCACTGGACGAACTGGCATCTTTCCAAGCGACTATGTCATCCCAGTCTTCAGTT
  CAACAGCTAGAAAGACATCTAGTTTTCCTGATTCCCGGAGCCCCACTGTCTGCACCACATGGGCGTTATC
  CACCTCCTCTGTGTCCTCCCAGGGTAGCTTTGCAGAAGGTGATCCCCGGCAAAGTGGACCCTTCAAATCT
  GTCTTTGTGCCCACTGCCGTAGTTAACCCTTCGAGGAGCACACCTGGCCCAGGGTCTTCTGGACAAGGGT
  CTCTTAGGAAAGTGCGGAGCAGCATGAGAAAAAATGGATCCCTGCAGAGACCAGTTCAATCTGGGATCCC
  CACTTTCATGGTGGGCTCCCTCAGGTGCAGTCCTACCATGGTGATCCGGCCTCAGAGGTTCCAGTTTTAT
  CCGCCACAGGGGATGACCCCTTCCCCGACACCGATAATGGTGGAGATGGGGTCTAAATCTATTTACACTG
  GGGAGCCTGCCCTGACCTGCATCAACAGAGGTAGCAAGACCAGGATCCACTCAGCAGGCAATTCCATCAT
  CATGGAAGGCAAAGAAACGCCCATCAAGAGTGAACCGCCACCCAAGCCGCCTGCATCTGCGCCACCCTCC
  ATCCTCGTGAAACCAGAAAATTCAAAGAATGGCACTGAAAAGCAAGTCAAAACCGTGAGATTTCAGAATT
  ACAGCCCTCCGCCCACCAAACACTCTGCCTCCAGTCCCACCTCTGGAAAGCACGACCATCCAGCCACCTT
  GAAGGGGTCCCAGCATGAAGCAGTCTCCTCGGGAGGAGAGATGACCATTCTATTTGCCCACCGAAGTGGC
  TGCCACTCCGGGCAACAAACAGACCTTCGGAGAAAGTCAGCCTTTGGCAAGACCATGCCCTTATTGTCCA
  CTGCTTCAGCCACACAGACCTTGTTTCCCAGCAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001146299
• Insert Size: 2208 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146299.1, NP_001139771.1
• RefSeq Size: 4784 bp
• RefSeq ORF: 2208 bp
• Locus ID: 269016
• UniProt ID: Q8BZT2
• Cytogenetics: 18 B3
• Gene Summary: Has E3 ubiquitin-protein ligase activity. Acts as an anti-apoptotic regulator of the JNK pathway by ubiquitinating and promoting the degradation of SH3RF1, a scaffold protein that is required for pro-apoptotic JNK activation. Facilitates TNF-alpha-mediated recruitment of adapter proteins TRADD and RIPK1 to TNFRSF1A and regulates PAK4 protein stability via inhibition of its ubiquitin-mediated proteasomal degradation. Inhibits PPP1CA phosphatase activity (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.