Dtl (NM_029766) Mouse Untagged Clone

CAT#: MC221182

Dtl (untagged) - Mouse denticleless homolog (Drosophila) (Dtl), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dtl
• Synonyms: 2810047L02Rik; 5730564G15Rik; L2dtl; Ramp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221182 representing NM_029766
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTTCAACTCGGTACTCCGCCAGCCGCAGCTCGGCGTCCTGAGGAACGGGTGGTCTTCACATTACC
  CTCTACAATCCCTTCTAAGTGGTTATCAGTGCAACTGTAACGATGAACACACGTCTTATGGAGAAACAGG
  AGTCCCAGTTCCTCCTTTTGGATGCACCTTCTGTACCGCTCCCAGTATGGAGCATATATTAGCAGTTGCT
  AATGAAGAAGGCTTTGTCAGATTATATAATACAGAATCACAAACTAGCAAAAAGACATGCTTCAAGGAGT
  GGATGGCTCACTGGAATGCTGTCTTTGACTTGGCCTGGGTCCCTGGTGAACTTAAACTTGTTACAGCAGC
  CGGTGATCAGACAGCCAAATTTTGGGATGTAAGAGCTGGTGAGCTGATGGGGACATGCAAAGGCCACCAG
  TGCAGCCTCAAGTCTGTAGCCTTTCCCAAGTTTCAAAAAGCTGTGTTCTCTACAGGGGGGAGAGACGGCA
  ACATTATGATCTGGGACACCAGGTGTAACAAAAAAGATGGATTTTATAGACAAGTGAATCAAATCAGTGG
  AGCTCACAATACTGCAGACAAGCAAACCCCTTCAAAGCCCAAGAAGAAACAAAATTCAAAAGGACTTGCT
  CCTGCTGTGGATTCCCAGCAGAGTGTTACTGTGGTCCTCTTTCAGGATGAGAATACATTAGTCTCAGCAG
  GAGCCGTGGATGGAATAATCAAAGTATGGGATTTGCGCAAGAATTACACTGCTTATCGACAAGAACCCAT
  AGCATCCAAGTCTTTCCTGTACCCAGGTACCAGCACTCGAAAGCTAGGATACTCGAGTTTGGTTTTAGAC
  TCTACTGGCTCTACTTTATTTGCTAACTGCACAGATGACAACATCTATATGTTCAATATGACTGGCTTAA
  AGACTTCTCCGGTGGCTGTCTTCAATGGACACCAGAACTCTACCTTTTATGTAAAATCAAGTCTTAGTCC
  AGATGACCAGTTTTTAATCAGTGGTTCAAGTGATGAAGCTGCCTACATTTGGAAGGTTTCCATGCCATGG
  CATCCTCCTACTGTGCTCCTGGGTCATTCTCAAGAGGTCACGTCTGTGTGCTGGTGTCCATCAGACTTCA
  CCAAGATTGCAACCTGCTCTGATGATAATACACTGAAAATCTGGCGCTTGAATAGAGGCCTAGAGGAGAA
  ACCAGGTGATAAACATTCCATAGTGGGTTGGACCTCTCAGAAGAAAAAAGAAGTGAAAGCCTGCCCAGTA
  ACGGTACCAAGTAGCCAGAGTACTCCTGCCAAAGCTCCCAGAGCCAAGAGCAGTCCATCCATCTCCTCTC
  CTTCGTCAGCAGCTTGTACTCCGAGCTGTGCAGGAGACCTCCCTCTTCCTTCAAGTACCCCCACATTCTC
  AGTCAAAACCACTCCTGCCACGACCCGTTCTTCAGTCAGCAGAAGAGGCTCCATCTCTTCTGTGTCTCCC
  AAGCCACTCTCATCTTTCAAGATGTCGCTTAGAAACTGGGTGACCCGAACACCTTCCTCATCACCACCTG
  TCACTCCACCTGCTTCTGAGACAAAGATCTCATCTCCAAGAAAAGCTCTTATTCCTGTGAGCCAGAAGTC
  ATCACAGGCAGATGCTTGCTCTGAATCTAGAAATAGAGTGAAGAGGCGTCTTGACTCAAGCTGTCTGGAG
  AGTGTGAAACAAAAGTGTGTGAAGAGTTGCAACTGTGTCACTGAGCTTGACGGCCAAGCGGAGAGTCTTC
  GTTTGGATCTGTGCTGCCTTTCCGGCACCCAGGAAGTCCTTAGCCAAGACTCCGAGGGTCCTACCAAATC
  AAGCAAGACTGAAGGTGCTGGCACAAGCATCTCAGAACCTCCTTCTCCTGTCAGTCCTTATGCTTCTGAA
  GGCTGTGGACCACTGCCTCTTCCTTTGAGACCTTGTGGAGAAGGATCTGAGATGGTGGGCAAAGAGAATA
  GCTCTCCAGAGAATAAGAACTGGTTGTTGGCCATAGCAGCCAAACGCAAGGCAGAAAATTCATCCCCAAG
  AAGTCCATCATCTCAGACACCCAGTTCCAGGAGACAAAGTGGGAAGACGTCACCAGGCCCGGTCACCATT
  ACTCCCAGCTCCATGAGGAAGATATGTACATACTTTCGTAGAAAGACTCAAGATGACTTCTGCAGTCCTG
  AACACTCAACTGAATTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029766
• Insert Size: 2190 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029766.3, NP_084042.1
• RefSeq Size: 4221 bp
• RefSeq ORF: 2190 bp
• Locus ID: 76843
• UniProt ID: Q3TLR7
• Cytogenetics: 1 H6
• Gene Summary: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1), FBH1, KMT5A and SDE2. CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis. The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).