Pwwp2a (NM_027557) Mouse Untagged Clone

CAT#: MC221173

Pwwp2a (untagged) - Mouse PWWP domain containing 2A (Pwwp2a), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pwwp2a
• Synonyms: 4631424J17Rik; AI891583; AU024105; D930040F23Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221173 representing NM_027557
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGTGGCTGCTGAGGCGGCGGCGACCGCGGCGTCCCCCGGGGAAGGGGGCGCCGGCGAGGCCG
  AGCCGGAGCTAGAGCCCATTCCCGGCAGCGAAGCCGGCACCCCCCTCCCGGTCACGGCCACGGAGGCGGC
  GGTGCCGGACGGCGAGGCCGACGGGCGCCAGTCCGCCCCTCAGGCCGACGAGCAGCCGCTGCCGCCACCG
  CCGCCGCCGCCGCCGCCGGGGGAGCTGGCCGATAGCTCCGAGGCTGAGGAGGCGAAGCCGCCCGAGCCCG
  CGGCTGTCCCGGTCTCGCCGCCCGAGCAGCCTCCGGCCGCCCCCGAGCAGCCCGAGGACGCGCCGCGGCC
  GCCACCAGCTCCGGCGCTCGTGCCGCCGGCGGGCGGGGACTCGGCGGTGTCGCACCTCATCCCCGGCTCG
  GAGGTGCGGGTCACGCTGGACCACATCATCGAGGACGCGCTCGTCGTGTCGTTCCGCCTCGGGGAGAAGC
  TCTTCTCCGGGGTCCTCATGGACCTATCCAAAAGGTTTGGGCCCCATGGGATCCCTGTGACAGTATTTCC
  CAAAAGGGAATATAAGGACAAACCGGACGCCATGCAGCTCCAAAGTACCACATTCCAAGAAGGGATAGAA
  GTCAAGCAGGAAGTGAACGGTGCTGTTCCCGATGACCTCTCTCCAGTCCCTCCTCCTGAGCGCCTGTGGG
  CTTCCAAGCCACCTCCTCTCTTCCATGAAGGAGCACCTTACCCTCCCCCCTTGTTTATCAGGGACACATA
  TAACCAATCAATACCTCAGCCGCCGCCTCGGAAAATTAAGCGACCCAAACGAAAAATGTACAGGGAAGAA
  CCCACTTCAATAATGAATGCGATTAAGCTACGGCCCAGGCAAGTCCTGTGTGATAAGTGTAAAAACAGTG
  TTGTTGCTGAAAAGAAGGAAATTAGGAAAGGTAGCAGTGACTCTTCTAGGTATGAAGATAAAAAACGGAG
  AAATGACAGTGTAGCTACTGTGAACAAAAAACTGAAAACTGACCATAAAGTGGATGGGAAAAACCAAAAC
  GAAAGCCAGAGAAGGAACACTGTGGTGAGGGTTTCCAGTATCGCTCACAGCAGGGGCAGAGTAGTCAAGG
  TTTCTGCTCAGGCAAATACATCAAAAGCTCAGTTAAATACCAAGAAAGTGCTCCAAAGCAAGAACATGGA
  TCACGCAAAAGCTCGGGAAGTATTGAAAATTGCCAAAGAAAAGGCACAGAAGAAGCAAAGTGAAACCTCC
  ACTTCCAAAACCGCCCATGCGAAAGTCCACTTCACACGTCGGTATCAGAGTCCCAGCTCAGGTTCTCTCC
  CACCCCGAGTGCGGTTAAAACCACAAAGGTACAGGAACGAAGAAAATGACTCTTCTCTGAAGACGGGACT
  GGAGAAAATTCGGAGTGGCAAGCTGGCGCCTAAGCCGCAGTCTCGATGCACCTCCACCCGCTCAGCAGGT
  GAGGCCCCTTCAGAAAAACCGAGTCCCTCAGAAGGCCCGGAAGAGTCCGCCGGTGAGGTTCAGGACACAA
  GCAGAGTGCGTGTGCCTGGTGAGCAGGAGGAACTGCGGATGTTGGGCAAAAAGGGCAGCAAAAGCAGTAT
  CTCTGTTTACCTGACCCTAAATCAGGAGACATCTGACTCTTCCAGTGCCTCAGTGTGTAGCATTGATAGC
  ATGGATGATTTGAAATCCTCCAACTCTGAGTGTAGCTCTTCTGAGAGCTTCGTTTTTCCTCCCGGCTGTA
  TGCACGCACCTTCTGCCTCTTCCACTTCTACTTCCTTCTCTTCAAAGGAAGAGAATAGCCTCCGTAATTC
  TTTGAAAATGAAAATCTTTTCAAAAAATGTCTCTAAATGTATCACACCAGATGGCAGGACCATATGTGTA
  GGGGACATTGTTTGGGCCAAGATATATGGCTTCCCTTGGTGGCCAGCCCGTATTCTTACCATCACTGTAA
  GCCGGAAAGATAACGGCCTTTTAGCCCGGCAGGAGGCCCGTATCTCATGGTTTGCATCTCCAACCACATC
  TTCCCTTGCTCTCTCACAACTCTCCCCCTTTCTAGAAAACTTCCAGTTACGGTTTAATAAGAAGAGAAAG
  GGTCTGTATCGCAGGGCTATCACAGAGGCTGCTAAGGCTGCTAAGCAGCTGACCCCTGAAGTGCGGGCTT
  TGTTGACACAATTTGAAACGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027557
• Insert Size: 2193 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027557.1, NP_081833.1
• RefSeq Size: 3295 bp
• RefSeq ORF: 2193 bp
• Locus ID: 70802
• UniProt ID: Q69Z61
• Cytogenetics: 11 B1.1
• Gene Summary: H2A.Z-specific chromatin binding protein which may play an important role in the neural crest stem cell migration and differentiation during early development. Also required for proper mitosis progression.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (b) has a longer and distinct C-terminus, compared to isoform a.