Ppp1r3f (NM_138605) Mouse Untagged Clone

CAT#: MC221168

Ppp1r3f (untagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 3F (Ppp1r3f), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ppp1r3f
• Synonyms: DXImx48e; R3F; RF3; Sfc15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221168 representing NM_138605
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCGCACGGCCCCTGTGGAGCCACCGCTGCGGCATCCCGCGCCCCCGTCGCCGGCTGCGGGAGAGC
  CCCGCGCCTCGGCTGAGGCGGCGGTGGCCCCGCGGAGGGTGCTGTTCGCTGACGAGGCCCTGGGGCTGCC
  TCTGGCGCAGCTACGCCGCTACCGGCCGTGGGGCGGGCCCGGGGCGGGCAAGATGGCGGCGGCGACCGGG
  CAAGATGGCGGCGGTGGCGGGGCTGATGAGGAAGACGATGGCGAGGATGGGGATGAAGGGGAGGAGGAAG
  AGGAGGCTTTTCCCGATCCCTCTCCGCCGTGCCCCGTTCCCGCTGGCGGGGGGTTTTACCTGGTGCCCAC
  ATTTTCGTTGCCGCCCGCGCTGGGCCGTCTGGAGCGGTTGGGGCGCGTGATGGTGGAGTTGGAGGCGCTG
  CTGCCGCCTCCTGGAGCGGTTCCCGGGGGTTCCGGGGTGTGGGTGCCTGGGGGACGCCCGCCAGTGGTGC
  GCGGGTTGGTGCGCGTGCTGAACCGCTCCTTCGAGAAGGCGGTGCACGTGCGGGCCTCACACGACGGTTG
  GGCTACCTTCTGCGACCACCCAGCCCGCTATGTCCCACGCAGCCCGCCTGGGGCAGGAGTGGGAGGAACA
  GGAGCAGGAGATCCCCTCCTGGATCCCGGGCTAGGCCTGGGCCCTGGCCAGATGTCAGCCTCCTCACCCG
  ACGACGGTGGCTGCACTGACCGCTTTGCCTTCCAGCTGCCCTTTGCTGAGGGCGCGAGCGATGGGGCGCG
  CCTCGACTTCGTGGTGCGCTATGAGACCCCCGAGGGCACTTTCTGGGCCAACAACCATGGCCGCAACTAC
  ACTGTCCTGCTCCGGATCGCACCCGCTCCCACACCCACTGATGCCGAAGGGCTGCCCCAGCAGCAGCAGC
  TGCAGCAGCTGGAGCCACAGCCTGAGTGCCAGGGTCCTGTGGAGGCTGAGGCCAGGCAGCTGAAGAGCTG
  CATGAAGCCGGTGAGGCGCAGGCCTTTTGAAGAAGAGCCAAGGATGAGGAGTGCGGACGATAATACCCTA
  GCAGAACATCCTGATGTTCGAGAGTCACTAGGTCCCCTGTTGGCCCCTACCCCTCTCCGTCCATGGCCCC
  AGATGACACTTCAGGTGCCCGAAGTTATGTTGACCAGCAACCCTCAAGAAGAAGGTGACATCCCCAGAAG
  CAACCCACCTGTGGCTTTTACAGAGGTCCGCCAGGCACCTGCCATCAGGATTCTTCCAGCAACCTGTGGC
  CTGGGTGGCCCCCCAAGGGACCAGGCCTCAGGGCCCGATGCAAGTGATAGAGCTGCTGGCTCCTTCCTGG
  AACCCACCCAGCAGCAGGTGGAGGCTGCGTGGGAAAGTGGAGGGGGTCGAAAGGCCCCTATGGTAGGGGC
  TCTGACAGATGAGCCCGCCAGGGGTCTGGAGATTGTGAGTGGCTTGGATGAGTTGCTTGGTGAAGACACC
  ATTGACCAGGAGTTGGAGCAGCTCTACCTGTCTCACCTGAGCCGCTTGAGAGCTGTGGCAGCGGCGGGTG
  GCGGGGAAGGCACTTCCCCCACTCATGCCCTGGGCATACTCACAGACCGTGACCTGATCTTGAAGTGGCC
  TGGCCCCGAGAGGGCCCTGAACAGTGCTCTGGCCGAGGAGATCACGCTGCACTATGCGCGCCTGGGCTGT
  GGCGTGGAGCTCATCAAGGACACTGAAGACCCAGATGACGAGGGGGAGGGTGAAGATGGGCTCTCCATCA
  CTCCTTCTAGCCCTGAAGGGGGCAGCCCTAAGGAATCGCCTCCAGAAATCCTCTCTGGAGCCCGCTCTGT
  GATAGCTACTATGGGAGATGTGTGGGTTCCATGGGCAGAGCGCTCTAGCTCTAGATGTGATAGCCCAGTG
  GTTCTGGGAACACAGGGTCAGTTCACTGAGAATCCAGAGAAAGGGATGGGCAAGGACACCAAGTCTCTGC
  ACCTGAATAGGGTAATAGTTGGGATGAGCAAGTCCCCTGGGGAGGCTGGGACGGAAAGCCAGATGGAGGA
  GCTACCTACTGAGCGGGAAAGCAGCTGGGTACCTAGTTCTGAGAAAGAGCTGCCTCTACCAGTCCAGCAA
  GAGCAGAGTCCTGCCCTCCTTGGTCCCACAGGGACTGAAGTCTGTCTGTCTAGTGTAGCCAAGCCTCATG
  TGAACTCCCAGGAAGAAGAGGGTGGAAGCCTGAATCTTGAGTCCCCAAAGAGGTCTCCCATGCCAGCGGC
  CCCTGCAGAATGTGCGTGTGGGTTGGCTCCTCAGCTCTGGGGGCCCTTGACCCAAACTTTGGGTGTACTG
  GCTGGGCTCGTTATGGTCCCCGTGGCTTTGAACAGCGGTGTGTCCCTTCTGGTGCTGGTGCTGTGCCTCT
  CTCTGGCCTGGTTCTCGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_138605
• Insert Size: 2400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138605.3, NP_613071.3
• RefSeq Size: 3489 bp
• RefSeq ORF: 2400 bp
• Locus ID: 54646
• UniProt ID: Q9JIG4
• Cytogenetics: X 3.4 cM
• Gene Summary: Glycogen-targeting subunit for protein phosphatase 1 (PP1).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.