Ubc (NM_019639) Mouse Untagged Clone

CAT#: MC221162

Ubc (untagged) - Mouse ubiquitin C (Ubc), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ubc
• Synonyms: 2700054O04Rik; AI194771; Rps27a; TI-225; Uba52; Ubb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221162 representing NM_019639
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGATCTTTGTGAAAACCTTAACTGGTAAGACCATCACCCTGGAGGTCGAGCCCAGTGACACCATTG
  AGAATGTCAAGGCAAAGATCCAGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGG
  CAAGCAGCTGGAAGATGGCCGCACCCTGTCAGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTC
  CTTCGCCTCAGAGGTGGCATGCAGATCTTTGTGAAGACCCTGACAGGCAAGACCATCACCCTGGAGGTCG
  AGCCCAGTGACACCATAGAGAATGTCAAGGCAAAGATCCAGGACAAGGAGGGCATCCCCCCTGACCAGCA
  GAGGCTGATCTTTGCAGGCAAGCAGCTGGAAGATGGCCGCACCCTGTCAGACTACAACATCCAGAAAGAG
  TCCACCCTGCACCTGGTCCTTCGCCTCAGAGGTGGGATGCAGATCTTTGTGAAGACCCTGACAGGCAAGA
  CCATCACCCTGGAGGTCGAGCCCAGTGACACCATAGAGAATGTCAAGGCAAAGATCCAGGACAAGGAGGG
  CATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGCAAGCAGCTGGAAGATGGCCGCACCCTGTCAGAC
  TACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTTCGCCTCAGAGGTGGCATGCAGATCTTTGTGA
  AGACCCTGACAGGCAAGACCATCACCCTGGAGGTCGAGCCCAGTGACACCATTGAGAATGTCAAGGCAAA
  GATCCAGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGCAAGCAGCTGGAAGAT
  GGCCGCACCCTGTCAGACTACAACATCCAGAAAGAGTCCACCCTGCACTTAGTCCTTCGCCTCAGAGGTG
  GGATGCAGATCTTTGTGAAGACCCTGACAGGCAAAACCATCACCCTGGAGGTCGAGCCCAGTGACACCAT
  TGAGAATGTCAAGGCAAAGATCCAGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCA
  GGCAAGCAGCTGGAAGATGGCCGCACCCTGTCAGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGG
  TCCTTCGCCTCAGAGGTGGGATGCAGATCTTTGTGAAGACCCTGACAGGCAAGACCATCACCCTGGAGGT
  CGAGCCCAGTGACACCATAGAGAATGTCAAGGCAAAGATCCAGGACAAGGAGGGCATCCCCCCTGACCAG
  CAGAGGCTGATCTTTGCAGGCAAGCAGCTGGAAGATGGCCGCACCCTGTCAGACTACAACATCCAGAAAG
  AGTCCACCCTGCACCTGGTCCTTCGCCTCAGAGGTGGCATGCAGATCTTTGTGAAGACCCTGACAGGCAA
  GACCATCACCCTGGAGGTCGAGCCCAGTGACACCATAGAGAATGTCAAGGCAAAGATCCAGGACAAGGAG
  GGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGCAAGCAGCTGGAAGATGGCCGCACCCTGTCAG
  ACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTTCGCCTCAGAGGTGGGATGCAGATCTTTGT
  GAAGACCCTGACAGGCAAGACCATCACCCTGGAGGTCGAGCCCAGTGACACCATAGAGAATGTCAAGGCA
  AAGATCCAGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGCAAGCAGCTGGAAG
  ATGGCCGCACCCTGTCAGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTTCGCCTCAGAGG
  TGGCATGCAGATCTTTGTGAAGACCCTGACAGGCAAGACCATCACCCTGGAGGTCGAGCCCAGTGACACC
  ATAGAGAATGTCAAGGCAAAGATCCAGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTG
  CAGGCAAGCAGCTGGAAGATGGCCGCACCCTGTCAGACTACAACATCCAGAAAGAGTCCACCCTGCACCT
  GGTCCTTCGCCTCAGAGGTGGCATGCAGATCTTTGTGAAGACCCTGACAGGCAAGACCATCACCCTGGAC
  GTCGAGCCCAGTGTTACCACCAAGAAGGTCAAACAGGAAGACAGACGTACCTTCCTCACCACAGTATCTA
  AAAAGAGCCCTCCTTGTGCTTGTTCTTGGGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019639
• Insert Size: 2205 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019639.4, NP_062613.3
• RefSeq Size: 2424 bp
• RefSeq ORF: 2205 bp
• Locus ID: 22190
• UniProt ID: P0CG50
• Cytogenetics: 5 64.18 cM
• Gene Summary: Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.[UniProtKB/Swiss-Prot Function]