Mcm5 (NM_008566) Mouse Untagged Clone

CAT#: MC221157

Mcm5 (untagged) - Mouse minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisiae) (Mcm5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mcm5
• Synonyms: AA617332; AI324988; AL033333; Cdc46; Mcmd5; P1-CDC46
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221157 representing NM_008566
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGGCTTCGACGACCCGGGCATTTTCTACAGCGACAGCTTCGGTGGCGACCCCGGTGCGGAAGAGG
  GCCAGGCCCGCAAGTCGCAACTGCAGAGGCGATTCAAGGAGTTCCTGAGACAGTACCGAGTGGGCACCGA
  TCGCACGGGCTTCACCTTCAAGTACAGAGATGAACTCAAGCGGCATTACAACCTGGGTGAATACTGGATC
  GAGGTGGAGATGGAGGACCTGGCCAGTTTTGACGAGGAACTGGCTGACCACTTGCATAAACAGCCGGCCG
  AGCACTTACAGCTGCTTGAGGAAGCTGCCAAGGAGGTGGCAGATGAGGTGACCCGGCCCCGGCCAGCTGG
  AGATGAGCTGCTCCAAGACATCCAGGTCATGCTCAAGTCAGATGCCAGCCCGTCGAGCATTCGGATTCTG
  AAGTCAGACATGATGTCACACCTGGTGAAGATCCCTGGCATCATCATTTCAGCCTCTGCAGTCCGTGCCA
  AGGCTACTCGTATCTCCATTCAGTGCCGCAGCTGCCACAATACCCTCACCAATATCGCCATGCGCCCAGG
  CCTAGAGGGCTATGCCCTTCCCAGGAAGTGCAATATGGATCAGGCTGGGCGCCCAAAGTGCCCACTGGAT
  CCATACTTCATCATGCCTGACAAGTGCAAGTGTGTGGACTTCCAGACTCTGAAACTGCAGGAGCTGCCTG
  ATGCAGTCCCTCATGGTGAGATGCCCAGGCACATGCAGCTTTATTGTGACAGGTACCTGTGTGACAAGGT
  TGTTCCTGGGAACAGGGTCACCATCATGGGCATTTATTCCATCAAGAAGTTTGGCTTGAACCCCAGCAAG
  GGCCGGGACAGGGTAGGTGTGGGCATCCGGAGCTCGTACATCCGAGTGCTGGGCATCCAGGTGGACACAG
  ATGGCTCTGGCCGAAGCTTTGCTGGGTCTGTCAGCCCACAGGAAGAGGAGGAATTTCGTCGCCTGGCTGC
  CCTCCCCAACATATATGAGCTCATCTCCAAGAGCATTTCCCCCTCCATCTTTGGGGGCATGGATATGAAG
  AAGGCCATTGCCTGCCTGCTTTTTGGGGGTTCCCGGAAGAGGCTCCCAGATGGACTCACTCGCCGAGGTG
  ATATCAACTTGCTGATGTTGGGAGACCCTGGTACAGCCAAGTCTCAGCTTCTGAAGTTTGTGGAGAAGTG
  CTCTCCCATTGGGGTGTACACATCTGGGAAAGGTAGCAGTGCTGCTGGCTTGACTGCCTCAGTGATACGG
  GACCCCTCATCTCGAAACTTCATCATGGAAGGTGGAGCCATGGTTCTGGCCGATGGTGGGGTTGTCTGTA
  TTGATGAGTTTGACAAGATGCGGGAAGATGACCGTGTTGCAATCCATGAGGCTATGGAGCAGCAGACCAT
  CTCCATTGCTAAAGCTGGGATCACTACCACCTTGAACTCTCGCTGCTCTGTTCTGGCTGCAGCCAACTCA
  GTGTTTGGCCGATGGGATGAGACAAAAGGGGAGGACAATATTGACTTCATGCCTACCATCTTGTCCCGAT
  TTGATATGATCTTCATCGTCAAAGATGAGCACAATGAGGAGAGGGACATGATGCTAGCCAAACATGTGAT
  GACTCTGCATGTGAGTGCACTGACACAGACACAGGCTGTGGAGGGTGAGATCGACCTGGCCAAGATGAAG
  AAGTTCATTGCCTACTGCCGAGCGAGGTGTGGACCTCGGCTATCAGCAGAGGCAGCAGAGAAGCTGAAAA
  ACCGCTACATCATCATGCGGAGTGGGGCTCGTCAGCATGAGAGGGACAGTGACCGGCGTTCCAGCATCCC
  CATCACTGTGCGGCAGCTGGAGGCTATTGTGCGCATTGCTGAGGCCCTCAGTAAGATGAAACTGCAGCCC
  TTTGCCACTGAGGCTGATGTAGAGGAGGCATTGAGACTGTTCCAGGTGTCCACACTGGATGCTGCTTTGT
  CTGGCAATCTGTCGGGGGTGGAGGGCTTCACTACCCAGGAGGACCAGGAGATGCTGAGCCGCATTGAGAA
  GCAACTCAAGCGCCGTTTTGCCATTGGCTCTCAGGTGTCTGAACACAGCATTGTCCAGGACTTCACCAAA
  CAGAAATATCCAGAGCACGCTATCCGAAAGGTGCTGCAGCTCATGCTACGCAGGGGTGAGATCCAACACC
  GTATGCAGCGCAAGGTGCTCTATCGCCTCAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008566
• Insert Size: 2205 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008566.3, NP_032592.2
• RefSeq Size: 3441 bp
• RefSeq ORF: 2205 bp
• Locus ID: 17218
• UniProt ID: P49718
• Cytogenetics: 8 C1
• Gene Summary: The protein encoded by this gene is a member of the oligameric minichromosome maintenance protein complex. During DNA replication, the complex loads onto chromatin in early G1 and is converted into an active replicative helicase during S phase. It functions to limit DNA synthesis to once per cell cycle. During embryogenesis, the encoded protein is negatively regulated through expression of paired box protein Pax 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.