Capn3 (NM_001177799) Mouse Untagged Clone

CAT#: MC221149

Capn3 (untagged) - Mouse calpain 3 (Capn3), transcript variant c, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Capn3
• Synonyms: AI323605; Capa-3; Capa3; Lp82; p94
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221149 representing NM_001177799
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAACTGTTATTAGTCCAACTGTGGCCCCAAGGACGGGGGCTGAGCCCAGGTCCCCAGGGCCAGTTC
  CTCACCCAGCTCAAGGCAAGACCACCGAGGCTGGAGGTGGACACCCAAGTGGCATCTATTCGGCCATCAT
  TAGCCGCAATTTTCCGATCATCGGTGTGAAAGAGAAGACATTTGAGCAGCTCCGCAGGAAGTGCCTAGAG
  AAGAAAGTTCTTTATCTAGACCCCGAGTTCCCACCGGATGAGACCTCTCTCTTTTACAGTCAGAAGTTCC
  CCATCCAGTTCGTCTGGAAGAGACCTCCGGAAATTTGTGAGAATCCCCGGTTTATCATTGGTGGAGCCAA
  CAGGACTGACATCTGCCAAGGAGATCTAGGGGACTGCTGGTTTCTTGCAGCCATCGCCTGCCTGACCCTG
  AATGAGCGGCTGCTTTTCCGAGTTATACCCCATGATCAAAGTTTCACTGAAAACTACGCAGGGATCTTCC
  ACTTCCAGTTCTGGCGCTATGGAGACTGGGTAGATGTGGTCATTGACGACTGTCTGCCAACATACAACAA
  TCAGCTGGTTTTCACCAAATCCAACCACCGCAATGAGTTCTGGAGTGCTCTCCTTGAGAAGGCTTATGCC
  AAGCTCCATGGCTCCTATGAAGCTCTGAAAGGTGGGAATACCACAGAAGCCATGGAGGACTTCACAGGAG
  GGGTGACAGAGTTTTTTGAGATCAAGGATGCTCCGAGTGACATGTACAAGATTATGAGGAAAGCTATCGA
  GAGAGGCTCCCTCATGGGCTGCTCCATTGACACAATTGTTCCAGTGCAGTATGAAACAAGAATGGCCTGT
  GGGTTGGTGAAAGGGCATGCCTATTCAGTCACTGGGCTGGAGGAGGCCCTGTTCAAAGGTGAGAAGGTGA
  AGTTGGTGCGGCTGCGGAACCCCTGGGGCCAGGTGGAGTGGAACGGCTCTTGGAGTGATGGTTGGAAGGA
  CTGGAGCTTTGTAGACAAAGATGAGAAGGCCCGTCTACAGCACCAGGTTACTGAGGATGGAGAGTTCTGG
  ATGTCATATGATGACTTCGTCTACCATTTCACGAAGCTGGAGATCTGCAACCTCACAGCTGACGCCCTGG
  AGTCCGATAAGCTTCAGACCTGGACGGTGTCTGTAAACGAGGGCCGCTGGGTGAGGGGCTGTTCTGCTGG
  AGGCTGCCGAAACTTCCCAGACACTTTCTGGACCAACCCGCAGTACCGTCTCAAGCTTCTGGAGGAAGAC
  GATGACCCTGAGGACTCTGAGGTAATCTGCAGCTTCCTCGTGGCTCTGATGCAGAAGAACCGGCGCAAGG
  ACCGGAAGCTGGGGGCCAACCTCTTCACTATTGGCTTCGCCATCTACGAGGTTCCCAAAGAGATGCACGG
  GAATAAGCAACACCTGCAGAAGGACTTCTTCTTGTACAACGCCTCCAAGGCCAGAAGCAAAACCTACATC
  AACATGCGGGAGGTGTCCCAGCGCTTCCGCCTGCCACCCAGCGAGTATGTCATCGTACCCTCCACCTACG
  AGCCCCACCAGGAGGGGGAATTCATCCTCCGAGTCTTCTCCGAAAAGAGGAATCTCTCTGAGGAAGCTGA
  AAATACAATCTCTGTGGATCGGCCAGTGCCAAGGCCTGGCCACACAGACCAGGAAAGTGAGGAGCAGCAG
  CAATTCCGGAACATCTTCAGGCAGATCGCAGGCGACGACATGGAGATCTGTGCAGATGAACTCAAGAATG
  TCCTCAACACAGTGGTGAACAAACACAAGGACCTGAAGACACAAGGGTTCACGCTGGAGTCCTGCAGAAG
  CATGATAGCTCTCATGGATACAGATGGCTCTGGAAGACTGAATCTTCAAGAGTTCCATCACCTCTGGAAA
  AAGATCAAGGCCTGGCAGAAAATCTTCAAGCACTATGACACAGACCATTCCGGTACCATCAATAGCTATG
  AGATGCGAAATGCAGTCAATGATGCAGGCTTCCATCTCAACAGCCAACTCTATGACATCATCACCATGCG
  CTATGCGGACAAACACATGAACATCGACTTTGACAGCTTCATCTGCTGCTTCGTCAGGCTGGAAGGGATG
  TTCAGAGCTTTTAACGCATTTGACAAGGATGGCGATGGTATCATCAAACTGAATGTACTTGAGTGGCTGC
  AGCTTACCATGTATGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177799
• Insert Size: 2190 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177799.1, NP_001171270.1
• RefSeq Size: 2891 bp
• RefSeq ORF: 2190 bp
• Locus ID: 12335
• UniProt ID: Q64691
• Cytogenetics: 2 60.31 cM
• Gene Summary: Calcium-regulated non-lysosomal thiol-protease.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (c) lacks several alternate, in-frame coding exons, compared to variant a. The resulting protein (isoform c) is shorter when it is compared to isoform a.