Arap1 (NM_198096) Mouse Untagged Clone

CAT#: MC221103

Arap1 (untagged) - Mouse ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (Arap1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arap1
• Synonyms: 2410002L19Rik; Centd2; mKIAA0782
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221103 representing NM_198096
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTTCTACGGAATAATCAGAGTACAGAGGTCCCTCGGTTGGACTCAGTGAAGCCCTTGGAAAAGC
  ACTACTCCGTTACCCTGCCAACTGTGAGCCACAGCGGCTTCCTGTACAAAACTGCTTCTGCCGGCAAACC
  TCTGCAGGACCGCCGTGCCCGGGAAGAGTTCAGCCGGCGCTGGTGTGTCCTGAGTGATGGGGTCCTGAGC
  TACTATGAAAACGAACGGGCAGTGACACCCAATGGGGAGATTCGGGCCAGCGAGATAGTATGCCTAGCAG
  TTTCCCCTCTGGACACCCATGGCTTTGAGCACACCTTTGAGGTGTACACAGAGGGAGAACGGCTGTACCT
  GTTTGGGCTGGAGAATGCAGAGCTGGCTCATGAGTGGGTCAAGTGCATTGCCAAGGCGTTCGTGCCTCCC
  CTGGCTGAGGACCTGCTAGCCCGGGACTTTGAACGTCTTGGGCGCCTACCCTGTAAAGCTGGCCTGAGCC
  TTCAGCAGGCTCAGGAAGGCTGGTTTGCCTTGACTGGCTCTGAGCTCCGGGCTGTCTTCCCAGAGGGGCC
  CTGGGAAGAGCCGCTGCAGCTCCGGAAACTGCAAGAGCTTTCTATCCAAGGAGACAGCGAGAACCAAGTG
  CTGGTGCTGGTGGAGCGGAGGAGGACACTGTACATCCAGGGTGAGCGGCGGCTGGACTTCATGGCTTGGC
  TAGGGGTCATCCAGAAAGCAGCAGCCAGCTTGGGAGACACACTATCAGAACAACAGCTTGGGGACTCGGA
  CATCCCAGTGATTGTGTACCGCTGTGTGGACTACATCACGCAGTGTGGCTTGACCTCAGAGGGTATCTAT
  CGAAAGTGTGGTCAGACCTCCAAGACTCAGAGACTGCTAGACAGCCTCCGGCAGGACGCACGCTCTGTGC
  ACCTAAAGGAGGGAGAACAGCACGTGGACGACGTCTCCTCTGCACTCAAACGCTTCCTCAGGGACCTGCC
  CGATGGGCTCTTCACGCGTGCGCAGCGCCTGGCCTGGCTGGAGGCCTCTGAGATCGAGGATGAGGAAGAA
  AAGATCTCCAGGTATCGAGAGCTCTTGGTGCATCTGCCCCCTGTCAACCGGGCCACTGTGAAGGCCCTTA
  TCAGCCATCTGTACTGTGTACAGTGCTTCTCAGACACAAACCAAATGAACACACACAACCTGGCTATCGT
  GTTTGGGCCTACACTCTTCCAGACAGACGGGCAGGACTACAAGGCCGGCAAGGTGGTGGAAGACCTCATC
  AACCACTACGTGGTGGTGTTCAGTGTGGACGAGGAGGAGCTGAGGAAGCAGAGGGAGGAAGTCACGGCCA
  TCGTGAAGATGCGAGTGGCTGGCACTGCCAGTGGGACCCAGCATGCTGGCGACTTCATCTGCACAGTCTA
  CCTGGAGGAGAAGAAGGTGGAGACTGAACAGCATGTTAAGATCCCAGCATCCATGACTGCAGAGGAGCTT
  ACTCTGGAGATTCTGGACCGCCGCAATGTGAGCATCAGGGAGAAGGACTACTGGACTTGCTTTGAGGTCA
  ACGAGAAGGAGGAGGCAGAGCGCCCGCTGCACTTTGCAGAGAAGGTGCTGCCCATTGTCCATGGGCTGGG
  CATAGACAGCCATCTGGTGGTGAAGAAGTACCAGTCCATGGAGGCCATGCTGTTGTACTTGGCCAGCCGT
  GTGGGTGACACCAAGCATGGTATGATGAAGTTCCGTGAAGACCGCAGCCTCCTGGGTCTGGGGCTACCTT
  CGGGTGGCTTCCACGATCGCTACTTCATTCTCAACAGCAGCTGCCTACGGCTCTACAAGGAGGTCCGGAG
  TCACCGGCCTGAGAAGGAGTGGCCTGTCAAGAGCCTCAAAGTCTACCTGGGTGTAAAGAAGAAACTGCGG
  CCACCTACTTGCTGGGGCTTCACGGTGGTGCACGAGACAGAAAAGCACGAGAAGCAGCAGTGGTACCTCT
  GCTGTGACACGCAGATGGAACTTCGAGAGTGGTTTGCCACCTTCCTCTCTGTGCAGCACGATGGCCTGGT
  GTGGCCCTCCGAGCCATCTCGAGTGTCCCGGGCAGTGCCTGAGGTCCGGATGGGCAGTGTATCGCTGATC
  CCTCTACGAGGCAGTGAAAATGAAATGCGCCGGAGTGTGGCAGCCTTCACTGCTGACCCCCTTTCCCTTC
  TCCGCCATGTCTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_198096
• Insert Size: 2184 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198096.1, NP_932764.1
• RefSeq Size: 3673 bp
• RefSeq ORF: 2184 bp
• Locus ID: 69710
• UniProt ID: Q4LDD4
• Cytogenetics: 7 E2
• Gene Summary: Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks some 5' coding sequence, compared to variant 3. These differences cause translation initiation at a downstream AUG and an isoform (2) with a shorter N-terminus compared to isoform 3. Sequence Note: This RefSeq record was created using sequences from two different strains, C57BL/6J and FVB/N-3, because no single transcript from the same strain was available for the full length of the gene.