Brd4 (NM_198094) Mouse Untagged Clone

CAT#: MC221097

Brd4 (untagged) - Mouse bromodomain containing 4 (Brd4), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Brd4
• Synonyms: Brd5; HUNK1; MCAP; WI-11513
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221097 representing NM_198094
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTACGGAGAGCGGCCCTGGGACAAGATTGAGAAATCTGCCAGTAATGGGGGATGGACTAGAAACCT
  CCCAAATGTCTACAACGCAGGCCCAGGCCCAACCCCAGCCAGCAAATGCAGCCAGCACCAATCCTCCACC
  CCCAGAGACCTCCAACCCTAACAAGCCCAAGAGACAGACAAACCAACTGCAATATCTGCTCAGAGTGGTG
  CTCAAGACACTATGGAAACACCAGTTTGCGTGGCCTTTCCAGCAGCCCGTGGATGCCGTCAAGCTGAACC
  TCCCTGATTACTATAAGATTATTAAAACACCCATGGATATGGGAACAATAAAGAAGCGCTTGGAAAACAA
  CTATTACTGGAATGCTCAGGAATGTATCCAGGACTTCAACACTATGTTTACAAATTGTTACATCTATAAC
  AAGCCTGGAGATGACATCGTCTTAATGGCAGAAGCTCTGGAGAAGCTCTTCTTGCAAAAAATCAATGAAC
  TGCCTACAGAAGAAACTGAGATCATGATAGTCCAGGCAAAAGGAAGAGGACGAGGGAGGAAAGAAACAGG
  GGCAGCAAAGCCTGGTGTATCCACGGTACCAAACACAACTCAAGCATCAACTTCTCCGCAGACCCAGACG
  CCTCAGCAGAACCCTCCTCCACCTGTGCAGGCCACAACTCACCCCTTTCCTGCTGTCACCCCAGACCTCA
  TTGCCCAGCCTCCTGTCATGACAATGGTGCCCCCTCAGCCACTTCAGACTCCTTCACCGGTACCCCCCCA
  GCCACCACCCCCACCTGCTCCAGTTCCACAGCCTGTGCAGAGTCACCCGCCCATCATTGCGACCACCCCC
  CAGCCTGTGAAGACAAAGAAAGGGGTGAAGAGGAAAGCAGATACCACCACCCCTACCACCATCGACCCCA
  TTCATGAGCCACCCTCACTGGCCCCAGAGCCCAAGACCGCCAAGCTGGGTCCTCGGCGGGAGAGCAGCAG
  ACCTGTGAAGCCTCCAAAGAAGGATGTACCGGACTCACAGCAGCACCCAGGGCCAGAGAAGAGCAGCAAG
  ATCTCTGAGCAGCTAAAGTGCTGCAGTGGCATCCTCAAGGAGATGTTTGCCAAGAAACATGCTGCCTATG
  CCTGGCCTTTCTACAAGCCTGTGGATGTGGAGGCACTGGGTCTGCACGACTACTGTGACATCATCAAACA
  TCCCATGGACATGAGCACAATCAAGTCTAAACTAGAGTCCCGAGAGTACAGAGATGCCCAGGAATTTGGT
  GCTGATGTCCGATTGATGTTCTCCAACTGCTACAAGTACAACCCCCCTGACCATGAAGTGGTAGCCATGG
  CTCGAAAACTCCAGGATGTGTTTGAAATGCGCTTTGCCAAGATGCCTGATGAGCCTGAAGAGCCAGTTGT
  TACAGTGTCCTCTCCTGCAGTGCCACCCCCTACAAAGGTGGTAGCCCCACCCTCATCTAGTGACAGCAGC
  AGCGACAGTTCTTCCGACAGCGACAGTTCCACTGACGACTCTGAGGAAGAGCGAGCCCAGCGGCTGGCTG
  AACTCCAGGAACAGCTCAAGGCCGTGCATGAGCAGCTTGCAGCCCTCTCACAGCCCCAGCAGAACAAACC
  AAAGAAAAAGGAGAAGGACAAGAAGGAAAAGAAAAAGGAAAAGCACAAAAAGAAAGAAGAAGTGGAGGAA
  AATAAAAAAAGCAAAACCAAGGAACTTCCTCCCAAAAAGACAAAGAAAAATAACAGCAGCAACAGCAATG
  TGAGCAAGAAGGAACCAGTACCCACGAAGACCAAGCCGCCTCCCACATATGAATCAGAAGAGGAGGATAA
  GTGTAAGCCCATGTCTTATGAGGAGAAGCGGCAGCTAAGTCTAGATATCAACAAACTTCCTGGTGAGAAG
  CTAGGCCGTGTAGTACACATAATTCAGTCAAGGGAACCATCACTTAAAAACTCCAACCCCGATGAGATTG
  AGATTGACTTTGAGACCCTGAAGCCATCTACACTACGAGAGTTGGAGCGATATGTCACCTCCTGTTTGCG
  GAAGAAAAGGAAACCTCAAGCTGAAAAAGTTGACGTGATTGCTGGTTCTTCCAAGATGAAGGGATTCTCA
  TCCTCTGAGTCGGAGAGCACCAGCGAATCCAGCTCCTCTGACAGTGAAGACTCTGAAACAGGTCCTGCCT
  AA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_198094
• Insert Size: 2172 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198094.2, NP_932762.2
• RefSeq Size: 2731 bp
• RefSeq ORF: 2172 bp
• Locus ID: 57261
• UniProt ID: Q9ESU6
• Cytogenetics: 17 17.39 cM
• Gene Summary: This gene was temporarily named bromodomain-containing 5 (Brd5) and was renamed bromodomain-containing 4 (Brd4). [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) has two alternate splice sites, one in the 5' UTR and one in the 5' coding region, and also lacks several exons but includes an alternate 3' terminal exon, compared to variant 3. The resulting isoform (2) lacks an internal aa, and has a shorter and distinct C-terminus, compared to isoform 3.