Tap1 (NM_013683) Mouse Untagged Clone

CAT#: MC221090

Tap1 (untagged) - Mouse transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) (Tap1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tap1
• Synonyms: ABC17; Abcb; Abcb2; APT1; Ham; Ham-; Ham-1; Ham1; MTP; MTP1; PSF; PSF1; RI; RING4; T; TAP; Tap-1; Y3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221090 representing NM_013683
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGCACGTCTGGCTGGCGGCCGCCCTGCTCCTTCTGGTGGACTGGCTGCTGCTGCGGCCCATGC
  TCCCGGGAATCTTCTCCCTGTTGGTTCCCGAGGTGCCGCTGCTCCGGGTCTGGGTGGTGGGCCTGAGTCG
  CTGGGCCATCCTAGGACTAGGGGTCCGCGGGGTCCTCGGGGTCACCGCAGGAGCCCATGGCTGGCTGGCT
  GCTTTGCAGCCGCTGGTGGCCGCACTGAGTTTGGCCCTGCCTGGACTTGCCTTGTTCCGAGAGCTGGCCG
  CCTGGGGAACACTCCGGGAGGGTGACAGCGCTGGATTACTGTACTGGAACAGTCGTCCAGATGCCTTCGC
  TATCAGTTATGTGGCAGCATTGCCCGCAGCCGCCCTGTGGCACAAGTTGGGGAGCCTCTGGGCGCCCAGC
  GGCAACAGGGACGCTGGAGACATGCTGTGTCGGATGCTGGGCTTCCTGGGCCCTAAGAAGAGACGTCTCT
  ACCTGGTTCTGGTTCTCTTGATTCTCTCTTGCCTTGGGGAAATGGCCATTCCCTTCTTCACGGGCCGCAT
  CACTGACTGGATTCTTCAGGATAAGACAGTTCCTAGCTTCACCCGCAACATATGGCTCATGTCCATTCTC
  ACCATAGCCAGCACAGCGCTGGAGTTTGCAAGTGATGGAATCTACAACATCACCATGGGACACATGCACG
  GCCGTGTGCACAGAGAGGTGTTTCGGGCCGTCCTTCGCCAGGAGACAGGGTTTTTCCTGAAGAACCCAGC
  AGGTTCCATCACATCTCGGGTGACTGAGGACACAGCCAACGTGTGCGAGTCCATTAGTGGCACGCTGAGC
  CTGCTGCTGTGGTACCTGGGGCGAGCCCTGTGTCTCTTGGTGTTCATGTTTTGGGGGTCACCGTACCTCA
  CTCTGGTCACCCTGATCAACCTGCCCCTGCTTTTTCTTTTGCCTAAGAAGCTGGGAAAAGTGCATCAGTC
  ACTGGCAGTGAAGGTGCAGGAGTCTCTAGCAAAGTCCACGCAGGTGGCCCTTGAGGCCTTATCGGCGATG
  CCTACTGTGCGGAGCTTTGCCAACGAGGAGGGTGAGGCCCAGAAGTTCAGGCAGAAGTTGGAAGAAATGA
  AGACTCTAAACAAGAAGGAGGCCTTGGCTTACGTGGCTGAAGTCTGGACCACGAGTGTCTCGGGAATGCT
  GCTGAAGGTGGGAATTCTGTACCTGGGCGGGCAGCTGGTGATCAGAGGGGCTGTCAGCAGCGGCAACCTT
  GTCTCATTCGTTCTCTACCAGCTTCAGTTCACCCAGGCTGTTCAGGTCCTGCTCTCCCTCTACCCCTCCA
  TGCAGAAGGCTGTGGGCTCCTCAGAGAAAATATTCGAATACTTGGACCGGACTCCTTGCTCTCCACTCAG
  TGGCTCGTTGGCACCCTCAAACATGAAAGGCCTTGTGGAGTTCCAAGATGTCTCTTTTGCCTACCCAAAC
  CAGCCCAAAGTCCAGGTGCTTCAGGGGCTGACGTTCACCCTGCATCCTGGAACGGTGACAGCGTTGGTGG
  GACCCAATGGATCAGGGAAGAGCACCGTGGCTGCCCTGCTGCAGAACCTGTACCAGCCCACCGGGGGCCA
  GCTGCTGCTGGATGGCCAGTGCCTGGTCCAGTATGATCACCATTACCTGCACACTCAGGTGGCCGCAGTG
  GGACAAGAGCCGCTGCTATTTGGAAGAAGCTTTCGAGAAAATATTGCGTATGGCCTGAACCGGACTCCAA
  CCATGGAGGAAATCACAGCTGTGGCCGTGGAGTCTGGAGCCCACGATTTCATCTCTGGGTTCCCTCAGGG
  CTATGACACAGAGGTAGGTGAGACTGGGAACCAGCTGTCAGGAGGTCAGCGACAGGCAGTGGCCTTGGCC
  CGAGCCTTGATCCGGAAGCCACTCCTGCTTATCTTGGATGATGCCACCAGTGCCCTGGATGCTGGCAACC
  AGCTACGGGTCCAGCGGCTCCTGTATGAGAGCCCCAAGCGGGCTTCTCGGACGGTTCTTCTTATCACCCA
  GCAGCTCAGCCTGGCAGAGCAGGCCCACCACATCCTCTTTCTCAGAGAAGGCTCTGTCGGCGAGCAGGGC
  ACCCACCTGCAGCTCATGAAGAGAGGAGGGTGCTACCGGGCCATGGTAGAGGCTCTTGCGGCTCCTGCAG
  ACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013683
• Insert Size: 2175 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013683.2, NP_038711.2
• RefSeq Size: 2950 bp
• RefSeq ORF: 2175 bp
• Locus ID: 21354
• UniProt ID: P21958
• Cytogenetics: 17 17.98 cM
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This protein forms a heterodimer with Tap2 that transports short peptides from the cytosol into the endoplasmic reticulum lumen. Mutations in the human gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).