Stat3 (NM_011486) Mouse Untagged Clone

CAT#: MC221089

Stat3 (untagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Stat3
• Synonyms: 1110034C02Rik; A; Aprf; AW109958
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221089 representing NM_011486
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGTGGAACCAGCTGCAGCAGCTGGACACACGCTACCTGGAGCAGCTGCACCAGCTGTACAGCG
  ACAGCTTCCCCATGGAGCTGCGGCAGTTCCTGGCACCTTGGATTGAGAGTCAAGACTGGGCATATGCAGC
  CAGCAAAGAGTCACATGCCACGTTGGTGTTTCATAATCTCTTGGGTGAAATTGACCAGCAATATAGCCGA
  TTCCTGCAAGAGTCCAATGTCCTCTATCAGCACAACCTTCGAAGAATCAAGCAGTTTCTGCAGAGCAGGT
  ATCTTGAGAAGCCAATGGAAATTGCCCGGATCGTGGCCCGATGCCTGTGGGAAGAGTCTCGCCTCCTCCA
  GACGGCAGCCACGGCAGCCCAGCAAGGGGGCCAGGCCAACCACCCAACAGCCGCCGTAGTGACAGAGAAG
  CAGCAGATGTTGGAGCAGCATCTTCAGGATGTCCGGAAGCGAGTGCAGGATCTAGAACAGAAAATGAAGG
  TGGTGGAGAACCTCCAGGACGACTTTGATTTCAACTACAAAACCCTCAAGAGCCAAGGAGACATGCAGGA
  TCTGAATGGAAACAACCAGTCTGTGACCAGACAGAAGATGCAGCAGCTGGAACAGATGCTCACAGCCCTG
  GACCAGATGCGGAGAAGCATTGTGAGTGAGCTGGCGGGGCTCTTGTCAGCAATGGAGTACGTGCAGAAGA
  CACTGACTGATGAAGAGCTGGCTGACTGGAAGAGGCGGCAGCAGATCGCGTGCATCGGAGGCCCTCCCAA
  CATCTGCCTGGACCGTCTGGAAAACTGGATAACTTCATTAGCAGAATCTCAACTTCAGACCCGCCAACAA
  ATTAAGAAACTGGAGGAGCTGCAGCAGAAAGTGTCCTACAAGGGCGACCCTATCGTGCAGCACCGGCCCA
  TGCTGGAGGAGAGGATCGTGGAGCTGTTCAGAAACTTAATGAAGAGTGCCTTCGTGGTGGAGCGGCAGCC
  CTGCATGCCCATGCACCCGGACCGGCCCTTAGTCATCAAGACTGGTGTCCAGTTTACCACGAAAGTCAGG
  TTGCTGGTCAAATTTCCTGAGTTGAATTATCAGCTTAAAATTAAAGTGTGCATTGATAAAGACTCTGGGG
  ATGTTGCTGCCCTCAGAGGGTCTCGGAAATTTAACATTCTGGGCACGAACACAAAAGTGATGAACATGGA
  GGAGTCTAACAACGGCAGCCTGTCTGCAGAGTTCAAGCACCTGACCCTTAGGGAGCAGAGATGTGGGAAT
  GGAGGCCGTGCCAATTGTGATGCCTCCTTGATCGTGACTGAGGAGCTGCACCTGATCACCTTCGAGACTG
  AGGTGTACCACCAAGGCCTCAAGATTGACCTAGAGACCCACTCCTTGCCAGTTGTGGTGATCTCCAACAT
  CTGTCAGATGCCAAATGCTTGGGCATCAATCCTGTGGTATAACATGCTGACCAATAACCCCAAGAACGTG
  AACTTCTTCACTAAGCCGCCAATTGGAACCTGGGACCAAGTGGCCGAGGTGCTCAGCTGGCAGTTCTCGT
  CCACCACCAAGCGGGGGCTGAGCATCGAGCAGCTGACAACGCTGGCTGAGAAGCTCCTAGGGCCTGGTGT
  GAACTACTCAGGGTGTCAGATCACATGGGCTAAATTCTGCAAAGAAAACATGGCTGGCAAGGGCTTCTCC
  TTCTGGGTCTGGCTAGACAATATCATCGACCTTGTGAAAAAGTATATCTTGGCCCTTTGGAATGAAGGGT
  ACATCATGGGTTTCATCAGCAAGGAGCGGGAGCGGGCCATCCTAAGCACAAAGCCCCCGGGCACCTTCCT
  ACTGCGCTTCAGCGAGAGCAGCAAAGAAGGAGGGGTCACTTTCACTTGGGTGGAAAAGGACATCAGTGGC
  AAGACCCAGATCCAGTCTGTAGAGCCATACACCAAGCAGCAGCTGAACAACATGTCATTTGCTGAAATCA
  TCATGGGCTATAAGATCATGGATGCGACCAACATCCTGGTGTCTCCACTTGTCTACCTCTACCCCGACAT
  TCCCAAGGAGGAGGCATTTGGAAAGTACTGTAGGCCCGAGAGCCAGGAGCACCCCGAAGCCGACCCAGGT
  AGTGCTGCCCCGTACCTGAAGACCAAGTTCATCTGTGTGACACCATTCATTGATGCAGTTTGGAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011486
• Insert Size: 2169 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019168, AAH19168
• RefSeq Size: 2953 bp
• RefSeq ORF: 2169 bp
• Locus ID: 20848
• Cytogenetics: 11 63.82 cM
• Gene Summary: The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (3), also called Stat3B or Stat3beta, uses an alternate splice site in the 3' coding region, resulting in an early stop codon, compared to variant 1. The encoded isoform (3) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.