Pik3r1 (NM_001077495) Mouse Untagged Clone

CAT#: MC221084

Pik3r1 (untagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pik3r1
• Synonyms: p50alpha; p55alpha; p85alpha; PI3K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221084 representing NM_001077495
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGCAGAGGGCTACCAGTACAGAGCACTGTACGACTACAAGAAGGAGCGAGAGGAAGACATTGACC
  TACACCTGGGGGACATACTGACTGTGAATAAAGGCTCCTTAGTGGCACTTGGATTCAGTGATGGCCAGGA
  AGCCCGGCCTGAAGATATTGGCTGGTTAAATGGCTACAATGAAACCACTGGGGAGAGGGGAGACTTTCCA
  GGAACTTACGTTGAATACATTGGAAGGAAAAGAATTTCACCCCCTACTCCCAAGCCTCGGCCCCCTCGAC
  CGCTTCCTGTTGCTCCGGGTTCTTCAAAAACTGAAGCTGACACGGAGCAGCAAGCGTTGCCCCTTCCTGA
  CCTGGCCGAGCAGTTTGCCCCTCCTGATGTTGCCCCGCCTCTCCTTATAAAGCTCCTGGAAGCCATTGAG
  AAGAAAGGACTGGAATGTTCGACTCTATACAGAACACAAAGCTCCAGCAACCCTGCAGAATTACGACAGC
  TTCTTGATTGTGATGCCGCGTCAGTGGACTTGGAGATGATCGACGTACACGTCTTAGCAGATGCTTTCAA
  ACGCTATCTCGCCGACTTACCAAATCCTGTCATTCCTGTAGCTGTTTACAATGAGATGATGTCTTTAGCC
  CAAGAACTACAGAGCCCTGAAGACTGCATCCAGCTGTTGAAGAAGCTCATTAGATTGCCTAATATACCTC
  ATCAGTGTTGGCTTACGCTTCAGTATTTGCTCAAGCATTTTTTCAAGCTCTCTCAAGCCTCCAGCAAAAA
  CCTTTTGAATGCAAGAGTCCTCTCTGAGATTTTCAGCCCCGTGCTTTTCAGATTTCCAGCCGCCAGCTCT
  GATAATACTGAACACCTCATAAAAGCGATAGAGATTTTAATCTCAACGGAATGGAATGAGAGACAGCCAG
  CACCAGCACTGCCCCCCAAACCACCCAAGCCCACTACTGTAGCCAACAACAGCATGAACAACAATATGTC
  CTTGCAGGATGCTGAATGGTACTGGGGAGACATCTCAAGGGAAGAAGTGAATGAAAAACTCCGAGACACT
  GCTGATGGGACCTTTTTGGTACGAGACGCATCTACTAAAATGCACGGCGATTACACTCTTACACTAAGGA
  AAGGAGGAAATAACAAATTAATCAAAATCTTTCACCGTGATGGAAAATATGGCTTCTCTGATCCATTAAC
  CTTCAACTCTGTGGTTGAGTTAATAAACCACTACCGGAATGAGTCTTTAGCTCAGTACAACCCCAAGCTG
  GATGTGAAGTTGCTCTACCCAGTGTCCAAATACCAGCAGGATCAAGTTGTCAAAGAAGATAATATTGAAG
  CTGTAGGGAAAAAATTACATGAATATAATACTCAATTTCAAGAAAAAAGTCGGGAATATGATAGATTATA
  TGAGGAGTACACCCGTACTTCCCAGGAAATCCAAATGAAAAGAACGGCTATCGAAGCATTTAATGAAACC
  ATAAAAATATTTGAAGAACAATGCCAAACCCAGGAGCGGTACAGCAAAGAATACATAGAGAAGTTTAAAC
  GCGAAGGCAACGAGAAAGAAATTCAAAGGATTATGCATAACCATGATAAGCTGAAGTCGCGTATCAGTGA
  GATCATTGACAGTAGGAGGAGGTTGGAAGAAGACTTGAAGAAGCAGGCAGCTGAGTACCGAGAGATCGAC
  AAACGCATGAACAGTATTAAGCCGGACCTCATCCAGTTGAGAAAGACAAGAGACCAATACTTGATGTGGC
  TGACGCAGAAAGGTGTGCGGCAGAAGAAGCTGAACGAGTGGCTGGGGAATGAAAATACCGAAGATCAATA
  CTCCCTGGTAGAAGATGATGAGGATTTGCCCCACCATGACGAGAAGACGTGGAATGTCGGGAGCAGCAAC
  CGAAACAAAGCGGAGAACCTATTGCGAGGGAAGCGAGACGGCACTTTCCTTGTCCGGGAGAGCAGTAAGC
  AGGGCTGCTATGCCTGCTCCGTAGTGGTAGACGGCGAAGTCAAGCATTGCGTCATTAACAAGACTGCCAC
  CGGCTATGGCTTTGCCGAGCCCTACAACCTGTACAGCTCCCTGAAGGAGCTGGTGCTACATTATCAACAC
  ACCTCCCTCGTGCAGCACAATGACTCCCTCAATGTCACACTAGCATACCCAGTATATGCACAACAGAGGC
  GATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001077495
• Insert Size: 2175 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077495.2, NP_001070963.1
• RefSeq Size: 6928 bp
• RefSeq ORF: 2175 bp
• Locus ID: 18708
• UniProt ID: P26450
• Cytogenetics: 13 53.92 cM
• Gene Summary: Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (By similarity). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348926).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2).