Kcnq2 (NM_001006669) Mouse Untagged Clone

CAT#: MC221079

Kcnq2 (untagged) - Mouse potassium voltage-gated channel, subfamily Q, member 2 (Kcnq2), transcript variant 5, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnq2
• Synonyms: HNSPC; KQT2; Nmf134
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221079 representing NM_001006669
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGAAGTCGCGCAACGGTGGCGTGTACCCCGGCACCAGCGGGGAAAAGAAGCTCAAGGTGGGCT
  TCGTGGGGCTGGACCCCGGCGCGCCCGACTCCACACGCGACGGCGCGCTACTCATCGCGGGCTCCGAGGC
  CCCCAAGCGCGGCAGCGTTTTGAGCAAGCCGCGGACGGGCGGCGCGGGAGCCGGGAAGCCCCCGAAGCGC
  AACGCCTTCTACCGCAAGCTGCAGAATTTCCTCTACAACGTGCTAGAGCGGCCCCGCGGCTGGGCGTTCA
  TCTACCACGCCTACGTGTTCCTTTTAGTCTTCTCCTGCCTTGTGCTTTCTGTGTTTTCCACCATCAAGGA
  GTACGAGAAGAGCTCTGAGGGGGCCCTCTACATCTTGGAAATCGTGACTATCGTGGTATTCGGTGTTGAG
  TACTTTGTGAGGATCTGGGCTGCAGGCTGCTGTTGCCGGTATCGAGGCTGGAGGGGCAGGCTCAAGTTTG
  CCAGGAAGCCGTTCTGTGTGATTGATATCATGGTGCTGATTGCCTCCATTGCTGTGCTGGCTGCTGGTTC
  CCAGGGCAATGTCTTTGCCACATCTGCGCTTCGGAGCTTGCGGTTCTTGCAAATCTTGCGGATGATCCGT
  ATGGACCGGAGGGGTGGCACCTGGAAGCTCTTGGGATCGGTAGTCTACGCTCACAGCAAGGAGCTGGTGA
  CTGCCTGGTACATTGGCTTCCTCTGCCTCATCCTGGCCTCATTTCTGGTGTACTTGGCAGAAAAGGGTGA
  GAATGACCACTTTGACACCTACGCAGATGCACTCTGGTGGGGTCTGATCACCCTGACGACCATTGGCTAC
  GGGGACAAGTACCCTCAGACCTGGAACGGGAGGCTGCTGGCAGCGACCTTTACCCTCATTGGTGTCTCGT
  TCTTTGCTCTTCCTGCTGGCATTTTGGGATCCGGCTTTGCCCTGAAAGTCCAAGAGCAGCATCGGCAAAA
  ACACTTTGAGAAACGGCGGAACCCTGCGGCAGGTCTGATCCAGTCTGCCTGGAGATTCTATGCTACTAAC
  CTCTCACGCACCGACCTGCACTCCACGTGGCAGTACTACGAGCGGACAGTCACTGTCCCCATGTACAGAC
  TCATCCCACCTCTGAACCAGCTGGAGCTGCTGAGGAATCTCAAGAGCAAATCTGGACTCACCTTCAGGAA
  GGAGCCACAGCCAGAGCCATCACCAAGTCAGAAGGTCAGTTTGAAAGATCGTGTCTTCTCCAGCCCCCGA
  GGCATGGCTGCCAAGGGAAAGGGGTCTCCCCAGGCCCAGACGGTCCGGCGGTCCCCCAGTGCGGATCAGA
  GTCTTGATGACAGCCCGAGCAAGGTGCCCAAGAGCTGGAGCTTTGGTGACCGCAGCCGCACACGCCAGGC
  TTTCCGCATCAAGGGTGCTGCATCCCGGCAGAATTCAGAAGAAGCAAGCCTCCCTGGGGAGGACATCGTA
  GAGGACAACAAGAGCTGTAACTGCGAGTTTGTGACTGAAGATCTTACCCCTGGCCTCAAAGTTAGCATCA
  GAGCTGTGTGTGTTATGCGGTTCTTGGTATCTAAGCGAAAGTTCAAAGAGAGTCTGCGCCCATATGATGT
  GATGGACGTCATCGAACAGTACTCGGCTGGACACTTGGATATGTTGTCCCGCATCAAGAGCCTGCAGTCC
  AGAGTGGACCAGATTGTGGGGCGGGGCCCAACAATAACGGATAAGGACCGCACCAAAGGCCCAGCGGAAA
  CGGAGCTGCCCGAAGACCCCAGCATGATGGGACGGCTTGGGAAGGTGGAGAAACAGGTCTTGTCCATGGA
  AAAGAAGCTCGACTTCTTGGTGAGCATCTATACACAGAGAATGGGCATCCCACCAGCAGAGACAGAGGCC
  TATTTTGGGGCCAAGGAGCCTGAGCCGGCACCACCCTACCACAGCCCAGAGGACAGCCGTGACCATGCAG
  ACAAGCATGGCTGTATCATTAAGATCGTCCGCTCCACCAGCTCTACGGGCCAGAGGAACTACGCAGCACC
  CCCAGCCATCCCCCCTGCCCAGTGTCCTCCCTCCACCTCGTGGCAGCAGAGCCACCAGCGCCATGGCACC
  TCCCCTGTGGGAGACCATGGCTCACTGGTCCTGCGACTGGAGAGGAGTGCTGGCATGATGAGCTGTCACT
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001006669
• Insert Size: 2172 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001006669.2, NP_001006670.1
• RefSeq Size: 2965 bp
• RefSeq ORF: 2172 bp
• Locus ID: 16536
• Cytogenetics: 2 103.57 cM
• Gene Summary: Associates with KCNQ3 to form a potassium channel with essentially identical properties to the channel underlying the native M-current, a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs. Therefore, it is important in the regulation of neuronal excitability.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) lacks two alternate in-frame exons, and it also contains alternate 3' exon structure resulting in an alternate 3' coding region, compared to variant 1. The encoded isoform (5) has a distinct C-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.