Ddx4 (NM_001145885) Mouse Untagged Clone

CAT#: MC221072

Ddx4 (untagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (Ddx4), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ddx4
• Synonyms: AV206478; Mvh; VASA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221072 representing NM_001145885
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGATGAAGATTGGGAGGCAGAAATACTCAAACCTCACGTGTCTTCCTATGTTCCTGTATTTGAGA
  AGGATAAATATTCTTCTGGAGCAAATGGAGACACTTTTAACAGGACTTCAGCTTCATCAGAAATGGAAGA
  TGGGCCTTCTGGAAGAGATGATTTCATGAGAAGTGGGTTTCCTTCTGGAAGAAGTTTAGGAAGCAGAGAT
  ATTGGCGAGTCTAGTAAAAAAGAGAACACATCTACAACTGGTGGCTTTGGAAGAGGAAAGGGCTTTGGAA
  ACAGAGGTTTTTTAAATAACAAGTTTGAAGAAGGTGATAGCTCTGGTTTCTGGAAAGAGTCTAATAATGA
  CTGTGAAGATAATCAGACTCGAAGCAGAGGGTTTTCCAAGCGAGGTGGCTGCCAAGATGGAAACGATTCA
  GAAGCATCAGGCCCGTTCAGAAGAGGGGGAAGAGGCAGTTTCCGAGGCTGCCGTGGAGGATTTGGTCTAG
  GAAGACCAAATAGTGAATCTGACCAAGATCAGGGGACACAGCGTGGTGGTGGCCTTTTTGGTTCTAGGAA
  ACCAGCAGCAAGTGATTCAGGCAATGGTGACACTTACCAAAGCAGAAGTGGAAGTGGTCGAGGTGGTTAC
  AAAGGTTTAAATGAAGAAGTAGTAACAGGTTCTGGAAAGAATTCTTGGAAGTCAGAAACTGAAGGAGGTG
  AAAGCAGTGATAGTCAAGGTCCAAAAGTGACATATATACCCCCTCCTCCACCAGAGGATGAGGACTCCAT
  CTTTGCACATTATCAGACAGGCATAAACTTTGATAAATATGACACCATACTTGTTGAAGTATCTGGACAT
  GATGCACCACCGGCAATTTTGACTTTTGAAGAAGCTAATCTCTGTCAGACACTGAATAACAACATTGCTA
  AAGCTGGCTATACTAAGCTTACTCCTGTGCAGAAGTACAGCATTCCCATTGTATTAGCAGGACGAGATTT
  GATGGCTTGTGCTCAAACAGGGTCTGGGAAGACTGCAGCTTTTCTCTTGCCTATTTTGGCTCATATGATG
  CGGGATGGAATAACTGCCAGTCGCTTTAAAGAACTGCAGGAACCAGAGTGTATTATTGTAGCACCAACTC
  GAGAACTGATCAACCAAATTTACTTGGAAGCCAGAAAATTTTCTTTTGGGACTTGTGTAAGAGCTGTGGT
  CATATATGGAGGAACCCAGTTTGGTCATTCAGTTCGACAGATAGTACAAGGGTGTAACATATTGTGTGCT
  ACTCCAGGGAGGCTGATGGACATCATAGGCAAAGAAAAGATTGGCCTCAAACAAGTCAAGTACTTAGTTT
  TGGATGAAGCTGATCGAATGTTGGATATGGGTTTTGGACCAGAGATGAAGAAACTAATTTCTTGTCCAGG
  AATGCCATCAAAGGAACAACGCCAAACCCTTTTATTCAGTGCTACTTTTCCAGAAGAAATCCAGAGGTTG
  GCTGGGGACTTTCTAAAGTCCAGTTACTTGTTTGTCGCTGTTGGGCAAGTGGGAGGAGCTTGCAGAGATG
  TTCAGCAGACGATCCTTCAAGTTGGCCAGTATTCAAAAAGAGAAAAGCTTGTTGAGATTCTACGAAACAT
  AGGTGATGAAAGAACTATGGTCTTTGTTGAAACCAAGAAAAAAGCCGATTTCATTGCAACTTTTCTTTGT
  CAAGAAAAAATATCAACTACAAGTATTCATGGTGATCGGGAGCAGAGGGAGAGAGAGCAAGCTCTTGGAG
  ATTTTCGCTGTGGAAAGTGCCCAGTTCTTGTTGCTACTTCAGTGGCTGCCAGAGGGCTTGATATTGAAAA
  TGTTCAACATGTTATCAATTTTGACCTTCCTTCTACCATTGATGAGTATGTTCATCGAATTGGACGCACT
  GGCCGCTGTGGAAATACTGGCAGAGCGATTTCTTTTTTTGATACTGACTCTGATAATCATTTAGCACAGC
  CTCTAGTTAAAGTACTGTCAGACGCTCAACAGGATGTCCCCGCATGGCTAGAAGAGATTGCCTTCAGTAC
  CTATGTGCCTCCCAGCTTCAGTAGCAGCACAAGAGGGGGGGCTGTGTTTGCATCTGTTGACACGAGGAAG
  AATTACCAGGGCAAGCACACGTTGAATACAGCGGGGATTTCTTCTTCACAAGCTCCCAATCCAGTTGATG
  ACGAGTCATGGGATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001145885
• Insert Size: 2187 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145885.1, NP_001139357.1
• RefSeq Size: 2850 bp
• RefSeq ORF: 2187 bp
• Locus ID: 13206
• Cytogenetics: 13 63.87 cM
• Gene Summary: ATP-dependent RNA helicase required during spermatogenesis to repress transposable elements and preventing their mobilization, which is essential for the germline integrity (PubMed:20439430, PubMed:28633017). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:20439430, PubMed:28633017). Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (PubMed:20439430, PubMed:28633017). Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs (PubMed:28633017).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1).