Ccnt1 (NM_009833) Mouse Untagged Clone

CAT#: MC221070

Ccnt1 (untagged) - Mouse cyclin T1 (Ccnt1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ccnt1
• Synonyms: 2810478G24Rik; AI115585; CycT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221070 representing NM_009833
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGAGAGAGGAAGAACAACAACAAGCGGTGGTATTTTACTCGCGAGCAGCTGGAGAACAGCCCGT
  CGCGTCGCTTTGGCGTGGACTCCGATAAAGAACTCTCTTACCGCCAGCAGGCGGCCAATCTCCTCCAGGA
  CATGGGACAACGTCTGAACGTCTCACAACTGACGATCAATACTGCTATAGTATACATGCACCGATTCTAC
  ATGATTCAGTCTTTTACACAGTTTCATCGATATTCCATGGCTCCAGCAGCCTTGTTTCTAGCAGCAAAAG
  TAGAAGAACAACCAAAGAAATTGGAGCATGTCATCAAGGTGGCACACACTTGCCTGCACCCACAGGAGTC
  ACTTCCTGACACGAGGAGTGAGGCTTACCTACAACAAGTTCAAGATCTGGTGATTTTAGAGAGTATAATT
  TTGCAGACCTTAGGGTTTGAACTAACAATTGATCACCCACATACACATGTGGTAAAGTGCACCCAGCTTG
  TTCGAGCAAGCAAGGACTTAGCACAGACATCTTACTTCATGGCAACCAACAGCCTGCATTTGACCACATT
  TAGCCTGCAGTACACACCTCCTGTGGTGGCCTGTGTCTGCATCCATCTGGCTTGCAAGTGGTCCAACTGG
  GAGATCCCAGTCTCAACTGACGGGAAGCACTGGTGGGAGTATGTTGACGCCACTGTGACCTTGGAACTGT
  TAGATGAATTGACACATGAATTTCTACAGATTCTAGAGAAAACTCCAAGCAGGCTGAAACGTATTCGGAA
  TTGGAGGGCGTACCAGGCTGCCATGAAAACCAAGCCAGATGACCGAGGAGCAGATGAAAACACGTCGGAG
  CAGACAATCCTCAATATGATTTCTCAGACCTCTTCAGACACAACTATCGCAGGATTAATGAGCATGTCAA
  CTGCGTCTACAAGTGCAGTGCCTTCCCTGCCTTCCTCTGAGGAGTCATCGAGCAGTCTAACTAGTGTGGA
  TATGTTGCAGGGCGAGCGGTGGCTGTCTTCCCAACCTCCGTTTAAACTCGAAGCTGCCCAGGGTCATCGG
  ACTAGTGAGAGTTTAGCACTTATTGGCGTTGATCATTCCTTGCAACAGGATGGTTCCAGTGCGTTTGGTT
  CCCAGAAGCAGGCGAGCAAGAGTGTGCCATCTGCTAAGGTTTCACTTAAGGAGTACCGTGCAAAGCACGC
  GGAAGAGTTGGCTGCTCAGAAGAGGCAGCTGGAGAACATGGAGGCCAATGTGAAGTCACAGTATGCGTAT
  GCTGCCCAGAATCTCCTGTCTCACGACAGCCATTCTTCAGTTATTCTCAAAATGCCCATTGAGAGCTCAG
  AAAACCCTGAACGTCCTTTTCTGGATAAGGCTGACAAATCAGCTCTGAAAATGAGACTCCCTGTGGCCAG
  TGGGGATAAAGCAGTTTCTTCCAAGCCAGAGGAGATAAAGATGCGCATTAAAGTTCACTCTGCAGGGGAT
  AAGCACAATTCCATAGAAGACAGTGTCACCAAGAGCCGCGAGCACAAGGAGAAGCAGAGGACTCACCCGT
  CTAACCACCACCACCATCACAACCACCACTCACACAGGCACTCTCACTTACAGCTGCCAGCCGGCCCCGT
  GAGCAAACGCCCAAGTGATCCAAAACACAGCAGCCAGACAAGCACCTTAGCACACAAAACCTACAGCTTG
  TCCAGTACTTTGTCTTCTTCCAGCTCTACTCGTAAAAGGGGTCCCCCGGAAGAGACTGGGGCAGCAGTAT
  TTGATCATCCAGCCAAGATTGCCAAGAGTACTAAATCTTCCTTGAATTTCCCCTTCCCCCCCCTTCCTAC
  AATGACCCAGTTGCCTGGGCATAGCTCAGACACAAGTGGCCTGCCCTTTTCACAGCCTAGCTGTAAAACT
  CGAGTTCCTCATATGAAACTGGATAAAGGCCCTCCTGGGGCTAATGGTCACAACGCAACTCAGTCAATAG
  ATTATCAAGACACTGTGAATATGCTCCACTCCCTGCTTAGTGCCCAGGGGGTTCAGCCCACACAGGCCCC
  TGCATTTGAATTTGTCCACTCTTATGGTGAATATATGAATCCCAGAGCTGGTGCCATCTCCTCCAGATCT
  GGCACTACAGACAAACCTCGGCCACCACCCCTCCCATCAGAACCCCCTCCACCACTTCCACCTCTTCCTA
  AGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009833
• Insert Size: 2175 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009833.1, NP_033963.1
• RefSeq Size: 2175 bp
• RefSeq ORF: 2175 bp
• Locus ID: 12455
• UniProt ID: Q9QWV9
• Cytogenetics: 15 F1
• Gene Summary: Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.