Agbl5 (NM_174849) Mouse Untagged Clone

CAT#: MC221045

Agbl5 (untagged) - Mouse ATP/GTP binding protein-like 5 (Agbl5), transcript variant 5, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Agbl5
• Synonyms: 9430057O19Rik; CCP5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221045 representing NM_174849
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGCGCTGTGGGGGATTGCTGTTCAGTTCTCGCTTTGATTCAGGGAACCTAGCCCATGTGGAAA
  AGGTGGAGACTGTGTCTAGTGATGGGGAAGGCGTAGGAGGGGTGGCAACAGCCCCCGCTAGTGGGTCTGC
  TGCTTCCCCTGACTACGAGTTCAACGTGTGGACCCGGCCTGACTGTGCTGAAACGGAATATGAAAATGGC
  AACAGGTCATGGTTCTACTTCAGTGTCCGGGGAGGGACTCCAGGAAAGCTTATCAAGATCAACATTATGA
  ACATGAACAAGCAAAGCAAACTGTATTCCCAGGGCATGGCCCCCTTTGTGCGCACACTGCCTTCCCGGCC
  ACGCTGGGAACGCATTAGAGAGCGGCCCACCTTTGAGATGACAGAGACACAGTTTGTGCTATCCTTTGTT
  CACCGTTTCGTAGAGGGCCGGGGAGCCACCACCTTCTTCGCCTTCTGCTACCCCTTCTCCTACAGTGACT
  GCCAGGATCTGCTGAGCCAGCTAGACCAGCGCTTTTCAGAGAATTACTCTACCCATAGCAGTCCTCTGGA
  TAGTATTTATTACCACCGAGAGCTTCTCTGCTATTCTCTGGATGGACTTCGTGTAGATCTACTAACAATC
  ACTTCCTGCCATGGGCTTCGAGATGATCGGGAGCCCCGACTAGAGCAGCTATTTCCTGATCTCGGCACTC
  CCCGACCATTCCGTTTCACAGGCAAAAGGATATTCTTCTTAAGCAGTAGGGTACACCCTGGAGAGACTCC
  ATCTAGCTTTGTGTTCAATGGCTTTCTGGACTTCATCCTTCGACCTGATGATCCCCGGGCACAAACTCTC
  CGTCGACTCTTTGTTTTTAAGTTGATTCCCATGTTAAACCCTGATGGTGTGGTCCGGGGCCACTACCGCA
  CGGACTCCCGTGGAGTGAATCTGAACCGTCAGTACTTGAAGCCTGATGCCGTCCTGCACCCGGCCATCTA
  TGGCGCCAAAGCTGTGCTGCTTTATCATCACGTGCACTCGAGGCTCAACGCCAAGAGTCCCACGAACCAG
  CAACCCACTCTCCATCTCCCTCCGGAAGCCCCTCTCTCTGACCTCGAGAAAGCCAATAATCTCCACAATG
  AAGCTCACCTCGGGCAGTCACCTGATGGGGAAAACCCTGCGACTTGGCCTGAGACAGAGCCAGCAGAAGA
  GAAGACTGACCCTGTGTGGCTTATGCCACAGCCAATTCCTGAACTCGAGGAGCCAGCCCCTGACACCATC
  CCCCCAAAAGAGAGTGGTGTTGCTTACTATGTAGACTTGCACGGACATGCTTCCAAAAGGGGCTGCTTCA
  TGTATGGCAACAGCTTTAGTGACGAGAGCACCCAGGTGGAAAACATGCTATATCCAAAGCTCATCTCCTT
  GAATTCAGCCCACTTTGACTTCCAGGGCTGCAATTTCTCAGAGAAGAACATGTATGCCCGAGACCGTAGA
  GATGGCCAGTCCAAAGAGGGAAGCGGCCGTGTCGCAATCTACAAAGCCTCAGGGATAATCCACAGCTACA
  CACTTGAATGCAACTACAACACTGGACGCTCAGTCAACAGCATCCCTGCTGCCTGCCATGACAACGGGCG
  CGCCAGCCCCCCTCCCCCTCCGGCTTTCCCCTCCAGATACACTGTGGAACTATTCGAGCAGGTGGGGCGA
  GCTATGGCCATCGCAGCTCTGGACATGGCAGAGTGTAATCCGTGGCCCCGAATTGTGCTGTCGGAACACA
  GCAGCCTCACCAACCTGCGAGCCTGGATGCTGAGGCACGTGCGCAACAGCAGAGGGCTGACCAGTGCTGG
  GAACATGGGCGCGAGCAAGAAGCGAGGGGCTCGAACCCCTCCCAAAAGCAACAACAGCTTGCCTGTTTCC
  TGCTCTGAAAATGCTCTGAGCCGGGTTCGCAGTTTTAGCACCGGCACGAGTACTGGTGGCAGCAGCAGCA
  GCCAACAAAACTCTCCACAGATGAAGAACTCTCCCAGCTTCCCTTTTCATGGCAGTCGGACTGCGGGGCT
  ACCAGGCCTGGGCTCTAGCACCCAAAAGGTCAGCCATCGGGTGCTGGGCCCTGTCAGAGGTAAGCCAGTC
  TGGGAGCCCCTGCAGCAGGTGTTTGGTTGCTTGGGGCATTGCTGGGGAGAAAGAGCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_174849
• Insert Size: 2160 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_174849.3, NP_777274.1
• RefSeq Size: 2922 bp
• RefSeq ORF: 2160 bp
• Locus ID: 231093
• UniProt ID: Q09M02
• Cytogenetics: 5 B1
• Gene Summary: Metallocarboxypeptidase that mediates protein deglutamylation. Specifically catalyzes the deglutamylation of the branching point glutamate side chains generated by post-translational glutamylation in proteins such as tubulins (PubMed:20519502, PubMed:21074048). In contrast, it is not able to act as a long-chain deglutamylase that shortens long polyglutamate chains, a process catalyzed by AGTPBP1/CCP1, AGBL2/CCP2, AGBL3/CCP3, AGBL1/CCP4 and AGBL4/CCP6 (PubMed:25103237). Mediates deglutamylation of CGAS, regulating the antiviral activity of CGAS (PubMed:26829768).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) has multiple differences, compared to variant 3. The resulting protein (isoform 5), has a shorter and distinct C-terminus when it is compared to isoform 3.