Rtf1 (NM_030112) Mouse Untagged Clone

CAT#: MC221020

Rtf1 (untagged) - Mouse Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (Rtf1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rtf1
• Synonyms: 2900005O08Rik; 6530416A09Rik; AI853581; AU043144; AW553985; cbp82; Gtl7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221020 representing NM_030112
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGGTCGCCTTTGTGTGGGTCGAGCGGCGGCGGTGGCGGCGGCGGTGGCGGCGGCGGCAGTGGCGG
  TCCCACTGGCAGGCGGGCAAGAGGGGAGTCAGGGCGGCGTCCGGCGTGGAAGCCGGGGGACCACCATGGT
  AAAGAAGCGGAAAGGCCGTGTCGTGATCGACTCGGATACGGAAGACAGCGGCAGTGACGAGAACCTGGAT
  CAGGAGCTCTTGTCCCTGGCGAAACGGAAGCGCAGTGACTCCGAGGAGAAGGAGCCTCCTGTGAGTCAGC
  CTGCAGCCTCATCAGATTCAGAGACCTCCGACAGTGATGATGAGTGGACATTTGGGAGCAATAAAAATAA
  GAAGAAAGGAAAAACCAGAAAAGTAGAGAAGAAAGGAGCCATGAAGAAGCAAGCCAACAAAGCTGCCTCC
  TCGGGCAGCTCAGACAGAGACAGCTCAGCTGAGAGCTCTGCCCCTGAGGAAGGTGAAGTGTCAGATTCTG
  AGAGCAGCAGCTCCTCTTCCAGTTCAGATTCAGACTCCTCGTCAGAAGATGAAGAATTCCATGATGGCTA
  TGGAGAGGATCTTATGGGAGATGAGGAAGATAGGGCCCGACTGGAGCAGATGACGGAGAAGGAGAGGGAG
  CAGGAGCTCTTCAACCGCATAGAGAAGAGAGAGGTGTTGAAAAGAAGATTTGAAATTAAGAAAAAACTAA
  AAACAGCCAAAAAGAAAGAGAAGAAAGAAAAAAAGAAAAAGCAGGAAGAAGAACAAGAAAAGAAGAAGCT
  GACACAGATTCAGGAATCTCAGGTTACTTCCCACAATAAAGAACGGCGTTCCAAGCGGGATGAAAAACTA
  GATAAGAAATCTCAAGCCATGGAGGAGCTAAAAGCAGAGCGAGAAAAACGGAAGAACAGAACAGCTGAGC
  TCCTCGCCAAAAAACAGCCATTGAAAACCAGTGAGGTCTACTCTGACGATGAGGAGGAGGAAGATGATGA
  CAAGTCCAGTGAGAAATCGGACCGCTCATCCCGGACATCATCATCTGATGAAGAGGAAGAGAAAGAAGAA
  ATCCCTCCAAAATCACAGCCAGTCTCATTACCCGAGGAACTGAATCGGGTTCGGTTATCACGGCATAAGC
  TAGAGCGCTGGTGTCACATGCCCTTCTTTGCTAAAACTGTCACAGGATGTTTTGTACGGATTGGCATTGG
  AAACCACAATAGCAAGCCAGTTTACCGGGTGGCTGAGATCACGGGTGTTGTGGAAACTGCCAAAGTTTAC
  CAGCTGGGTGGCACCAGAACAAATAAAGGGCTCCAGCTACGGCATGGCAATGACCAGCGAGTGTTCCGCC
  TAGAGTTTGTGTCCAACCAAGAATTCACTGAAAGTGAATTCATGAAGTGGAAAGAAGCGATGTTTTCTGC
  TGGCATGCAGTTGCCCACACTAGATGAAATCAATAAAAAGGAATTATCTATTAAGGAAGCTCTCAATTAT
  AAATTCAATGATCAGGACATTGAGGAGATTGTAAAAGAGAAGGAAAGGTTCAGAAAAGCCCCACCAAACT
  ATGCTATGAAGAAGACTCAGCTGCTAAAGGAGAAGGCTATGGCTGAGGACCTGGGAGATCAGGACAAAGC
  CAAACAAATCCAAGACCAGCTAAATGAGCTAGAGGAGCGAGCAGAGGCCCTGGACCGCCAGCGGACCAAG
  AACATATCTGCTATCAGCTACATCAACCAGCGGAATCGGGAGTGGAATATTGTGGAGTCTGAGAAGGCTC
  TGGTGGCTGAAAGTCACAACATGAGAAACCAACAGATGGACCCCTTCACCAGGCGGCAGTGCAAACCCAC
  CATCGTCTCTAATTCCAGGGATCCAGCTGTTCAGGCTGCCATTTTGGCCCAGCTCAATGCAAAATACGGT
  TCTGGGGTATTACCAGATGCTCCAAAGGAAATGAGCAAGGGTCAAGGAAAGGATAAAGATTTGAATTCTA
  AGACAGCCAGTGACCTCTCGGAAGACCTGTTCAAAGTGCATGACTTTGACGTGAAGATTGACTTACAGGT
  TCCAAGCTCAGAGTCAAAAGCTTTGGCCATTACCTCCAAAGCCCCACCAGCCAAGGACGGAGCTCCGAGG
  AGATCTCTGAACTTAGAAGACTACAAAAAACGACGGGGGCTTATTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030112
• Insert Size: 2148 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030112.2, NP_084388.2
• RefSeq Size: 4680 bp
• RefSeq ORF: 2148 bp
• Locus ID: 76246
• UniProt ID: A2AQ19
• Cytogenetics: 2 E5
• Gene Summary: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Binds single-stranded DNA (By similarity). Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex.[UniProtKB/Swiss-Prot Function]