Bbs10 (NM_027914) Mouse Untagged Clone

CAT#: MC221015

Bbs10 (untagged) - Mouse Bardet-Biedl syndrome 10 (human) (Bbs10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bbs10
• Synonyms: 1300007O09Rik; AI452285
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221015 representing NM_027914
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCCAGGGGTCGGTGACGGCGGCTTTGCGGGTGGCCGAGGTGCTGGAGAGCATCGCGAACCGCT
  GCGTGGGGCCCGAGGGCGGCCAAGTGTTGTGTACGAAGCCCACCGGCGAGGTGCTGCTTAGCAGGGATGG
  AGGCTGCCTCCTGGAGGCGCTGCACTTAGAGCATCCCTTAGCCAGGATGATAGTGGCGTGTGTTTCCAGT
  CACCTTAAAAAGACAGGAGATGGTGCTAAGACATTTATTATTTTTCTTTGCCATTTACTCAGAGGACTTC
  ATGCAATCGGAGAAAAAGGAAAAGATTCATTCACTTCTGAAAATATTCAGAGCCATGAAAGGCATTGGAA
  AAACTGTTGTCAGTGGAAATCTATTTCCCAGGCTCTGCAAACGTTTCAGACACAAACCCTAGGTTGTATT
  GTAGACCGGAGTTTAAGCAGGCACTACCTGTCTGTCTTTTCTTCATCCACTGAAGGAAGAAAACTGTGCA
  GGCACTCGCTAGAGTTGCTCCTGGAAGCATACTTTTGTGGAAGAGTGGGAAGAAACAATCACAGATTCAT
  TTCACAGTTGATGTGTGACTACGTTTTCAAGTGTATGGCTTGTGAAAGTGGGGTTGAAGTATTTGAGTTG
  TTGGATCACTGTTTTGCAGAGTTGAATGTTGGCGTGACAGGCCTTCCTGTTTCAGATTCTAGAATCATAG
  ATGGCCTTGTGCTTCCCAGGGACTTTTCTATGTACTGCCCAGCAGATGGTGACATTAGGATGGTGATAGT
  AACAGAAATCCTCCAGCCTCAGTTTTCATCGGCTGGCTCGGAGTTTGTTCTCAATTCAGAAACACAGTTT
  CAGGCATCTCAGTGCTGGATCACGGACAGGACAAAAACAGTAATGAACCACTTGCGTGGTCAGAATGTAA
  AACTGCTCCTGACCAGTGTGAAGCAACCAGACCTGGTTATTTACTGTGCAAGACTTAATAGCATATCAGT
  GGTGGAGTGTTTATCAGCCGAAGAAGTTTCCCTTGTCCAGAGGATCACTGGTCTTTCTCCTTGTGTCCTA
  CCAGAGGTTGCTTCACAGTGTGAAATTTCTGATAGTACTTTGGTGAAGTTTTGCAAGCCCCTCATCCTTA
  GATCCAAGAGGTATGTTCATCTCGGCTTGATTAGCACATGTGCATTTATACCTCACTCTATGGTCCTCTG
  TGGACCGGTTCTGGGTCTTGTTGAACAGCATGAGAGAGCTTTTCACGGAGCATTTAAGATGCTTCGGCAG
  CTATTTACTGACCTTGATCTAAATTACATCATACAAACCAAACAGCAATGTAACCCAAGTCCTCTTGCTT
  ATGACAATAGCAGAGAGCGTAATCACTCACCAGAAACTGATAAGTATCAGGACATAGTAGCAAAGAGCAA
  AAATAAATTGGAAACTCAAACACATTTAGAAGTATATTCAGGTTTGGGGGCTTCAGATACAGAATTGAGA
  GCAGGTAAGCCGTGGTCAGCACACAAGAAGACACCAATAGCTCCATCCCAAACAGATGAAATGCTGAAGT
  GCTTGCCTCCAGAAAGAAGCGGGATAATTGATAACTGTGACCTGTCAATTGAGAATCATTCCACTGGAAA
  TCCTACAGCAGAAGACACTGGAACAGAAATTTCTTTCGAACACTTACAGGTCTCAGATAATGCCGGAAAG
  GGTTACACGCTGCCAGTGATGCGCAAGTCACTAGATACCTGTACTTGTCAGGGTTATTGTTCATCCACTG
  TGCCAGCAGGCTGTGTGCTGCCAGTGGGTGGAAGTTTTGAGATTTTGATGAGTTACTACCTTCTCAGCTA
  TGCCAAACAATGCCGACAATCAGATGAAACTGTCATCAGTATGTTAATAGCTGATGCTCTTCTAGGCATC
  CCTAAAATCCTTTATAAGCCTAAGAAGGGAAAGGACAGTTTCCCACACATATACATGCGGTCTCTCCATG
  CACTGCAAGCCAGTCAACCCATGGTAAGTGGTCAATCAGGGTTCGAGTCAGTAGCTGGTAAGTACCAATT
  ACTAACTTCAGTTCTTCAGTGTTTGATGAAAATATTAACCATTGATTTAATAATCAACATCAAGAGGCAA
  CCTCAGAAAACTGCTGACCAAGAATCAGAAGACGAATTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027914
• Insert Size: 2142 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027914.1, NP_082190.1
• RefSeq Size: 2753 bp
• RefSeq ORF: 2142 bp
• Locus ID: 71769
• UniProt ID: Q9DBI2
• Cytogenetics: 10 D1
• Gene Summary: Probable molecular chaperone that assists the folding of proteins upon ATP hydrolysis. Plays a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation.[UniProtKB/Swiss-Prot Function]