Yme1l1 (NM_013771) Mouse Untagged Clone

CAT#: MC220981

Yme1l1 (untagged) - Mouse YME1-like 1 (S. cerevisiae) (Yme1l1), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Yme1l1
• Synonyms: Ftsh; FtsH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220981 representing NM_013771
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTCCCTGTCGAGCACTGTGCAGCCTCAGGTTACAATTCCTCTCAGTCATCTCATCAATGCTTTCC
  ATTCACCAAAAAATATATCTGTTTCTGTCAATACACCTGTTTCTCAAAAACAGCATCGAGATACAGTTCC
  TGAGCATGAAGCTCCCAGTAGTGAGCCTGTGCTTAATTTAAGGGACCTTGGATTATCTGAATTGAAAATT
  GGACAGATTGATAAAATGGTAGAAAATTTACTTCCTGGATTTTATAAAGACAAAAGAGTTTCTTCCTGTT
  GGCATACATCTCATATTTCGGCACAGTCCTTTTTTGAAAATAAATATGGTCACTTAGATATGTTCAGTAC
  ATTACGTTCCTCTAGCTTGTACAGGCAACATCCAAAAACTCTTCGAAGCATTTGTTCAGATCTTCAGTAT
  TTTCCAGTTTTCATACAGTCTCGGGGTTTCAAAACGTTGAAATCAAGGACACGACGTTTGCAGTCTACCT
  CTGAAAGATTAGTAGAAGCACAGAATATAGCACCATCATTTGTGAAGGGGTTTCTTTTGCGGGACAGAGG
  AACAGATCTTGAGAGTTTGGACAAACTTATGAAAACTAAAAACATACCTGAAGCTCACCAAGATGCATTT
  AAAACTGGTTTTGCAGAGGGTTTTCTCAAAGCTCAAGCTCTTACACAGAAGACCAATGATTCCTTAAGGC
  GAACTCGTCTGATCCTCTTTGTTTTGCTCCTGTTTGGCATTTATGGACTCTTAAAAAATCCGTTTTTATC
  TGTGCGCTTTCGGACAACTACAGGACTTGATTCTGCGGTAGACCCTGTCCAGATGAAAAATGTCACTTTT
  GAACATGTTAAAGGGGTGGAGGAAGCCAAACAAGAGTTACAGGAAGTGGTTGAATTCTTGAAAAATCCAC
  AGAAGTTTACTGTGCTTGGAGGTAAACTTCCCAAAGGAATTCTTTTAGTTGGGCCACCAGGAACAGGGAA
  GACGCTTCTTGCCCGAGCTGTGGCAGGAGAAGCTGACGTCCCTTTTTATTATGCTTCTGGATCAGAGTTT
  GATGAGATGTTTGTGGGCGTAGGAGCCAGCAGGATCAGAAATCTTTTTAGAGAAGCAAAAGCAAATGCTC
  CTTGTGTTATATTCATCGATGAATTAGATTCTGTTGGTGGAAAGAGAATTGAATCTCCAATGCACCCGTA
  TTCAAGGCAGACGATCAATCAGCTTCTTGCTGAAATGGATGGTTTCAAACCCAATGAAGGAGTAATCATT
  ATAGGTGCCACAAATTTCCCAGAGGCATTAGATAATGCCTTAATACGTCCTGGTCGTTTTGATATGCAAG
  TTACAGTTCCAAGGCCAGATGTGAAGGGTCGAACTGAAATTTTGAAATGGTATCTCAACAAGATAAAGTT
  TGATAAATCTGTTGATCCAGAAATCATAGCTCGAGGGACTGTTGGGTTCTCTGGAGCAGAGTTGGAGAAT
  CTTGTGAACCAAGCTGCACTAAAGGCAGCAGTTGATGGAAAAGAAATGGTTACCATGAAGGAACTAGAGT
  TTTCCAAGGATAAAATTCTAATGGGACCAGAAAGAAGAAGTGTGGAAATTGATAACAAAAACAAAACTAT
  AACAGCCTATCATGAATCTGGTCATGCTATTATTGCATATTACACAAAGGATGCAATGCCAATTAATAAA
  GCTACAATCATGCCTCGAGGGCCAACACTTGGGCATGTATCACTGTTGCCTGAGAATGACAGATGGAATG
  AAACTCGAGCCCAGCTGCTTGCACAGATGGATGTTAGCATGGGAGGAAGAGTGGCAGAGGAACTCATATT
  TGGAACTGATCATATTACAACTGGTGCTTCTAGTGATTTTGATAATGCAACAAAAATTGCTAAGAGGATG
  GTTACCAAATTTGGAATGAGTGAAAAGCTTGGAGTTATGACCTACAGTGATACAGGAAAACTAAGTCCTG
  AAACTCAATCAGCCATTGAACAAGAAATAAGAATCCTTCTACGGGAGTCATATGAACGAGCAAAACATAT
  CTTGAAAACACATGCGAAAGAACATAAGAACCTGGCAGAAGCATTGCTGACCTATGAGACTTTGGATGCC
  AAAGAGATTCAAATTGTTCTTGAGGGAAAGAAATTGGAAGTGAGATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013771
• Insert Size: 2148 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013771.5, NP_038799.1
• RefSeq Size: 4571 bp
• RefSeq ORF: 2148 bp
• Locus ID: 27377
• UniProt ID: O88967
• Cytogenetics: 2 A3
• Gene Summary: ATP-dependent metalloprotease that catalyzes the degradation of folded and unfolded proteins with a suitable degron sequence in the mitochondrial intermembrane region (By similarity). Plays an important role in regulating mitochondrial morphology and function by cleaving OPA1 at position S2, giving rise to a form of OPA1 that promotes maintenance of normal mitochondrial structure (PubMed:17709429, PubMed:24616225, PubMed:26785494, PubMed:27495975). Ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins (By similarity). Required for normal, constitutive degradation of PRELID1 (PubMed:26785494). Catalyzes the degradation of OMA1 in response to membrane depolarization. Required to control the accumulation of nonassembled respiratory chain subunits (NDUFB6, OX4 and ND1) (By similarity).[UniProtKB/Swiss-Prot Function]