Trappc12 (NM_178811) Mouse Untagged Clone

CAT#: MC220970

Trappc12 (untagged) - Mouse tetratricopeptide repeat domain 15 (Ttc15), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trappc12
• Synonyms: CGI-87; D930014A20Rik; Ttc15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220970 representing NM_178811
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACAGCTAAAGATGGTGAGCAGAGCCCAAGTGAGGCATCTCCTTCAGCCCAGGCAGGTCCTGAAA
  ACATCCCGGAGCCTATGTCTCGGGAGGAGGAGAGCCAGCCATTGTACCATGAAGAGACCATTGATCTTGG
  TGGAGATGAGTTTGCATCTGAAGAGAACGAGCCCACATCGGAAGGGTCTCACAACTTCGGAGATAAGCTT
  AATGAGCACATGATGGAAAGTGTGCTCATCTCCGACTCTCCTAACAACAGCGAGGGGGACGTTGGCGACC
  TCGGCTGTCTACAGGATGTTGGGGAGCCCCCCAGAGGTGCCACTGATCACCGACTGCCCAGTAGCACAGA
  CAAAGAAGTGGTGGACACTCTGAGTAATGGCAGTGAAACCGATGGAGACGACACTCCCAGAGACATCTCA
  GACATGACACCTGATAGCAGAGCCTCTCTGAAGGAAGACTCAACACAGGAAGATGTGACGAGCATGCCTG
  CACTGGAGAATGCTGCCACGGAGGAAGTCGGGCCTAAGGACAGCCTCGCGCCCCGAGAAGAGCAGACCTC
  AGAAGTGTCCTCCAATCAGTCCTCCTCCAAAGACGAGCCCTTGCCCGTCTGCACCATCTTCAGCCAGGCT
  ACCGCCACTCCCTCCCAGCCACACCTGTTTCTACAGGATGGCTTCGAGTCCCAGATGGTGAAATCACCCA
  GTTTCAGTAGCACCAGTGAGACGTCAGCCAAGACCCCTCCTCCGATGGTCCAGCCCAGTCCCAGCCTCAG
  TACATTTTTTGGAGATACAATGAGCTCTAATTCCTTGGCCTCTGACTTCTTTGATTCCTTCACAACTTCT
  ACTTTCATTTCAGTCAGTAATCCCAATGCAGGTTCCCCAGTTCCAGAAAAGCTGTCTTCACTCACTGCCC
  CTGTAGGAGAGAAGTCCCCGGATTCCACCAGCCCATCTTACTCCACAAGGATGGATAGATCGGAATCAGG
  TGTTTCTCGGGCCCCTCTGGATGTCCCTGAATCTCCAAAGCCATTTTCACAGATCCAGGCTGTGTTTGCT
  GGAAGTGATGACCCCTTCGCCACAGCCCTGAGCATGAGTGAGATGGACCGGAGGAATGACGCATGGCTTC
  CCAGTCAGGCGACCAGAGAAGTCCTGATGTCAGTAGCAACCCAGCAGTATGGCACTGTGTTCATAGACAA
  GGAGAACCTCACTATGCCTGGCCTCAAGTTTGACAACATTCAGGGAGATGCAGTTAAAGACTTGATGCTT
  CGCTTCCTGGGTGAGAAGGCTGCAGCAAAGAGACAGGTCTTAAATGCCAGCTCAGTGGAGCAGTCCTTTG
  TGGGGCTGAAGCAGCTCATTAGCTGCAGGAACTGGAGGGCAGCAGTCGACCTGTGTGGACGCCTGCTCAC
  AGCCCACGGACAAGGCTATGGCAAGAATGGGCTGCCCACCAGCCACACCACAGACTCCTTGCAGCTCTGG
  TTTGTGAGGTTGGCGCTGCTGGTGAAGTTGGGCCTTTTCCAGAATGCTGAGATGGAGTTTGAACCCTTTG
  GAAATCTGGATCAACCAGACCTATACTATGAATACTACCCACACGTGTACCCAGGACGCAGGGGCTCCAT
  GGTCCCCTTTTCGATGCGTATTCTGCATGCAGAACTGCAGCAGTATCTGGGGAACCCACAGGAGTCCCTG
  GACCGATTACACAGGGTCAAGACCGTCTGCAGCAAGATCTTGGCCAACTTGGAGCAAGGCTTAGCTGAAG
  ATGGTGGTCTGAGCAGCGTCACCCAGGAAAGCAGGCAAGCCTCTGTTCAGCTGTGGAGGTCTCGTCTGGG
  CCGTGTGCTGTACTCCATGGCAAACTGTCTGCTCCTGATGAAGGACTATGTGCTGGCTGTGGATGCCTAC
  CTCACAGTAATCAAGTACTACCCGGAGCAGGAGCCACAGCTGCTGAGTGGAATCGGCCGCATTCTCCTCC
  AGATCGGAGACATAAAAACAGCTGAAAAATATTTTCAAGACGTTGAGAAAGTAACTCAGAAGTTGGATGG
  ACTGCAGGGTAAAATAATGGTCCTAATGAACAGAGCCTTTCTGTACCTTGGCCAGAATAACTTCGCAGAA
  GCTCACAAGTTCTTCACAGAGATTTTGAGAATGGACCCAACAAATGCAGTGGCCAACAACAATGCTGCTG
  TGTGTCTGCTTTACCTGGGAAAGCTCAAGGACTCCCTGCGGCAGCTGGAGGCCATGGTGCAGCAGGACCC
  CAGGCACTACCTGCACGAGAGCGTGCTCTTCAACCTGACCACCATGTACGAGCTGGAGTCCTCAAGGAGC
  ATGCAAAAGAAGCAGAGCCTACTTGAGGCCGTTGCTAGCAAAGAAGGGGACAGCTTCAACACACAGTGCC
  TGAAGCTGGCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178811
• Insert Size: 2394 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178811.4, NP_848926.3
• RefSeq Size: 3394 bp
• RefSeq ORF: 2394 bp
• Locus ID: 217449
• UniProt ID: Q8K2L8
• Cytogenetics: 12 A2
• Gene Summary: Component of the TRAPP complex, which is involved in endoplasmic reticulum to Golgi apparatus trafficking at a very early stage. Also plays a role in chromosome congression, kinetochore assembly and stability and controls the recruitment of CENPE to the kinetochores.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.