Itih4 (NM_001159299) Mouse Untagged Clone

CAT#: MC220968

Itih4 (untagged) - Mouse inter alpha-trypsin inhibitor, heavy chain 4 (Itih4), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Itih4
• Synonyms: ITI-HC4; Itih; Itih-4; PK-120
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220968 representing NM_001159299
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGCCCTGCCCCTGCCCACATGTGGAACCTTGTGCTGTTCTTGCCTTCACTGTTGGCTGTGCTTC
  CGACCACTACTGCCGAGAAGAATGGCATCGATATCTACAGCCTCACGGTGGACTCCCGGGTCTCTTCCCG
  ATTTGCCCATACTGTTGTCACCAGCCGGGTGGTCAACAGAGCCGATGCTGTTCAAGAAGCGACCTTCCAA
  GTAGAGCTACCCAGGAAAGCCTTCATCACCAACTTTTCCATGATCATCGATGGCGTGACCTACCCAGGGG
  TTGTCAAAGAGAAGGCCGAAGCCCAGAAACAATACAGTGCCGCCGTGGGCAGGGGAGAGAGTGCTGGCAT
  CGTCAAGACCACTGGGAGGCAGACAGAGAAGTTTGAAGTGTCAGTCAACGTGGCCCCTGGTTCCAAGATT
  ACCTTCGAACTCATATACCAGGAACTGCTCCAAAGGCGACTGGGAATGTATGAGCTACTCCTCAAAGTGA
  GGCCTCAGCAGCTGGTGAAGCACCTTCAGATGGACATCTACATCTTTGAGCCTCAGGGTATTAGCATCCT
  GGAGACAGAGAGCACCTTCATGACCCCGGAGCTGGCAAATGCCCTTACCACTTCACAGAACAAGACCAAG
  GCTCATATCCGGTTCAAGCCGACGCTCTCCCAGCAACAGAAGTCTCAGAGTGAGCAGGACACGGTGCTGA
  ATGGGGACTTCATCGTCCGCTATGATGTCAACCGGTCTGACTCTGGGGGCTCCATTCAGATTGAGGAAGG
  CTACTTTGTGCACCACTTTGCTCCAGAGAACCTTCCTACAATGTCCAAGAATGTGATCTTTGTCATTGAT
  AAAAGCGGATCTATGTCAGGCAAGAAAATCCAGCAGACCCGAGAAGCCCTAGTCAAGATCTTGAAAGACC
  TCAGCCCCCAAGACCAGTTCAACCTCATTGAGTTCAGTGGGGAAGCAAACCAATGGAAGCAGTCACTGGT
  GCAAGCGACAGAAGAGAATTTGAACAAGGCTGTAAACTATGCTTCCAGGATCCGGGCTCACGGAGGGACC
  AACATCAATAATGCAGTGCTGTTGGCTGTGGAGCTGCTGGACAGAAGCAACCAAGCTGAGCTACTGCCCT
  CGAAGAGCGTCTCCCTTATCATCCTGCTCACGGACGGTGACCCCACTGTGGGAGAAACCAACCCCACGAT
  TATCCAGAACAACGTGCGGGAAGCCATCAATGGGCAGTATAGCCTCTTCTGCCTGGGGTTCGGCTTTGAT
  GTGAACTATCCTTTCCTGGAGAAGATGGCACTGGACAATGGTGGCCTGGCCAGGCGCATCTATGAGGATT
  CAGACTCTGCACTGCAGCTTCAGGATTTCTACCACGAAGTAGCCAATCCACTGCTCTCATCAGTGGCCTT
  CGAATACCCCAGTGATGCTGTGGAGGAAGTCACTCGGTACAAGTTCCAACACCACTTTAAGGGCTCAGAG
  ATGGTGGTGGCTGGGAAGCTCCAGGACCAGGGTCCTGATGTCCTCTTAGCCAAAGTCAGTGGGCAGATGC
  ACATGCAGAACATCACTTTCCAAACGGAGGCCAGCGTAGCCCAACAAGAGAAGGAGTTTAAGAGCCCCAA
  GTACATCTTTCACAACTTTATGGAGAGACTGTGGGCACTGCTGACTATACAGCAACAGCTGGAGCAGAGG
  ATTTCAGCGTCAGGTGCCGAATTAGAGGCCCTCGAGGCCCAAGTTCTGAACTTGTCACTCAAGTACAATT
  TTGTCACCCCTCTCACGCACATGGTGGTCACCAAACCTGAAGGTCAAGAACAATTCCAAGTTGCTGAGAA
  GCCTGTGGAAGTCGGTGATGGCATGCAGAGACTCCCCTTAGCAGCTCAAGCCCACCCCTTCAGGCCTCCT
  GTCAGAGGATCTAAACTGATGACCGTGCTGAAAGGAAGCAGGTCCCAGATACCCAGACTCGGTGATGCCG
  TTAGGGCATCTAGGCAATACATTCCTCCCGGATTCCCCGGACCTCCTGGACCTCCCGGATTTCCTGCACC
  TCCTGGACCTCCTGGATTTCCTGCACCCCCTGGACCTCCTCTTGCTTCTGGCTCTGACTTCAGCCTTCAG
  CCTTCCTATGAAAGGATGCTAAGCCTGCCCTCCGTTGCACAATATCCTGCTGACCCACATCTGGTTGTGA
  CGGAAAAAAGTAAAGAAAGCACCATACCAGAGGAATCCCCAAACCCAGACCACCCCCAGGTTCCTACTAT
  TACCTTGCCGCTTCCGGGATCCAGTGTGGACCAGCTCTGTGTGGATATCTTACATTCTGAGAAGCCCATG
  AAGCTGTTCGTAGACCCCAGTCAGGGTCTGGAGGTGACTGGTAAGTATGAGAATACTGGGTTCTCGTGGC
  TCGAAGTGACCATCCAGAAGCCTCACCTGCAGGTCCATGCAACCCCTGAACGACTGGTGGTGACACGAGG
  CAGAAAAAACACTGAATACAAGTGGAAGAAGACGCTGTTCTCTGTGTTACCTGGCTTGAAGATGACCATG
  AATATGATGGGACTCCTACAGCTCAGTGGCCCAGACAAAGTCACCATCGGCCTCCTGTCCCTGGATGACC
  CTCAGAGAGGACTAATGCTGCTTTTGAATGACACCCAGCACTTCTCCAACAACGTTAAAGGGGAGCTTGG
  TCAGTTTTACCGGGACATCGTCTGGGAGCCACCCGTCGAGCCAGATAATACAAAACGGACAGTCAAAGTT
  CAAGGAGTTGACTACCTGGCTACCAGAGAGCTCAAGTTGAGTTACCAAGAAGGGTTCCCAGGAGCAGAGA
  TTTCCTGCTGGACAGTGGAGATATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159299
• Insert Size: 2826 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159299.2, NP_001152771.1
• RefSeq Size: 3161 bp
• RefSeq ORF: 2826 bp
• Locus ID: 16427
• UniProt ID: A6X935
• Cytogenetics: 14 19.09 cM
• Gene Summary: This gene encodes a member of the inter-alpha trypsin inhibitor (IaI) family of plasma serine protease inhibitors with diverse functions as anti-apoptotic and matrix stabilizing molecules during development. This gene is predominantly expressed in the liver and the encoded protein was found to be a plasma kallikrein-sensitive glycoprotein. This gene is located in a cluster of related inter alpha trypsin inhibitor genes on chromosome 14. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This difference results in a protein (isoform 2) that is 1 amino acid shorter than isoform 1.