Mthfd1l (BC030437) Mouse Untagged Clone

CAT#: MC220946

Mthfd1l (untagged) - Mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (cDNA clone MGC:40924 IMAGE:5374413),, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mthfd1l
• Synonyms: 2410004L15Rik; AI647056; Fthfsdc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC030437
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAATGAGCTGCCCCTGGGCAACACCTCAGCTTCCAGACAAGCTTCGAGAGGCAGACATTGTGGTCT
  TAGGCTCACCTAAGCCAGAAGAGATTCCTGCTGCGTGGATTCCATCAGGAACCACCATTCTCAATTGCTT
  CCATGACTTCTTGTCAGGGAAGCTGAGCGGAGGTTCTCCTGGGGTCCCCGTGGACAAACTCATTGCAGAA
  GAGAGCGTGAGTCTCCTTGCTGCGGCTCTGCGCATTCAGAACATGGTGAGCAGCGGCAGGAGGTGGCTCA
  GAGAGCAGCAACATCGAAGATGGCGACTGCACTGCTTGAAACTGCAGCCTCTCTCACCTGTGCCCAGTGA
  TATTGAGATTTCTCGAGGACAGACTCCAAAAGCTGTGGATGTCCTTGCCAAGGAGATAGGATTGCTTGCA
  GATGAAATTGAAATCTATGGCAAGAGCAAAGCCAAAGTCCAATTGTCCCTGCTGGAGAGGTTAAAGGATC
  AAACAGATGGAAAATACGTCTTGGTTGCTGGGATCACGCCCACCCCGCTCGGAGAAGGGAAGAGTACAGT
  CACCATCGGACTAGTGCAGGCGCTGACCGCTCACCTGAAAGTCAACTCCTTCGCCTGTCTGAGGCAGCCT
  TCTCAGGGACCCACTTTTGGAGTGAAAGGAGGAGCTGCAGGAGGTGGATATGCTCAGGTCATCCCCATGG
  AGGAGTTCAACCTTCACCTGACTGGGGACATCCATGCCATCACTGCCGCTAATAACTTGCTGGCCGCAGC
  CATCGACACGAGAATTTTACATGAGAGCACTCAAACAGACAAGGCTCTCTACAATCGACTGGTTCCCTTA
  GTGAATGGTGTCAGAGAATTTTCAGAAATTCAACTTTCTCGGCTGAAGAAACTGGGGATACATAAGACTG
  ATCCAAGCACACTGACCGAGGAGGAAGTGAGGAAGTTCGCCCGCCTCAACATTGACCCCGCAACCATCAC
  GTGGCAGAGAGTATTGGACACGAACGACCGATTTCTGAGAAAAATAACCATTGGACAGGGAAGTACAGAG
  AAAGGATATTCCCGGCAGGCACAGTTTGACATTGCAGTGGCCAGCGAGATCATGGCCGTGCTGGCCTTGA
  CTGATAGCCTCACAGACATGAAAGAGCGGCTAGGAAGAATGGTGGTGGCCAGTGACAAAGACGGGCAGCC
  TGTGACAGCTGAGGACTTGGGGGTGACCGGCGCTCTGACAGTTTTGATGAAAGACGCCATCAAGCCAAAT
  CTGATGCAGACCCTTGAAGGAACACCTGTGTTTGTGCATGCTGGTCCCTTTGCCAACATCGCTCACGGCA
  ACTCATCAGTATTAGCTGATAAAATCGCGCTGAAATTGGTCGGCGAAGAAGGGTTTGTGGTGACTGAAGC
  TGGCTTTGGGGCTGATATTGGGATGGAGAAATTCTTCAATATCAAATGCCGAGCTTCTGGCCTGGTGCCC
  AACGTTGTCGTGCTGGTAGCCACCGTTCGAGCTCTGAAGATGCATGGAGGCGGGCCGAGTGTAACTGCTG
  GGGTTCCTCTCAAGAAAGAATATACAGAGGAGAACATTCAGCTGGTAGCTGATGGCTGCTGCAACCTACA
  GAAGCAAATTCAGATCGCTCAGCTCTTCGGGGTGCCGGTGGTGGTGGCACTGAATGTCTTCAAGACGGAC
  ACCCGTGCAGAGATTGACTTGGTGTGTGAGCTCGCTAAACGGGCCGGTGCCTTTGATGCGGTCCCCTGCT
  ATCACTGGTCGGCTGGAGGGAAAGGATCTGTGGACTTGGCCCGGGCTGTGAGAGAAGCTGCGAATAAAAG
  AAGCCGCTTCCAGTTCCTGTATGATGTTCAGCTTCCCATCGTGGAAAAGATAAGAGTCATTGCCCAGACT
  GTCTATGGAGCCAAGGACATTGAGCTGTCTCCTGAGGCGCAGTCCAAAATCGATCGCTATACGCAACAGG
  GTTTTGGAAATTTGCCCATTTGCATGGCGAAGACCCACCTCTCTCTCTCTCATGAACCTGACAAAAAGGG
  AGTGCCGAGGGATTTCACTCTGCCCATCAGTGATGTCCGGGCCAGCATCGGGGCTGGGTTCATTTATCCT
  TTGGTGGGAACGATGAGCACAATGCCAGGACTGCCCACCCGACCCTGCTTTTATGATATAGACCTTGACA
  CAGAAACGGAGCAAGTTAAAGGCTTGTTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC030437
• Insert Size: 2202 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC030437, AAH30437
• RefSeq Size: 3061 bp
• RefSeq ORF: 2201 bp
• Locus ID: 270685
• Cytogenetics: 10 A1
• Gene Summary: May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2.[UniProtKB/Swiss-Prot Function]