Ankrd27 (BC065093) Mouse Untagged Clone

CAT#: MC220935

Ankrd27 (untagged) - Mouse ankyrin repeat domain 27 (VPS9 domain) (cDNA clone MGC:85985 IMAGE:6855501), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ankrd27
• Synonyms: MGC25907, Varp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC065093
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTATATGATGAAGATCTCCTGAAAAACCCTTTCTACCTGGCCCTTCAGAAGTGGCGCCCCGACT
  TGTGCAGCAAGGTGGCCCAGATCCATGGCATTGTCCTAGTGCCTTGCAGAGGAAGCCTGCCCGGCAGTGT
  GCAGGCCTCCTGCCAGTTCGAGTCCTATGTTTTGGTACCCACGGAAGGACACTTTCAGACCTTAGATGGA
  AAGGCTGTCGTCATTGAAGGAAACAGGATTAAGCTAGGAGCAGGTTTTGCTTGCCTTCTCTCTGTGCCCA
  TCCTCTTTGAGGAGACTTTCTACAACGAGAAAGAGGAGAGTTTCAGCATTCTCTGCATTGCTCATCCTTT
  GGAGAGGAGAGAGACTTCAGAAGAACCTTCAGCGCCTGCAGATCCTTTCTCCCTGAAAACCATCGAAGAT
  GTGAGAGAATTTTTGGGAAGACACTCAGAGAAATTTGACAAGAACATTGCCTCTTTCCACCGGACGTTCC
  GAGAATGTGAAAGAAAGAGCCTCCGCCACCACATAGACTCCGTAAATGCTCTCTACACCAAATGCCTCCA
  GCAGCTTCTCCGAGACTCTCACCTGAAGGTTCTTGCAAAGCAGGAGGCCCAGATGAACCTGATGAAGCAG
  GCCGTAGAGATGTATGTCCATCACGATATTTACGACCTGATCTTTAAATACGTGGGGACCATGGAGGCGA
  GCGAGGATGCCGCCTTTAACAAAATCACGAGAAGCCTTCAAGATCTCCAGCAGAAAGACATCGGCGTGAA
  ACCCGAGTTCAGCTTCAACATCCCTCGGGCAAAGAGAGAGCTGGGTCAGTTGAACAAGTGTACGTCGCCA
  CAGCAGAAGCTGCTGTGCCTGAGGAAGGTGGTCCAGCTCATGACACAATCTCCCAGCCAGAGAGTGAACT
  TGGAGACCATGTGTGCCGATGATCTCCTCTCGGTCCTGTTATATCTGCTCGTGAAGACAGAGATCCCTAA
  CTGGATGGCAAATTTGAGCTACATCAAAAACTTCAGATTTAGCAGCTCGGCCAAAGATGAGCTAGGATAC
  TGCCTGACCTCAGTCGAGGCTGCGATTGAATACATCCGGCAAGGAAGTCTCTCCACGAAGACGCCTGACG
  CTGAGGGATTTGGCGACAGACTATTTCTCAAGCAGCGGATGAACTTGCTGTCCCAGATGACATCAACACC
  CATCGACTGCCTGTTCAAGCACATCGCCTCTGGTAACCAAAAGGAAGTGGAAAGACTTCTGAGCCAAGAT
  GACCAGGACAAAGACGCCATGCAAAAGATGTGCCACCCACTTTGTTCCTGTGAGGACTGTGAGAAGCTTA
  TCTCTGGGAGGCTGAATGACCCGTCAGTTGTCACTCCTTTCTCCAGAGATGACAGAGGTCAAACACCCCT
  CCATGTGGCTGCCCTCTGTGGTCAGGCATCCCTCATTGACTTCCTGGTCTCCAAAGGTGCCGTAGTGAAC
  GCCACAGACTATCATGGGTCCACCCCCTTACATCTGGCATGTCAGAAAGGCTTCCAAAGTGTGACGCTGC
  TGCTGCTGCACTACAAGGCCAGCACTGAGGTTCAGGACAACAACGGCAACACCCCGCTGCACCTGGCCTG
  CACCTATGGGCAGGAGGATTGCGTGAAGGCCCTGGTCTACTATGATGTCCAGGCCTGCAGATTGGATATT
  GGTAATGAGAAAGGAGACACAGCCCTGCACATTGCTGCACGCTGGGGTTACGAGGGCATCATAGAGACAC
  TGCTGCAGAATGGAGCCCCCACAGCAGTCCAGAACAGACTGAAGGAAACACCACTCAAGTGTGCTCTGAA
  CTCGAAGATTCTGTCTATCATGGAAGCTCATCACCTATCCTCTGACAGGAGGCCAAGGCCTTCTGAGGTT
  CCTGCACAGTCCCCTACACGCTCTGTGGATTCCATCAGCCAGGGCTCCTCCACCTCCAGCTTTTCTTCCA
  TATCGGTGAGCTTCAGGCAAGAAGAGGTCAAAAAGGACTACAGGGAGAAACTGGCCAGGATTTCTGCCAA
  TGGGCTTAGTGTGAATGTGACCAACCAGGACGGCTTCTCTCCCCTGCACATGGCTGCCCTGCATGGCCGC
  ACAGACCTCGTCCCCCTCCTGCTGAAGCACGGTGCTTACTCAGGTGCCAGAAACACAAGCCAAGCCGTCC
  CGCTCCACCTGGCCTGCCAGCAGGGGCACTTTCAGGTGGCAAAATGTCTCTTAGATTCCAATGCAAAACC
  TAATAAAAAGGACCTCAGTGGGAACACACCCCTCATTTGTGCCTGCTCTGCCGGCCACCATGAAGTCGCT
  GCGCTGTTGCTACAGCACGGGGCCTCCATCAATGCTTGCAACAACAAAGGCAACACGGCCCTGCATGAGG
  CTGTGATGGGAAGGCACACCCTGGTAGTGGAGCTGCTTCTGTTCTACGGGGCGTCGGTTGACATCCTGAA
  CAAGAGGCAGTACACGGCGGCGGACTGTGCCGAGCAGGACTCAAAAATAATGGAGTTGCTGCAGGTAGTG
  CCAGGCTGTGTTGCGTCTCTAGACAGCGTCGAGGAGGCAGACCACGAGGGTTACGTGACTGTTGAGATCA
  GGAGAAAATGGAACCCCAAAATGTACAACCTGCCAGAGGAACCTCTTAGGAGACAGTTTTGCCTCATTAA
  CCCGGGGGGTCGGTTCCAGGAGAGGACATCCAGGGAGACTATGGGAAGGGATAGAAGTGTGCCCGATCTT
  GCAGGACGTTCTCTTCAGGAGCCAGAGAAGCAAAGAGTCACCGGAAAGCAGAGTGACCTGTCAGACCTGA
  GCAGGTATCAGACCTCTGAGGAAGGAAACAAGGGGCTCCCGGAGAGGCCCGTGTCGAGACAGGCTGCTCC
  AGGACACCGGCCGATGGTCCGCAGACACACAGTCAATGACGCAGCCATACTCCAGGTCCCAGAGGTGACT
  GTCCACCTCACCACCCACGAGGCGAGTGTTCCTCAGTCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC065093
• Insert Size: 2982 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC065093, AAH65093
• RefSeq Size: 4105 bp
• RefSeq ORF: 2981 bp
• Locus ID: 245886
• Cytogenetics: 7 B2
• Gene Summary: May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics (By similarity). May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation (By similarity). Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7 (PubMed:19403694, PubMed:21187289). Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38 (PubMed:19745841, PubMed:22171327). Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1. Required for the colocalization of VAMP7 and Rab21, probably on TGN sites (By similarity). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with VPS29 (By similarity). Regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (PubMed:26620560).[UniProtKB/Swiss-Prot Function]