Wwc1 (BC037006) Mouse Untagged Clone

CAT#: MC220895

Wwc1 (untagged) - Mouse WW, C2 and coiled-coil domain containing 1 (cDNA clone MGC:47054 IMAGE:5116674), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wwc1
• Synonyms: MGC47054, mKIAA0869, RP23-196B5.5, Kibra
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC037006
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTCCCTGTCTTCAGGCAGTAGCCCTGGGTCACTCACCTCCAGCCGGGGCTCCCTGGCCGCCTCTA
  GCCTGGACTCCTCCACCTCAGCCAGCTTCACTGACCTCTATTATGACCCGTTCGAGCAGTTGGACTCGGA
  GCTCCAGAGCAAGGTGGAGCTGCTGTTCCTGGAGGGTGCCACGGGCTTCCGGCCCTCAGGCTGCATCACC
  ACCATCCACGAGGATGAGGTGGCCAAGACCCAGAAGGCAGAGGGAGGCAGCCGCCTGCAGGCCCTGCGCT
  CCCTATCTGGCACTCCAAGGTCCATGACCTCCCTGTCTCCACGCTCATCTCTCTCCTCCCCATCCCCTCC
  CTGTTCCCCTCTCATCACTGATCCCCTCCTGACTGGAGATGCCTTCCTGGCCCCGCTGGAGTTTGAAGAC
  ACAGAGCTGAGTACCACTCTCTGTGAGCTGAACCTCGGCGGTAGCGGCACCCAGGAAAGATACCGGCTGG
  AGGAACCAGGACCTGAGGGCAAGCCACTGGGCCAAGCTGCAAGTGTGGCCCCAGGATGTGGCCTCAAAGT
  AGCCTGTGTGTCCGCTGCAGTGTCCGACGAGTCAGTAGCCGGGGACAGTGGCGTGTATGAGGCTTCAGCA
  CAGAGACCAGGTACTTCTGAAGCTGCCGCATTCGACAGTGATGAATCGGAAGCTGTGGGTGCCACTCGGG
  TTCAGATTGCCCTGAAATATGATGAGAAGAATAAGCAATTTGCAATATTAATCATTCAGCTGAGTCACCT
  CTCTGCTCTGTCACTACAACAAGACCAGAAAGTGAACATCCGTGTAGCCATCCTGCCTTGCTCTGAAAGC
  AGCACCTGCTTGTTCAGAACCCGGCCTCTGGACTCGGCAAATACCCTCGTGTTCAATGAGGCATTCTGGG
  TGTCCATCTCCTACCCAGCCCTTCACCAAAAGACCTTACGAGTCGATGTCTGCACCACTGACAGGAGCCA
  TACAGAGGAGTGTCTGGGAGGTGCCCAGATCAGCCTGGCCGAGGTCTGCCGGTCTGGGGAGAGGTCAACG
  CGCTGGTACAATCTCCTGAGCTACAAATACTTGAAGAAGCAGTGCAGGGAGCCCCAGCCAACAGAAGCTC
  CAGGGCCAGACCATGTGGACGCTGTGTCTGCCTTGCTAGAACAGACAGCCGTGGAGCTAGAGAAGAGGCA
  GGAGGGAAGAAGCAGCTCCCAGACGCTGGAGGGCAGCTGGACGTACGAGGAAGAGGCCAGCGAGAATGAG
  GCAGTCGCTGAGGAGGAAGAGGAGGGAGAGGAAGATGTCTTCACTGAAAAGGTCTCTCCCGAGGCAGAGG
  AGTGCCCAGCATTAAAGGTGGACAGAGAGACCAATACTGACAGCGTGGCCCCATCCCCCACAGTGGTGCG
  CCCTAAGGACCGGAGGGTGGGTGCCCCGTCGACAGGGCCATTTCTTCGAGGGAATACCATCATCCGCTCC
  AAGACCTTCTCGCCTGGACCCCAGAGCCAGTATGTGTGTCGGCTAAACCGCAGTGACAGTGATAGTTCTA
  CGCTGTCCAAGAAGCCACCTTTTGTGCGAAACTCCCTGGAGCGGCGCAGCGTCAGGATGAAGCGGCCTTC
  CTCCGTCAAGTCCCTGCGCACAGAGCGTCTGATCCGCACCTCGCTGGACCTAGAACTCGACCTGCAGGCC
  ACGAGGACCTGGCACAGCCAGCTGACTCAGGAGATCTCAGTGCTGAAGGAGCTGAAGGAGCATTTGGAGC
  AAGCCAAGAACCACGGGGAGAAGGAGCTGCCACAGTGGCTGCGTGAAGACGAGCGCTTCCGCTTGCTACT
  GAGGATGCTGGAGAAGAAGGTGGATCGTGGGGAGCACAAGAGTGAGTTGCAAGCCGACAAGATGATGAGA
  GCTGCTGCCAAGGATGTGCACAGGCTTCGAGGCCAGAGCTGTAAGGAGCCCCCAGAAGTGCAGTCTTTCA
  GGGAGAAAATGGCATTTTTCACCCGGCCTCGGATGAACATCCCAGCTCTCTCTGCAGATGACGTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC037006
• Insert Size: 2028 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC037006, AAH37006
• RefSeq Size: 2583 bp
• RefSeq ORF: 2027 bp
• Locus ID: 211652
• Cytogenetics: 11 A4
• Gene Summary: Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with NF2 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. Acts as a transcriptional coactivator of ESR1 which plays an essential role in DYNLL1-mediated ESR1 transactivation. Regulates collagen-stimulated activation of the ERK/MAPK cascade. Modulates directional migration of podocytes. Acts as a substrate for PRKCZ and may be associated with memory performance (By similarity). Regulates collagen-stimulated activation of the ERK/MAPK cascade.[UniProtKB/Swiss-Prot Function]