Slc8a3 (NM_001167920) Mouse Untagged Clone

CAT#: MC220884

Slc8a3 (untagged) - Mouse solute carrier family 8 (sodium/calcium exchanger), member 3 (Slc8a3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc8a3
• Synonyms: AW742262; Ncx3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220884 representing NM_001167920
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTGGTTACGGCTGCAGCCTCTCACCTCTGCCTTCCTCCATTTTGGGCTGGTTACTTTTGTGCTCT
  TCCTGAATTGTCTTCGAGCAGAGGCTGGTGACTCGGGGGATGTGCCCAGTGCAGGGCAGAACAATGAGTC
  CTGTTCGGGGTCATCAGACTGCAAGGAGGGTGTCATTTTGCCAATCTGGTATCCAGAGAACCCTTCCCTT
  GGGGACAAGATTGCCAGGGTCATTGTCTATTTTGTGGCCCTGATATACATGTTTCTTGGGGTGTCTATCA
  TTGCTGACCGATTCATGGCATCTATTGAAGTCATTACTTCCCAAGAGAGGGAAGTGACCATCAAGAAGCC
  CAATGGAGAGACCAGCACAACTACAATTCGGGTATGGAATGAAACTGTCTCCAATCTGACCCTGATGGCC
  CTGGGCTCTTCTGCTCCAGAGATTCTCCTGTCTTTAATTGAGGTGTGTGGTCACGGGTTCATTGCTGGTG
  ATCTGGGACCATCTACCATCGTTGGCAGTGCAGCCTTCAACATGTTCATCATCATTGGCATCTGTGTCTA
  TGTGATCCCAGATGGGGAGACTCGAAAGATCAAGCACCTGCGAGTCTTCTTCGTCACGGCTGCTTGGAGC
  ATCTTCGCCTACATTTGGCTCTATATGATCCTGGCAGTCTTCTCTCCTGGTGTGGTCCAGGTTTGGGAAG
  GCCTCCTTACTCTCTTCTTCTTTCCCGTGTGTGTCCTGCTGGCTTGGGTGGCAGATAAGCGACTGCTCTT
  CTACAAATACATGCACAAAAAATACCGCACAGATAAACACCGAGGAATTATCATTGAGACAGAGGGTGAC
  CACCCTAAGGGCATTGAGATGGATGGGAAAATGATGAATTCTCACTTTCTAGATGGGAACTTTACACCTT
  TGGAAGGAAAGGAGGTAGATGAATCTCGCAGGGAAATGATCCGGATTCTAAAGGATCTGAAACAAAAACA
  CCCAGAAAAGGACCTAGATCAGCTGGTGGAGATGGCCAATTACTATGCTCTTTCCCATCAACAGAAGAGC
  CGTGCTTTCTACCGCATCCAAGCCACCCGGATGATGACTGGTGCGGGCAATATACTTAAGAAGCATGCAG
  CCGAGCAAGCCAAGAAGACCTCCAGCATGAGCGAGGTGCATACCGATGAGCCGGAGGACTTTGCCTCTAA
  GGTCTTCTTTGACCCATGTTCTTATCAGTGCCTGGAGAACTGTGGAGCTGTCCTCCTGACCGTGGTGAGG
  AAAGGGGGAGATATATCCAAGACCATGTACGTGGACTACAAAACAGAGGACGGCTCCGCCAATGCAGGGG
  CAGACTATGAGTTCACAGAGGGCACTGTGGTTCTGAAGCCAGGAGAGACCCAGAAGGAGTTCTCTGTGGG
  CATCATTGATGATGACATTTTTGAGGAGGATGAACACTTCTTTGTGAGGCTGAGCAATGTCCGTGTAGAA
  GAGGAGCAGCTGGCGGAGGGGATGCTCCCAGCAATACTCAATAGTCTTCCTTTGCCTCGGGCTGTCCTGG
  CCTCCCCTTGTGTGGCCACAGTAACCATCTTGGATGATGACCATGCAGGAATTTTCACTTTTGAATGTGA
  TACCATTCATGTCAGTGAAAGTATTGGTGTTATGGAAGTCAAGGTTTTGAGGACATCAGGTGCCAGGGGC
  ACAGTCATCGTCCCTTTTAGGACAGTAGAAGGGACAGCCAAGGGTGGTGGCGAGGACTTTGAAGATGCAT
  ATGGGGAGCTGGAGTTCAAGAATGATGAAACAGTGAAAACCATAAGGGTTAAAATAGTAGATGAGGAGGA
  GTACGAGAGGCAAGAGAATTTCTTCATTGCCCTTGGTGAACCGAAATGGATGGAACGTGGAATATCAGAA
  GTGACAGACAGGAAGCTGACTGTGGAGGAAGAGGAAGCCAAGAGAATAGCAGAGATGGGAAAGCCAGTAT
  TGGGTGAACACCCCAAACTAGAGGTCATCATTGAAGAGTCCTATGAGTTCAAGAGTACAGTGGATAAGCT
  GATCAAGAAGACAAACCTGGCATTGGTTGTGGGGACCCATTCCTGGAGGGACCAGTTCATGGAAGCCATC
  ACTGTTAGTGCAGGAGGGGATGAGGATGAAGACGAATCTGGAGAGGAGAGGCTGCCATCCTGCTTTGACT
  ACGTCATGCACTTCCTGACGGTCTTCTGGAAGGTGCTCTTTGCCTGTGTGCCCCCCACAGAGTACTGCCA
  CGGCTGGGCCTGCTTCGTGGTCTCCATCCTCATTATTGGCATGCTCACCGCCATCATCGGGGACCTGGCC
  TCTCACTTCGGCTGCACCATCGGGCTCAAGGATTCGGTCACAGCTGTTGTTTTTGTGGCATTCGGCACCT
  CTGTGCCAGATACATTTGCCAGCAAAGCCGCTGCCCTGCAGGACGTGTATGCAGATGCTTCCATTGGCAA
  CGTCACAGGCAGTAATGCCGTCAATGTCTTCCTGGGTATTGGTTTGGCCTGGTCCGTGGCCGCCATCTAC
  TGGGCCATGCAGGGACAGGAGTTCCATGTGTCCGCTGGCACTCTGGCCTTCTCGGTCACTCTTTTCACCA
  TCTTTGCATTTGTCTGCCTCAGTGTGCTCTTGTATCGTCGGCGGCCCCATCTGGGCGGGGAGCTCGGAGG
  TCCTCGTGGCTGCAAGCTTGCCACGACATGGCTCTTTGTGAGCCTATGGCTCCTCTACATACTATTTGCC
  ACGCTGGAGGCCTACTGCTACATCAAGGGGTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001167920
• Insert Size: 2766 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167920.1, NP_001161392.1
• RefSeq Size: 4943 bp
• RefSeq ORF: 2766 bp
• Locus ID: 110893
• UniProt ID: S4R2P9
• Cytogenetics: 12 37.44 cM
• Gene Summary: Mediates the electrogenic exchange of Ca(2+) against Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes. Contributes to cellular Ca(2+) homeostasis in excitable cells, both in muscle and in brain (PubMed:14722618, PubMed:21593315). In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum. SLC8A3 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline (PubMed:14722618, PubMed:21593315). Contributes to Ca(2+) transport during excitation-contraction coupling in muscle (PubMed:14722618). In neurons, contributes to the rapid decrease of cytoplasmic Ca(2+) levels back to baseline after neuronal activation, and thereby contributes to modulate synaptic plasticity, learning and memory (PubMed:21593315). Required for normal oligodendrocyte differentiation and for normal myelination (PubMed:21959935). Mediates Ca(2+) efflux from mitochondria and contributes to mitochondrial Ca(2+) ion homeostasis (PubMed:24616101). Isoform 1 displays higher calcium exchanger activity than isoform 2, probably because isoform 1 has a lower threshold for activation by cytoplasmic Ca(2+) (PubMed:24616101).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1.