Pde6c (NM_001170959) Mouse Untagged Clone

CAT#: MC220883

Pde6c (untagged) - Mouse phosphodiesterase 6C, cGMP specific, cone, alpha prime (Pde6c), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pde6c
• Synonyms: cpfl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220883 representing NM_001170959
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGGTGAGATCAGCCAAGAGGCCGTGGAGCGCTACCTGGAGAAGAACCCTTGTTTTGCCAAAGAGTATT
  TTGACAAGAAACTACGGGTGGAGGCACTGGGGGTGATCTTCAAAAACAGCCATGCAGGGGTCCAGACCGG
  CTTGTCGCTCCCTGAGATGACACAGGTGGAGGAGTCGGCCGTGTGCCTGGAGCTACTGCAGTGTATGCAG
  GACGAGGCCGGAAGCGCGGAGCAGATGGCTCACCGGGCACTGCAGAGGCTCGCTCAACTTCTGCAGGCTG
  ATTGCTGCAGTATGTTCTCGTGCAGAGCAAGAAACGGCATACCAGAGGTGGCCTCCAGGCTGCTGAACGT
  CACCCCAACTTCTAAGTTTGAGGACAACCTCGTGGCCCCTGACAGAGAAGTTGTATTTCCGTTGGACATT
  GGGATAGTTGGCTGGGTCGCTCATGTGAAGAAAGCCCTTAATGTCTCAGATGTCAAAAAGAACAGCCATT
  TTTCTGACTTTATGGACAAGCAAACTGGATATGTCACCAGGAATCTGCTAGCAGTCCCGATCGTGGCAGG
  CAAGGAGGTCCTTGCTGTGGTCATGGCAGTGAACAAAATCAGCGCTCCCGAGTTTTCCAAACAGGATGAG
  GAGGTCTTTTCTAAGTACCTCAGCTTTGTTGCTGTCGCACTAAGGCTGCAGCACACCAGTTACCTGTACA
  GTGTGGAGTCCCGGAGAAGCCAGATCCTTATGTGGTCAGCCAATAAAGTATTCGAAGAGCTCACTGATGT
  TGAGCGACAGTTTCACAAGGCACTGTACACCATCAGGACGTACCTGAACTGCGATCGCTACTCCATTGGT
  CTTCTGGACATGACCAAGGAGAAGGAATTTTACGATGAGTGGCCAATCAAGCTTGGAGAAGTTGAGCCTT
  ATAAAGGACCAAAGACTCCAGATGGCAGGGAAATCATCTTCTATAAAATCATCGATTACATTTTACATGG
  AAAAGAAGAGATCAACGTGATTCCGTCACCTCCAGCAGACCACTGGACGCTTGTTAGTGGATTGCCAACA
  TACGTTGCAGAAAATGGATTTATCTGTAACATGCTGAATGCCCCTGCAGATGAGTACTTCACATTTCAGA
  AAGGACCGGTAGATGAGACTGGCTGGGTCATTAAAAATGTCTTGTCCCTGCCTATTGTCAACAAGAAAGA
  AGACATCGTGGGTGTAGCTACGTTTTACAACCGGAAGGATGGGAAACCCTTTGATGAGCATGATGAACAT
  ATCACTGAGACTTTAACCCAGTTTCTTGGATGGTCTCTTTTAAACACCGATACCTATGAGCGAGTGAACA
  AGCTGGAAAGCAGAAAGGACATTGCTCAGGAAATGGTTATGAACCTCACAAAAGCCACCCCGGATGAAAT
  CAGCTCTATTCTGAAGTTTAAAGAGAAGTTAAATGTAGAGGTAATTGAAGAGTGTGAAGAGAGACAGCTC
  CTGGCCATATTGAAGGAGGACTTGCCGGACCCGAGAACAGCAGACCTGTACGAGTTCTGTTTCAGCGACT
  TCCCCATTACGGAGCACGAGTTGGTTAAATGCGGGCTGAGGCTGTTTTTGGAGATAAACGTGGTGGAGAA
  ATTCAAAGTCCCTGTAGAGGTTCTTACAAGGTGGATGTACACAGTCCGGAAAGGGTACCGTCCTGTCACC
  TACCACAACTGGCGGCACGGATTCAACGTGGGGCAGACCATGTTCACTTTGTTAATGACAGGAAGACTAA
  AGAAATACTATACAGACCTTGAAGCCTTTGCCATGCTCGCTGCTGCTTTCTGCCATGACATTGACCACAG
  AGGCACCAATAACTTGTACCAAATGAAGTCCACATCTCCACTTGCAAGGCTTCATGGGACTTCCATTTTA
  GAGCGACATCACCTGGAATATAGCAAGACTCTCTTGCAGGATGAGAGTTTAAACATCTTCCAGAATCTAA
  ACAAGCGGCAGTTTGAAACGGTGATCCATTTGTTCGAGGTTGCAATAATAGCAACTGACCTGGCACTCTA
  TTTCAAGAAGAGAACCATGTTTCAAAAGATCGTTGATACCTGCGAGCAGATGCAAAGTGAAGAAGAAACC
  ATCAAATACGTAACCTCCGACCCGACCAAAAAGGAAGTCATCATGGCCATGATGATGACTGCCTGTGACC
  TGTCTGCCATCACCAAGCCCTGGGAGGTGCAGAGTCAGCCTATGATGGACAGAAGCAAAAAGGATGAACT
  CCCGAAACTTCAAGTGGGATTTATTGATTTTGTTTGTACTTTCGTATATAAGGAGTTCTCTCGGTTTCAT
  GGAGAGATCACACCCATGCTGAACGGCCTTCAGAATAACAGGGTGGAATGGAAATCCCTGGCGGAGGAGT
  ACGAGGCGAAGGTGAAGGTGACTGAAGAAGAGGCGGGAAAGCAGGAAGAAGAAGCCTCAGATGGAAAAGC
  TGCCACAGATTTAGGAGGCAGCGCCGAAGACAAAAAGTCCAAAACATGCTTAATGTTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: AscI-MluI
• ACCN: NM_001170959
• Insert Size: 2511 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170959.1, NP_001164430.1
• RefSeq Size: 2892 bp
• RefSeq ORF: 2511 bp
• Locus ID: 110855
• UniProt ID: Q91ZQ1
• Cytogenetics: 19 C2
• Gene Summary: As cone-specific cGMP phosphodiesterase, it plays an essential role in light detection and cone phototransduction by rapidly decreasing intracellular levels of cGMP.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.