Unc5a (BC058084) Mouse Untagged Clone

CAT#: MC220873

Unc5a (untagged) - Mouse unc-5 homolog A (C. elegans) (cDNA clone MGC:66671 IMAGE:6813463), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Unc5a
• Synonyms: mKIAA1976; Unc5h1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC058084
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTCCGGCCCGGCCTGTGGCCAGCGCTCCTGGGCATAGTCCTCACTGCCTGGCTTCGTGGTTCGG
  GTGCCCAGCAGAGTGCCACAGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTGCCCCACTTCCT
  GGTAGAGCCGGAGGACGTGTACATTGTCAAGAACAAGCCCGTGCTGCTGGTGTGCAAGGCTGTGCCCGCC
  ACCCAGATCTTCTTCAAGTGCAACGGGGAATGGGTTCGCCAGGTCGATCACGTCATTGAACGCAGCACTG
  ACGGCAGCAGCGGATTGCCAACCATGGAGGTCCGGATCAACGTATCAAGGCAGCAGGTCGAGAAAGTGTT
  TGGGCTGGAGGAGTACTGGTGCCAGTGTGTGGCATGGAGCTCCTCAGGAACCACCAAAAGCCAGAAGGCC
  TACATCCGGATTGCCTATTTGCGCAAGAACTTTGAGCAGGAGCCGCTGGCCAAGGAAGTGTCACTGGAGC
  AAGGCATTGTGCTACCTTGTCGCCCCCCGGAAGGAATCCCCCCAGCTGAGGTGGAGTGGCTCCGAAATGA
  GGACCTCGTGGACCCCTCCCTCGACCCCAATGTGTACATCACACGGGAGCACAGCCTAGTCGTGCGGCAG
  GCCCGCCTGGCCGACACTCCCAACTACACCTGCGTGGCCAAGAACATCGTGGCCCGTCGCCGAAGCGCCT
  CTGCGGCCGTCATTGTTTATGTGGATGGGAGCTGGAGCCCATGGAGTAAGTGGTCAGCCTGCGGGCTTGA
  CTGCACCCACTGGCGGAGCCGGGAGTGCTCCGACCCAGCGCCCCGCAACGGAGGTGAGGAGTGCCGGGGT
  GCTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGCCTGCACACCTCTTCCGGCCCCGAGGACGTGG
  CTCTCTACATCGGCCTCGTCGCCGTGGCCGTGTGCCTCATCTTGCTGCTGCTGGTCCTCGTCCTCATCTA
  CTGCCGCAAGAAGGAAGGACTGGACTCAGACGTGGCTGACTCATCCATCCTTACCTCAGGCTTCCAGCCT
  GTCAGCATCAAGCCCAGCAAAGCAGACAATCCCCATCTGCTCACCATCCAACCGGACCTCAGCACCACCA
  CGACCACCTACCAGGGCAGCCTGTGTCCCCGGCAGGATGGACCCAGCCCCAAGTTCCAGCTCTCTAATGG
  TCACCTGCTCAGCCCACTGGGCAGTGGCCGCCATACGCTGCACCACAGCTCCCCCACCTCTGAGGCTGAG
  GACTTCGTCTCCCGCCTCTCCACCCAAAACTACTTTCGTTCTCTGCCCCGCGGTACCAGCAACATGGCCT
  ATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTAACACAGGAATCAGCCTCCTCATACCCCC
  GGACGCCATCCCCCGAGGAAAGATCTACGAGATCTACCTCACTCTGCACAAGCCAGAAGACGTGAGGTTG
  CCCCTAGCTGGCTGTCAGACCCTGCTGAGTCCTATCGTTAGCTGTGGGCCCCCAGGAGTCCTGCTCACCC
  GGCCAGTCATCCTTGCCATGGACCACTGCGGGGAGCCCAGTCCCGACAGCTGGAGCCTGCGCCTCAAAAA
  GCAGTCCTGTGAGGGCAGCTGGGAGGACGTGCTGCACCTTGGTGAGGAGTCGCCCTCTCATCTCTACTAC
  TGCCAGCTGGAGGCCGGGGCCTGCTATGTCTTTACCGAGCAGCTAGGCCGCTTTGCCCTGGTGGGAGAGG
  CCCTCAGCGTGGCTGCCACCAAGCGCCTCAGGCTCCTTCTGTTTGCCCCTGTGGCCTGTACGTCCCTCGA
  GTACAACATCCGAGTGTACTGCCTGCACGACACCCACGATGCTCTCAAGGAGGTGGTGCAGCTGGAGAAG
  CAGCTGGGTGGACAGCTGATCCAGGAGCCCCGTGTCCTGCACTTCAAAGACAGTTACCACAACCTACGTC
  TGTCCATCCACGACGTGCCCAGCTCCCTGTGGAAGAGCAAGCTCCTTGTCAGCTACCAGGAGATCCCTTT
  TTACCACATCTGGAATGGCACTCAGCAGTATCTGCACTGCACCTTCACCCTGGAGCGCGTCAATGCCAGC
  ACCAGCGACCTGGCCTGCAAGGTGTGGGTGTGGCAGGTGGAGGGAGATGGACAGAGCTTCAACATCAACT
  TTAACATCACTAAGGACACGAGGTTTGCTGAAATGCTGGCTCTGGAGAGTGAAGGGGGGGTCCCAGCCCT
  GGTGGGCCCCAGTGCCTTCAAGATCCCCTTCCTCATTCGGCAAAAGATCATTACCAGCCTGGACCCACCC
  TGCAGCCGGGGCGCCGACTGGCGAACTCTAGCCCAGAAACTTCACCTGGACAGCCATCTTAGCTTCTTTG
  CCTCCAAGCCCAGCCCTACAGCCATGATCCTCAACCTATGGGAGGCGCGGCACTTCCCCAACGGCAACCT
  CGGCCAGCTGGCCGCAGCTGTGGCCGGACTGGGCCAGCCAGATGCTGGCCTCTTCACCGTGTCAGAGGCC
  GAGTGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC058084
• Insert Size: 2529 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058084, AAH58084
• RefSeq Size: 3844 bp
• RefSeq ORF: 2528 bp
• Locus ID: 107448
• Cytogenetics: 13 B1
• Gene Summary: Receptor for netrin required for axon guidance. Functions in the netrin signaling pathway and promotes neurite outgrowth in response to NTN1. Mediates axon repulsion of neuronal growth cones in the developing nervous system in response to netrin. Axon repulsion in growth cones may be mediated by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.[UniProtKB/Swiss-Prot Function]